Carbohydrate-binding domain CBM63 of microbial expansin-like BsEXLX1 facilitates the adsorption of expansin-related proteins to hemicelluloses in plant secondary cell walls. Sivan, P., Dahiya, D., Jabalera, Y., Koitto, T., Perez-Jimenez, R., Mellerowicz, E. J., Master, E., & Vilaplana, F. Biotechnology for Biofuels and Bioproducts, 18(1):70, July, 2025. ![Carbohydrate-binding domain CBM63 of microbial expansin-like BsEXLX1 facilitates the adsorption of expansin-related proteins to hemicelluloses in plant secondary cell walls [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Overcoming lignocellulose recalcitrance to enzymatic or chemical processing is a prerequisite for biorefinery applications. Expansins and loosenins are non-lytic proteins that could assist reducing this recalcitrance by disrupting the intermolecular contacts between plant cell wall components. Here, immunolocalization with fluorescence and transmission electron microscopy (TEM) were used to study the ability of a Bacillus subtilis expansin-like protein (BsEXLX1), a Phanerochaete carnosa loosenin protein (PcaLOOL12) and a fusion protein of PcaLOOL12 with the carbohydrate-binding module 63 (CBM63) of BsEXLX1 (i.e., PcaLOOL12-CBM63) to bind secondary cell walls (SCW) of aspen fibres, including fresh aspen wood, milled wood fibres (MWF) and MWF subjected to subcritical water extraction.
@article{sivan_carbohydrate-binding_2025,
title = {Carbohydrate-binding domain {CBM63} of microbial expansin-like {BsEXLX1} facilitates the adsorption of expansin-related proteins to hemicelluloses in plant secondary cell walls},
volume = {18},
issn = {2731-3654},
url = {https://doi.org/10.1186/s13068-025-02674-x},
doi = {10.1186/s13068-025-02674-x},
abstract = {Overcoming lignocellulose recalcitrance to enzymatic or chemical processing is a prerequisite for biorefinery applications. Expansins and loosenins are non-lytic proteins that could assist reducing this recalcitrance by disrupting the intermolecular contacts between plant cell wall components. Here, immunolocalization with fluorescence and transmission electron microscopy (TEM) were used to study the ability of a Bacillus subtilis expansin-like protein (BsEXLX1), a Phanerochaete carnosa loosenin protein (PcaLOOL12) and a fusion protein of PcaLOOL12 with the carbohydrate-binding module 63 (CBM63) of BsEXLX1 (i.e., PcaLOOL12-CBM63) to bind secondary cell walls (SCW) of aspen fibres, including fresh aspen wood, milled wood fibres (MWF) and MWF subjected to subcritical water extraction.},
number = {1},
urldate = {2025-07-25},
journal = {Biotechnology for Biofuels and Bioproducts},
author = {Sivan, Pramod and Dahiya, Deepika and Jabalera, Ylenia and Koitto, Taru and Perez-Jimenez, Raul and Mellerowicz, Ewa J. and Master, Emma and Vilaplana, Francisco},
month = jul,
year = {2025},
keywords = {Expansin, Hemicelluloses, Loosenin, Secondary cell wall},
pages = {70},
}
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