Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution. Skalický, V., Antoniadi, I., Pěnčík, A., Chamrád, I., Lenobel, R., Kubeš, M. F., Zatloukal, M., Žukauskaitė, A., Strnad, M., Ljung, K., & Novák, O. The Plant Journal, 116(6):1825–1841, September, 2023. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.16456![Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Auxins and cytokinins are two major families of phytohormones that control most aspects of plant growth, development and plasticity. Their distribution in plants has been described, but the importance of cell- and subcellular-type specific phytohormone homeostasis remains undefined. Herein, we revealed auxin and cytokinin distribution maps showing their different organelle-specific allocations within the Arabidopsis plant cell. To do so, we have developed Fluorescence-Activated multi-Organelle Sorting (FAmOS), an innovative subcellular fractionation technique based on flow cytometric principles. FAmOS allows the simultaneous sorting of four differently labelled organelles based on their individual light scatter and fluorescence parameters while ensuring hormone metabolic stability. Our data showed different subcellular distribution of auxin and cytokinins, revealing the formation of phytohormone gradients that have been suggested by the subcellular localization of auxin and cytokinin transporters, receptors and metabolic enzymes. Both hormones showed enrichment in vacuoles, while cytokinins were also accumulated in the endoplasmic reticulum.
@article{skalicky_fluorescence-activated_2023,
title = {Fluorescence-activated multi-organelle mapping of subcellular plant hormone distribution},
volume = {116},
copyright = {© 2023 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley \& Sons Ltd.},
issn = {1365-313X},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.16456},
doi = {10.1111/tpj.16456},
abstract = {Auxins and cytokinins are two major families of phytohormones that control most aspects of plant growth, development and plasticity. Their distribution in plants has been described, but the importance of cell- and subcellular-type specific phytohormone homeostasis remains undefined. Herein, we revealed auxin and cytokinin distribution maps showing their different organelle-specific allocations within the Arabidopsis plant cell. To do so, we have developed Fluorescence-Activated multi-Organelle Sorting (FAmOS), an innovative subcellular fractionation technique based on flow cytometric principles. FAmOS allows the simultaneous sorting of four differently labelled organelles based on their individual light scatter and fluorescence parameters while ensuring hormone metabolic stability. Our data showed different subcellular distribution of auxin and cytokinins, revealing the formation of phytohormone gradients that have been suggested by the subcellular localization of auxin and cytokinin transporters, receptors and metabolic enzymes. Both hormones showed enrichment in vacuoles, while cytokinins were also accumulated in the endoplasmic reticulum.},
language = {en},
number = {6},
urldate = {2023-12-22},
journal = {The Plant Journal},
author = {Skalický, Vladimír and Antoniadi, Ioanna and Pěnčík, Aleš and Chamrád, Ivo and Lenobel, René and Kubeš, Martin F. and Zatloukal, Marek and Žukauskaitė, Asta and Strnad, Miroslav and Ljung, Karin and Novák, Ondřej},
month = sep,
year = {2023},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/tpj.16456},
keywords = {Arabidopsis thaliana, Auxin, LC–MS/MS, cytokinin, flow cytometry, subcellular fractionation, subcellular homeostasis, technical advances},
pages = {1825--1841},
}
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