Molecular treatments to reduce catabolic effects in human meniscus explant models. Sjögren, A., Lindblom, K., Turkiewicz, A., Englund, M., & Önnerfjord, P. Osteoarthritis and Cartilage Open, 7(3):100618, September, 2025. doi abstract bibtex OBJECTIVES: 1. To validate catabolic meniscus explant models induced by cytokines: interleukin-6 + interleukin-6 receptor + tumor necrosis factor alpha (IL6/TNF) and oncostatin M + tumor necrosis factor alpha (OSM/TNF). 2. To evaluate three potential anti-catabolic treatments: i) dexamethasone (DEX), ii) a Link-N peptide (Link-N) and iii) a peptide from chondroadherin (CKF). DESIGN: Healthy lateral menisci from deceased donors (n = 6; age = 25-70 years, 4 males, 2 females), were sliced and randomized for experimental groups (combinations of the catabolic models and anti-catabolic treatments) and a control group. Culture media were analyzed, every third day until day 18, by mass spectrometry-based proteomics. Linear mixed effect models were used to estimate differences in protein abundances between groups. RESULTS: A total of 662 proteins were identified in all menisci. Cytokine-treated meniscus explant models showed increased release of osteoarthritis-related proteins such as matrix metalloproteinases (MMPs). For example, MMP1: IL6/TNF vs. ctrl; log2 fold-change 2.2 95 % confidence interval [1.8, 2.5] and OSM/TNF vs. ctrl; log2 fold-change 2.8 [2.4, 3.1]. There was no treatment effect in explant meniscus with the addition of either Link-N or CKF. Treatment effects were, however, evident with the addition of DEX. For example, MMP1: IL6/TNF + DEX vs. ctrl; log2 fold-change -1.8 [-2.2, -1.4] and OSM/TNF + DEX vs. ctrl; log2 fold-change -0.3 [-0.7, 0.04]. CONCLUSION: We confirmed that both catabolic models induce changes in osteoarthritis-related proteins. DEX treatment is effective in mitigating the catabolic response in meniscus explant models and may be further explored for its effects in the treatment of meniscus degeneration.
@article{sjogren_molecular_2025,
title = {Molecular treatments to reduce catabolic effects in human meniscus explant models},
volume = {7},
issn = {2665-9131},
doi = {10.1016/j.ocarto.2025.100618},
abstract = {OBJECTIVES: 1. To validate catabolic meniscus explant models induced by cytokines: interleukin-6 + interleukin-6 receptor + tumor necrosis factor alpha (IL6/TNF) and oncostatin M + tumor necrosis factor alpha (OSM/TNF). 2. To evaluate three potential anti-catabolic treatments: i) dexamethasone (DEX), ii) a Link-N peptide (Link-N) and iii) a peptide from chondroadherin (CKF).
DESIGN: Healthy lateral menisci from deceased donors (n = 6; age = 25-70 years, 4 males, 2 females), were sliced and randomized for experimental groups (combinations of the catabolic models and anti-catabolic treatments) and a control group. Culture media were analyzed, every third day until day 18, by mass spectrometry-based proteomics. Linear mixed effect models were used to estimate differences in protein abundances between groups.
RESULTS: A total of 662 proteins were identified in all menisci. Cytokine-treated meniscus explant models showed increased release of osteoarthritis-related proteins such as matrix metalloproteinases (MMPs). For example, MMP1: IL6/TNF vs. ctrl; log2 fold-change 2.2 95 \% confidence interval [1.8, 2.5] and OSM/TNF vs. ctrl; log2 fold-change 2.8 [2.4, 3.1]. There was no treatment effect in explant meniscus with the addition of either Link-N or CKF. Treatment effects were, however, evident with the addition of DEX. For example, MMP1: IL6/TNF + DEX vs. ctrl; log2 fold-change -1.8 [-2.2, -1.4] and OSM/TNF + DEX vs. ctrl; log2 fold-change -0.3 [-0.7, 0.04].
CONCLUSION: We confirmed that both catabolic models induce changes in osteoarthritis-related proteins. DEX treatment is effective in mitigating the catabolic response in meniscus explant models and may be further explored for its effects in the treatment of meniscus degeneration.},
language = {eng},
number = {3},
journal = {Osteoarthritis and Cartilage Open},
author = {Sjögren, Amanda and Lindblom, Karin and Turkiewicz, Aleksandra and Englund, Martin and Önnerfjord, Patrik},
month = sep,
year = {2025},
keywords = {Explant model, Human tissue, Meniscus, Osteoarthritis, Proteomics},
pages = {100618},
}
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{"_id":"AhgK8Jhac3PobH97S","bibbaseid":"sjgren-lindblom-turkiewicz-englund-nnerfjord-moleculartreatmentstoreducecataboliceffectsinhumanmeniscusexplantmodels-2025","author_short":["Sjögren, A.","Lindblom, K.","Turkiewicz, A.","Englund, M.","Önnerfjord, P."],"bibdata":{"bibtype":"article","type":"article","title":"Molecular treatments to reduce catabolic effects in human meniscus explant models","volume":"7","issn":"2665-9131","doi":"10.1016/j.ocarto.2025.100618","abstract":"OBJECTIVES: 1. To validate catabolic meniscus explant models induced by cytokines: interleukin-6 + interleukin-6 receptor + tumor necrosis factor alpha (IL6/TNF) and oncostatin M + tumor necrosis factor alpha (OSM/TNF). 