Age, disease severity and ethnicity influence humoral responses in a multi-ethnic COVID-19 cohort. Smith, M., Abdesselem, H. B, Mullins, M., Tan, T., Nel, A. J M, Al-Nesf, M. A Y, Bensmail, I., Majbour, N. K, Vaikath, N. N, Naik, A., Ouararhni, K., Mohamed-Ali, V., Al-Maadheed, M., Schell, D. T, Baros-Steyl, S. S, Anuar, N. D, Ismail, N. H, Morris, P. E, Mamat, R. N R, Rosli, N. S M, Anwar, A., Ellan, K., Zain, R. M, Burgers, W. A, Mayne, E. S, El-Agnaf, O. M A, & Blackburn, J. M Viruses, 13(5):786, Viruses, apr, 2021. ![Age, disease severity and ethnicity influence humoral responses in a multi-ethnic COVID-19 cohort [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions. Quantitative data from the microarray provided complete differentiation between cases and pre-pandemic controls (100% sensitivity and specificity) in a case-control cohort (n = 100). We then assessed the influence of disease severity, age, and ethnicity on the strength and breadth of the humoral response in a multi-ethnic cohort (n = 138). As expected, patients with severe disease showed significantly higher antibody titers and interestingly also had significantly broader epitope coverage. A significant increase in antibody titer and epitope coverage was observed with increasing age, in both mild and severe disease, which is promising for vaccine efficacy in older individuals. Additionally, we observed significant differences in the breadth and strength of the humoral immune response in relation to ethnicity, which may reflect differences in genetic and lifestyle factors. Furthermore, our data enabled localization of the immuno-dominant epitope to the C-terminal structural domain of the viral nucleocapsid protein in two independent cohorts. Overall, we have designed, validated, and tested an advanced serological assay that enables accurate quantitation of the humoral response post natural infection and that has revealed unexpected differences in the magnitude and epitope utilization within a population.
@article{Smith2021,
abstract = {The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions. Quantitative data from the microarray provided complete differentiation between cases and pre-pandemic controls (100{\%} sensitivity and specificity) in a case-control cohort (n = 100). We then assessed the influence of disease severity, age, and ethnicity on the strength and breadth of the humoral response in a multi-ethnic cohort (n = 138). As expected, patients with severe disease showed significantly higher antibody titers and interestingly also had significantly broader epitope coverage. A significant increase in antibody titer and epitope coverage was observed with increasing age, in both mild and severe disease, which is promising for vaccine efficacy in older individuals. Additionally, we observed significant differences in the breadth and strength of the humoral immune response in relation to ethnicity, which may reflect differences in genetic and lifestyle factors. Furthermore, our data enabled localization of the immuno-dominant epitope to the C-terminal structural domain of the viral nucleocapsid protein in two independent cohorts. Overall, we have designed, validated, and tested an advanced serological assay that enables accurate quantitation of the humoral response post natural infection and that has revealed unexpected differences in the magnitude and epitope utilization within a population.},
author = {Smith, Muneerah and Abdesselem, Houari B and Mullins, Michelle and Tan, Ti-Myen and Nel, Andrew J M and Al-Nesf, Maryam A Y and Bensmail, Ilham and Majbour, Nour K and Vaikath, Nishant N and Naik, Adviti and Ouararhni, Khalid and Mohamed-Ali, Vidya and Al-Maadheed, Mohammed and Schell, Darien T and Baros-Steyl, Seanantha S and Anuar, Nur D and Ismail, Nur H and Morris, Priscilla E and Mamat, Raja N R and Rosli, Nurul S M and Anwar, Arif and Ellan, Kavithambigai and Zain, Rozainanee M and Burgers, Wendy A and Mayne, Elizabeth S and El-Agnaf, Omar M A and Blackburn, Jonathan M},
doi = {10.3390/v13050786},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Smith et al. - 2021 - Age, disease severity and ethnicity influence humoral responses in a multi-ethnic COVID-19 cohort.pdf:pdf},
issn = {1999-4915},
journal = {Viruses},
keywords = {Houari B Abdesselem,Jonathan M Blackburn,MEDLINE,Muneerah Smith,NCBI,NIH,NLM,National Center for Biotechnology Information,National Institutes of Health,National Library of Medicine,OA,PubMed Abstract,doi:10.3390/v13050786,fund{\_}not{\_}ack,original,pmid:33925055},
mendeley-tags = {OA,fund{\_}not{\_}ack,original},
month = {apr},
number = {5},
pages = {786},
pmid = {33925055},
publisher = {Viruses},
title = {{Age, disease severity and ethnicity influence humoral responses in a multi-ethnic COVID-19 cohort}},
url = {http://www.ncbi.nlm.nih.gov/pubmed/33925055},
volume = {13},
year = {2021}
}
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