Induction of apoptosis in human glioma cell lines of various grades through the ROS-mediated mitochondrial pathway and caspase activation by Rhaponticum carthamoides transformed root extract. Skała, E., Kowalczyk, T., Toma, M., Szemraj, J., Radek, M., Pytel, D., Wieczfinska, J., Wysokińska, H., Śliwiński, T., & Sitarek, P. Molecular and Cellular Biochemistry, December, 2017. doi abstract bibtex The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. These results indicate that the TR extract possesses anticancer activity by inhibiting glioma cell proliferation and inducing apoptotic cell death, and may be used as a promising anticancer agent.
@article{skala_induction_2017,
title = {Induction of apoptosis in human glioma cell lines of various grades through the {ROS}-mediated mitochondrial pathway and caspase activation by {Rhaponticum} carthamoides transformed root extract},
issn = {1573-4919},
doi = {10.1007/s11010-017-3254-z},
abstract = {The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. These results indicate that the TR extract possesses anticancer activity by inhibiting glioma cell proliferation and inducing apoptotic cell death, and may be used as a promising anticancer agent.},
language = {eng},
journal = {Molecular and Cellular Biochemistry},
author = {Skała, Ewa and Kowalczyk, Tomasz and Toma, Monika and Szemraj, Janusz and Radek, Maciej and Pytel, Dariusz and Wieczfinska, Joanna and Wysokińska, Halina and Śliwiński, Tomasz and Sitarek, Przemysław},
month = dec,
year = {2017},
pmid = {29238899},
keywords = {Apoptosis, Bax, Bcl-2, Caffeic acid derivatives, Caspase-3, Caspase-9 gene expressions, Human glioma cells, Rhaponticum carthamoides, Tp53, Transformed roots},
}
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{"_id":"s3DFp3i4DMvd24L2J","bibbaseid":"skaa-kowalczyk-toma-szemraj-radek-pytel-wieczfinska-wysokiska-etal-inductionofapoptosisinhumangliomacelllinesofvariousgradesthroughtherosmediatedmitochondrialpathwayandcaspaseactivationbyrhaponticumcarthamoidestransformedrootextract-2017","author_short":["Skała, E.","Kowalczyk, T.","Toma, M.","Szemraj, J.","Radek, M.","Pytel, D.","Wieczfinska, J.","Wysokińska, H.","Śliwiński, T.","Sitarek, P."],"bibdata":{"bibtype":"article","type":"article","title":"Induction of apoptosis in human glioma cell lines of various grades through the ROS-mediated mitochondrial pathway and caspase activation by Rhaponticum carthamoides transformed root extract","issn":"1573-4919","doi":"10.1007/s11010-017-3254-z","abstract":"The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. These results indicate that the TR extract possesses anticancer activity by inhibiting glioma cell proliferation and inducing apoptotic cell death, and may be used as a promising anticancer agent.","language":"eng","journal":"Molecular and Cellular Biochemistry","author":[{"propositions":[],"lastnames":["Skała"],"firstnames":["Ewa"],"suffixes":[]},{"propositions":[],"lastnames":["Kowalczyk"],"firstnames":["Tomasz"],"suffixes":[]},{"propositions":[],"lastnames":["Toma"],"firstnames":["Monika"],"suffixes":[]},{"propositions":[],"lastnames":["Szemraj"],"firstnames":["Janusz"],"suffixes":[]},{"propositions":[],"lastnames":["Radek"],"firstnames":["Maciej"],"suffixes":[]},{"propositions":[],"lastnames":["Pytel"],"firstnames":["Dariusz"],"suffixes":[]},{"propositions":[],"lastnames":["Wieczfinska"],"firstnames":["Joanna"],"suffixes":[]},{"propositions":[],"lastnames":["Wysokińska"],"firstnames":["Halina"],"suffixes":[]},{"propositions":[],"lastnames":["Śliwiński"],"firstnames":["Tomasz"],"suffixes":[]},{"propositions":[],"lastnames":["Sitarek"],"firstnames":["Przemysław"],"suffixes":[]}],"month":"December","year":"2017","pmid":"29238899","keywords":"Apoptosis, Bax, Bcl-2, Caffeic acid derivatives, Caspase-3, Caspase-9 gene expressions, Human glioma cells, Rhaponticum carthamoides, Tp53, Transformed roots","bibtex":"@article{skala_induction_2017,\n\ttitle = {Induction of apoptosis in human glioma cell lines of various grades through the {ROS}-mediated mitochondrial pathway and caspase activation by {Rhaponticum} carthamoides transformed root extract},\n\tissn = {1573-4919},\n\tdoi = {10.1007/s11010-017-3254-z},\n\tabstract = {The present study is the first investigation of the inhibitory effect of Rhaponticum carthamoides transformed roots (TR) extract on the proliferation of grade II and III human glioma cells. TR extract showed the cytotoxic effect and inhibited the colony formation of both glioma cell lines in dose-dependent manner. The root extract induced apoptosis by increasing of the reactive oxygen species (about threefold compared to the control cells) leading to a disruption of mitochondrial membrane potential. Additionally, the mRNA levels of the apoptotic factors such as Bax, Tp53, caspase-3, and caspase-9 were observed to increase. 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