Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications. Sivan, P., Urbancsok, J., Gandla, M. L., Donev, E. N., Derba-Maceluch, M., Roach, M., Jönsson, L. J., Vilaplana, F., & Mellerowicz, E. J. Biotechnology for Biofuels and Bioproducts, 19(1):82, October, 2026. ![Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The pattern of xylan backbone substitution by 4-O-methylglucuronic acid (mGlcA) and O-acetyl groups generated during biosynthesis in the Golgi apparatus plays a key role in molecular interactions between secondary cell wall polymers in hardwoods, thus regulating biorefinery properties of woody biomass. This study aims at comparing the effects of two different approaches to reduce xylan glucuronidation, targeting either xylan biosynthesis by RNAi suppression of GLUCURONIC SUBSTITUTION OF XYLAN1 genes (denoted Gux1RNAi) or by xylan post-synthetic modification in the secondary cell wall by apoplastic wood-specific expression of a fungal α-glucuronidase from family GH67 (denoted WP:GH67) in hybrid aspen (Populus tremula L. x tremuloides Michx.). The effects of reducing xylan glucuronidation by these two approaches on xylan acetylation and solubility, and recalcitrance of wood to enzymatic hydrolysis were compared.
@article{sivan_comparison_2026,
title = {Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications},
volume = {19},
issn = {2731-3654},
url = {https://doi.org/10.1186/s13068-026-02830-x},
doi = {10.1186/s13068-026-02830-x},
abstract = {The pattern of xylan backbone substitution by 4-O-methylglucuronic acid (mGlcA) and O-acetyl groups generated during biosynthesis in the Golgi apparatus plays a key role in molecular interactions between secondary cell wall polymers in hardwoods, thus regulating biorefinery properties of woody biomass. This study aims at comparing the effects of two different approaches to reduce xylan glucuronidation, targeting either xylan biosynthesis by RNAi suppression of GLUCURONIC SUBSTITUTION OF XYLAN1 genes (denoted Gux1RNAi) or by xylan post-synthetic modification in the secondary cell wall by apoplastic wood-specific expression of a fungal α-glucuronidase from family GH67 (denoted WP:GH67) in hybrid aspen (Populus tremula L. x tremuloides Michx.). The effects of reducing xylan glucuronidation by these two approaches on xylan acetylation and solubility, and recalcitrance of wood to enzymatic hydrolysis were compared.},
language = {en},
number = {1},
urldate = {2026-10-22},
journal = {Biotechnology for Biofuels and Bioproducts},
author = {Sivan, Pramod and Urbancsok, János and Gandla, Madhavi L. and Donev, Evgeniy N. and Derba-Maceluch, Marta and Roach, Melissa and Jönsson, Leif J. and Vilaplana, Francisco and Mellerowicz, Ewa J.},
month = oct,
year = {2026},
keywords = {GUX1, Glucuronidation, Glucuronoxylan, Populus, Saccharification, Secondary cell wall, Transgenic trees, Wood chemistry, Xylan acetylation, α-glucuronidase},
pages = {82},
}
Downloads: 0
{"_id":"e5CZ4XJ5bpzFL93Ja","bibbaseid":"sivan-urbancsok-gandla-donev-derbamaceluch-roach-jnsson-vilaplana-etal-comparisonofsyntheticandpostsyntheticmoleculargeneticmethodsofreducingxylanglucuronidationinhybridaspenforbiorefineryapplications-2026","author_short":["Sivan, P.","Urbancsok, J.","Gandla, M. L.","Donev, E. N.","Derba-Maceluch, M.","Roach, M.","Jönsson, L. J.","Vilaplana, F.","Mellerowicz, E. J."],"bibdata":{"bibtype":"article","type":"article","title":"Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications","volume":"19","issn":"2731-3654","url":"https://doi.org/10.1186/s13068-026-02830-x","doi":"10.1186/s13068-026-02830-x","abstract":"The pattern of xylan backbone substitution by 4-O-methylglucuronic acid (mGlcA) and O-acetyl groups generated during biosynthesis in the Golgi apparatus plays a key role in molecular interactions between secondary cell wall polymers in hardwoods, thus regulating biorefinery properties of woody biomass. This study aims at comparing the effects of two different approaches to reduce xylan glucuronidation, targeting either xylan biosynthesis by RNAi suppression of GLUCURONIC SUBSTITUTION OF XYLAN1 genes (denoted Gux1RNAi) or by xylan post-synthetic modification in the secondary cell wall by apoplastic wood-specific expression of a fungal α-glucuronidase from family GH67 (denoted WP:GH67) in hybrid aspen (Populus tremula L. x tremuloides Michx.). The effects of reducing xylan glucuronidation