Structural Fingerprinting of Protein Aggregates by Dynamic Nuclear Polarization-Enhanced Solid-State NMR at Natural Isotopic Abundance. Smith, A., Märker, K., Piretra, T., Boatz, J., Matlahov, I., Kodali, R., Hediger, S., Van Der Wel, P., & De Paëpe, G. Journal of the American Chemical Society, 140(44):14576–14580, 2018. doi abstract bibtex 1 download A pathological hallmark of Huntington's disease (HD) is the formation of neuronal protein deposits containing mutant huntingtin fragments with expanded polyglutamine (polyQ) domains. Prior studies have shown the strengths of solid-state NMR (ssNMR) to probe the atomic structure of such aggregates, but have required in vitro isotopic labeling. Herein, we present an approach for the structural fingerprinting of fibrils through ssNMR at natural isotopic abundance (NA). These methods will enable the spectroscopic fingerprinting of unlabeled (e.g., ex vivo) protein aggregates and the extraction of valuable new long-range 13C-13C distance constraints.
@article{smith_structural_2018,
title = {Structural {Fingerprinting} of {Protein} {Aggregates} by {Dynamic} {Nuclear} {Polarization}-{Enhanced} {Solid}-{State} {NMR} at {Natural} {Isotopic} {Abundance}},
volume = {140},
doi = {10.1021/jacs.8b09002},
abstract = {A pathological hallmark of Huntington's disease (HD) is the formation of neuronal protein deposits containing mutant huntingtin fragments with expanded polyglutamine (polyQ) domains. Prior studies have shown the strengths of solid-state NMR (ssNMR) to probe the atomic structure of such aggregates, but have required in vitro isotopic labeling. Herein, we present an approach for the structural fingerprinting of fibrils through ssNMR at natural isotopic abundance (NA). These methods will enable the spectroscopic fingerprinting of unlabeled (e.g., ex vivo) protein aggregates and the extraction of valuable new long-range 13C-13C distance constraints.},
number = {44},
journal = {Journal of the American Chemical Society},
author = {Smith, A.N. and Märker, K. and Piretra, T. and Boatz, J.C. and Matlahov, I. and Kodali, R. and Hediger, S. and Van Der Wel, P.C.A. and De Paëpe, G.},
year = {2018},
pages = {14576--14580},
}
Downloads: 1
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