Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification. Sivan, P., Urbancsok, J., Donev, E. N., Derba-Maceluch, M., Barbut, F. R., Yassin, Z., Gandla, M. L., Mitra, M., Heinonen, S. E., Šimura, J., Cermanová, K., Karady, M., Scheepers, G., Jönsson, L. J., Master, E. R., Vilaplana, F., & Mellerowicz, E. J. Plant Biotechnology Journal. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.14487![Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Wood of broad-leaf tree species is a valued source of renewable biomass for biorefinery and a target for genetic improvement efforts to reduce its recalcitrance. Glucuronoxylan (GX) plays a key role in recalcitrance through its interactions with cellulose and lignin. To reduce recalcitrance, we modified wood GX by expressing GH10 and GH11 endoxylanases from Aspergillus nidulans in hybrid aspen (Populus tremula L. × tremuloides Michx.) and targeting the enzymes to cell wall. The xylanases reduced tree height, modified cambial activity by increasing phloem and reducing xylem production, and reduced secondary wall deposition. Xylan molecular weight was decreased, and the spacing between acetyl and MeGlcA side chains was reduced in transgenic lines. The transgenic trees produced hypolignified xylem having thin secondary walls and deformed vessels. Glucose yields of enzymatic saccharification without pretreatment almost doubled indicating decreased recalcitrance. The transcriptomics, hormonomics and metabolomics data provided evidence for activation of cytokinin and ethylene signalling pathways, decrease in ABA levels, transcriptional suppression of lignification and a subset of secondary wall biosynthetic program, including xylan glucuronidation and acetylation machinery. Several candidate genes for perception of impairment in xylan integrity were detected. These candidates could provide a new target for uncoupling negative growth effects from reduced recalcitrance. In conclusion, our study supports the hypothesis that xylan modification generates intrinsic signals and evokes novel pathways regulating tree growth and secondary wall biosynthesis.
@article{sivan_modification_nodate,
title = {Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification},
volume = {n/a},
copyright = {© 2024 The Author(s). Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley \& Sons Ltd.},
issn = {1467-7652},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.14487},
doi = {10.1111/pbi.14487},
abstract = {Wood of broad-leaf tree species is a valued source of renewable biomass for biorefinery and a target for genetic improvement efforts to reduce its recalcitrance. Glucuronoxylan (GX) plays a key role in recalcitrance through its interactions with cellulose and lignin. To reduce recalcitrance, we modified wood GX by expressing GH10 and GH11 endoxylanases from Aspergillus nidulans in hybrid aspen (Populus tremula L. × tremuloides Michx.) and targeting the enzymes to cell wall. The xylanases reduced tree height, modified cambial activity by increasing phloem and reducing xylem production, and reduced secondary wall deposition. Xylan molecular weight was decreased, and the spacing between acetyl and MeGlcA side chains was reduced in transgenic lines. The transgenic trees produced hypolignified xylem having thin secondary walls and deformed vessels. Glucose yields of enzymatic saccharification without pretreatment almost doubled indicating decreased recalcitrance. The transcriptomics, hormonomics and metabolomics data provided evidence for activation of cytokinin and ethylene signalling pathways, decrease in ABA levels, transcriptional suppression of lignification and a subset of secondary wall biosynthetic program, including xylan glucuronidation and acetylation machinery. Several candidate genes for perception of impairment in xylan integrity were detected. These candidates could provide a new target for uncoupling negative growth effects from reduced recalcitrance. In conclusion, our study supports the hypothesis that xylan modification generates intrinsic signals and evokes novel pathways regulating tree growth and secondary wall biosynthesis.},
language = {en},
number = {n/a},
urldate = {2024-10-25},
journal = {Plant Biotechnology Journal},
author = {Sivan, Pramod and Urbancsok, János and Donev, Evgeniy N. and Derba-Maceluch, Marta and Barbut, Félix R. and Yassin, Zakiya and Gandla, Madhavi L. and Mitra, Madhusree and Heinonen, Saara E. and Šimura, Jan and Cermanová, Kateřina and Karady, Michal and Scheepers, Gerhard and Jönsson, Leif J. and Master, Emma R. and Vilaplana, Francisco and Mellerowicz, Ewa J.},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.14487},
keywords = {Glucuronoxylan, fungal xylanases, lignocellulose, secondary cell wall, transgenic aspen, wood development},
}
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Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.","issn":"1467-7652","url":"https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.14487","doi":"10.1111/pbi.14487","abstract":"Wood of broad-leaf tree species is a valued source of renewable biomass for biorefinery and a target for genetic improvement efforts to reduce its recalcitrance. Glucuronoxylan (GX) plays a key role in recalcitrance through its interactions with cellulose and lignin. To reduce recalcitrance, we modified wood GX by expressing GH10 and GH11 endoxylanases from Aspergillus nidulans in hybrid aspen (Populus tremula L. × tremuloides Michx.) and targeting the enzymes to cell wall. The xylanases reduced tree height, modified cambial activity by increasing phloem and reducing xylem production, and reduced secondary wall deposition. 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Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley \\& Sons Ltd.},\n\tissn = {1467-7652},\n\turl = {https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.14487},\n\tdoi = {10.1111/pbi.14487},\n\tabstract = {Wood of broad-leaf tree species is a valued source of renewable biomass for biorefinery and a target for genetic improvement efforts to reduce its recalcitrance. Glucuronoxylan (GX) plays a key role in recalcitrance through its interactions with cellulose and lignin. To reduce recalcitrance, we modified wood GX by expressing GH10 and GH11 endoxylanases from Aspergillus nidulans in hybrid aspen (Populus tremula L. × tremuloides Michx.) and targeting the enzymes to cell wall. The xylanases reduced tree height, modified cambial activity by increasing phloem and reducing xylem production, and reduced secondary wall deposition. Xylan molecular weight was decreased, and the spacing between acetyl and MeGlcA side chains was reduced in transgenic lines. The transgenic trees produced hypolignified xylem having thin secondary walls and deformed vessels. Glucose yields of enzymatic saccharification without pretreatment almost doubled indicating decreased recalcitrance. The transcriptomics, hormonomics and metabolomics data provided evidence for activation of cytokinin and ethylene signalling pathways, decrease in ABA levels, transcriptional suppression of lignification and a subset of secondary wall biosynthetic program, including xylan glucuronidation and acetylation machinery. Several candidate genes for perception of impairment in xylan integrity were detected. These candidates could provide a new target for uncoupling negative growth effects from reduced recalcitrance. 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