by these two approaches on xylan acetylation and solubility, and recalcitrance of wood to enzymatic hydrolysis were compared.","language":"en","number":"1","urldate":"2026-10-22","journal":"Biotechnology for Biofuels and Bioproducts","author":[{"propositions":[],"lastnames":["Sivan"],"firstnames":["Pramod"],"suffixes":[]},{"propositions":[],"lastnames":["Urbancsok"],"firstnames":["János"],"suffixes":[]},{"propositions":[],"lastnames":["Gandla"],"firstnames":["Madhavi","L."],"suffixes":[]},{"propositions":[],"lastnames":["Donev"],"firstnames":["Evgeniy","N."],"suffixes":[]},{"propositions":[],"lastnames":["Derba-Maceluch"],"firstnames":["Marta"],"suffixes":[]},{"propositions":[],"lastnames":["Roach"],"firstnames":["Melissa"],"suffixes":[]},{"propositions":[],"lastnames":["Jönsson"],"firstnames":["Leif","J."],"suffixes":[]},{"propositions":[],"lastnames":["Vilaplana"],"firstnames":["Francisco"],"suffixes":[]},{"propositions":[],"lastnames":["Mellerowicz"],"firstnames":["Ewa","J."],"suffixes":[]}],"month":"October","year":"2026","keywords":"GUX1, Glucuronidation, Glucuronoxylan, Populus, Saccharification, Secondary cell wall, Transgenic trees, Wood chemistry, Xylan acetylation, α-glucuronidase","pages":"82","bibtex":"@article{sivan_comparison_2026,\n\ttitle = {Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications},\n\tvolume = {19},\n\tissn = {2731-3654},\n\turl = {https://doi.org/10.1186/s13068-026-02830-x},\n\tdoi = {10.1186/s13068-026-02830-x},\n\tabstract = {The pattern of xylan backbone substitution by 4-O-methylglucuronic acid (mGlcA) and O-acetyl groups generated during biosynthesis in the Golgi apparatus plays a key role in molecular interactions between secondary cell wall polymers in hardwoods, thus regulating biorefinery properties of woody biomass. This study aims at comparing the effects of two different approaches to reduce xylan glucuronidation, targeting either xylan biosynthesis by RNAi suppression of GLUCURONIC SUBSTITUTION OF XYLAN1 genes (denoted Gux1RNAi) or by xylan post-synthetic modification in the secondary cell wall by apoplastic wood-specific expression of a fungal α-glucuronidase from family GH67 (denoted WP:GH67) in hybrid aspen (Populus tremula L. x tremuloides Michx.). The effects of reducing xylan glucuronidation by these two approaches on xylan acetylation and solubility, and recalcitrance of wood to enzymatic hydrolysis were compared.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2026-10-22},\n\tjournal = {Biotechnology for Biofuels and Bioproducts},\n\tauthor = {Sivan, Pramod and Urbancsok, János and Gandla, Madhavi L. and Donev, Evgeniy N. and Derba-Maceluch, Marta and Roach, Melissa and Jönsson, Leif J. and Vilaplana, Francisco and Mellerowicz, Ewa J.},\n\tmonth = oct,\n\tyear = {2026},\n\tkeywords = {GUX1, Glucuronidation, Glucuronoxylan, Populus, Saccharification, Secondary cell wall, Transgenic trees, Wood chemistry, Xylan acetylation, α-glucuronidase},\n\tpages = {82},\n}\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n","author_short":["Sivan, P.","Urbancsok, J.","Gandla, M. L.","Donev, E. N.","Derba-Maceluch, M.","Roach, M.","Jönsson, L. J.","Vilaplana, F.","Mellerowicz, E. J."],"key":"sivan_comparison_2026","id":"sivan_comparison_2026","bibbaseid":"sivan-urbancsok-gandla-donev-derbamaceluch-roach-jnsson-vilaplana-etal-comparisonofsyntheticandpostsyntheticmoleculargeneticmethodsofreducingxylanglucuronidationinhybridaspenforbiorefineryapplications-2026","role":"author","urls":{"Paper":"https://doi.org/10.1186/s13068-026-02830-x"},"keyword":["GUX1","Glucuronidation","Glucuronoxylan","Populus","Saccharification","Secondary cell wall","Transgenic trees","Wood chemistry","Xylan acetylation","α-glucuronidase"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs"],"keywords":["gux1","glucuronidation","glucuronoxylan","populus","saccharification","secondary cell wall","transgenic trees","wood chemistry","xylan acetylation","α-glucuronidase"],"search_terms":["comparison","synthetic","post","synthetic","molecular","genetic","methods","reducing","xylan","glucuronidation","hybrid","aspen","biorefinery","applications","sivan","urbancsok","gandla","donev","derba-maceluch","roach","jönsson","vilaplana","mellerowicz"],"title":"Comparison of synthetic and post-synthetic molecular genetic methods of reducing xylan glucuronidation in hybrid aspen for biorefinery applications","year":2026}