2. To evaluate three potential anti-catabolic treatments: i) dexamethasone (DEX), ii) a Link-N peptide (Link-N) and iii) a peptide from chondroadherin (CKF). DESIGN: Healthy lateral menisci from deceased donors (n = 6; age = 25-70 years, 4 males, 2 females), were sliced and randomized for experimental groups (combinations of the catabolic models and anti-catabolic treatments) and a control group. Culture media were analyzed, every third day until day 18, by mass spectrometry-based proteomics. Linear mixed effect models were used to estimate differences in protein abundances between groups. RESULTS: A total of 662 proteins were identified in all menisci. Cytokine-treated meniscus explant models showed increased release of osteoarthritis-related proteins such as matrix metalloproteinases (MMPs). For example, MMP1: IL6/TNF vs. ctrl; log2 fold-change 2.2 95 % confidence interval [1.8, 2.5] and OSM/TNF vs. ctrl; log2 fold-change 2.8 [2.4, 3.1]. There was no treatment effect in explant meniscus with the addition of either Link-N or CKF. Treatment effects were, however, evident with the addition of DEX. For example, MMP1: IL6/TNF + DEX vs. ctrl; log2 fold-change -1.8 [-2.2, -1.4] and OSM/TNF + DEX vs. ctrl; log2 fold-change -0.3 [-0.7, 0.04]. CONCLUSION: We confirmed that both catabolic models induce changes in osteoarthritis-related proteins. DEX treatment is effective in mitigating the catabolic response in meniscus explant models and may be further explored for its effects in the treatment of meniscus degeneration.","language":"eng","number":"3","journal":"Osteoarthritis and Cartilage Open","author":[{"propositions":[],"lastnames":["Sjögren"],"firstnames":["Amanda"],"suffixes":[]},{"propositions":[],"lastnames":["Lindblom"],"firstnames":["Karin"],"suffixes":[]},{"propositions":[],"lastnames":["Turkiewicz"],"firstnames":["Aleksandra"],"suffixes":[]},{"propositions":[],"lastnames":["Englund"],"firstnames":["Martin"],"suffixes":[]},{"propositions":[],"lastnames":["Önnerfjord"],"firstnames":["Patrik"],"suffixes":[]}],"month":"September","year":"2025","keywords":"Explant model, Human tissue, Meniscus, Osteoarthritis, Proteomics","pages":"100618","bibtex":"@article{sjogren_molecular_2025,\n\ttitle = {Molecular treatments to reduce catabolic effects in human meniscus explant models},\n\tvolume = {7},\n\tissn = {2665-9131},\n\tdoi = {10.1016/j.ocarto.2025.100618},\n\tabstract = {OBJECTIVES: 1. To validate catabolic meniscus explant models induced by cytokines: interleukin-6 + interleukin-6 receptor + tumor necrosis factor alpha (IL6/TNF) and oncostatin M + tumor necrosis factor alpha (OSM/TNF). 2. To evaluate three potential anti-catabolic treatments: i) dexamethasone (DEX), ii) a Link-N peptide (Link-N) and iii) a peptide from chondroadherin (CKF).\nDESIGN: Healthy lateral menisci from deceased donors (n = 6; age = 25-70 years, 4 males, 2 females), were sliced and randomized for experimental groups (combinations of the catabolic models and anti-catabolic treatments) and a control group. Culture media were analyzed, every third day until day 18, by mass spectrometry-based proteomics. Linear mixed effect models were used to estimate differences in protein abundances between groups.\nRESULTS: A total of 662 proteins were identified in all menisci. Cytokine-treated meniscus explant models showed increased release of osteoarthritis-related proteins such as matrix metalloproteinases (MMPs). For example, MMP1: IL6/TNF vs. ctrl; log2 fold-change 2.2 95 \\% confidence interval [1.8, 2.5] and OSM/TNF vs. ctrl; log2 fold-change 2.8 [2.4, 3.1]. There was no treatment effect in explant meniscus with the addition of either Link-N or CKF. Treatment effects were, however, evident with the addition of DEX. For example, MMP1: IL6/TNF + DEX vs. ctrl; log2 fold-change -1.8 [-2.2, -1.4] and OSM/TNF + DEX vs. ctrl; log2 fold-change -0.3 [-0.7, 0.04].\nCONCLUSION: We confirmed that both catabolic models induce changes in osteoarthritis-related proteins. DEX treatment is effective in mitigating the catabolic response in meniscus explant models and may be further explored for its effects in the treatment of meniscus degeneration.},\n\tlanguage = {eng},\n\tnumber = {3},\n\tjournal = {Osteoarthritis and Cartilage Open},\n\tauthor = {Sjögren, Amanda and Lindblom, Karin and Turkiewicz, Aleksandra and Englund, Martin and Önnerfjord, Patrik},\n\tmonth = sep,\n\tyear = {2025},\n\tkeywords = {Explant model, Human tissue, Meniscus, Osteoarthritis, Proteomics},\n\tpages = {100618},\n}\n\n","author_short":["Sjögren, A.","Lindblom, K.","Turkiewicz, A.","Englund, M.","Önnerfjord, P."],"key":"sjogren_molecular_2025","id":"sjogren_molecular_2025","bibbaseid":"sjgren-lindblom-turkiewicz-englund-nnerfjord-moleculartreatmentstoreducecataboliceffectsinhumanmeniscusexplantmodels-2025","role":"author","urls":{},"keyword":["Explant model","Human tissue","Meniscus","Osteoarthritis","Proteomics"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://api.zotero.org/groups/5155143/items?key=IVTAjWy1U5EkGJqE2Z2qQCfh&format=bibtex&limit=100","dataSources":["LPTeGao77ndnG4Tks"],"keywords":["explant model","human tissue","meniscus","osteoarthritis","proteomics"],"search_terms":["molecular","treatments","reduce","catabolic","effects","human","meniscus","explant","models","sjögren","lindblom","turkiewicz","englund","önnerfjord"],"title":"Molecular treatments to reduce catabolic effects in human meniscus explant models","year":2025}