The nitrate-sensing NasST system regulates nitrous oxide reductase and periplasmic nitrate reductase in Bradyrhizobium japonicum. Sánchez, C., Itakura, M., Okubo, T., Matsumoto, T., Yoshikawa, H., Gotoh, A., Hidaka, M., Uchida, T., & Minamisawa, K. Environmental Microbiology, 2014.
Paper abstract bibtex © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. Summary: The soybean endosymbiont Bradyrhizobium japonicum is able to scavenge the greenhouse gas N 2 O through the N 2 O reductase (Nos). In previous research, N 2 O emission from soybean rhizosphere was mitigated by B.japonicumNos ++ strains (mutants with increased Nos activity). Here, we report the mechanism underlying the Nos ++ phenotype. Comparative analysis of Nos ++ mutant genomes showed that mutation of bll4572 resulted in Nos ++ phenotype. bll4572 encodes NasS, the nitrate (NO 3 - )-sensor of the two-component NasST regulatory system. Transcriptional analyses of nosZ (encoding Nos) and other genes from the denitrification process in nasS and nasST mutants showed that, in the absence of NO 3 - , nasS mutation induces nosZ and nap (periplasmic nitrate reductase) via nasT. NO 3 - addition dissociated the NasS-NasT complex in vitro, suggesting the release of the activator NasT. Disruption of nasT led to a marked decrease in nosZ and nap transcription in cells incubated in the presence of NO 3 - . Thus, although NasST is known to regulate the NO 3 - -mediated response of NO 3 - assimilation genes in bacteria, our results show that NasST regulates the NO 3 - -mediated response of nosZ and napE genes, from the dissimilatory denitrification pathway, in B.japonicum.
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title = {The nitrate-sensing NasST system regulates nitrous oxide reductase and periplasmic nitrate reductase in Bradyrhizobium japonicum},
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year = {2014},
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abstract = {© 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. Summary: The soybean endosymbiont Bradyrhizobium japonicum is able to scavenge the greenhouse gas N 2 O through the N 2 O reductase (Nos). In previous research, N 2 O emission from soybean rhizosphere was mitigated by B.japonicumNos ++ strains (mutants with increased Nos activity). Here, we report the mechanism underlying the Nos ++ phenotype. Comparative analysis of Nos ++ mutant genomes showed that mutation of bll4572 resulted in Nos ++ phenotype. bll4572 encodes NasS, the nitrate (NO 3 - )-sensor of the two-component NasST regulatory system. Transcriptional analyses of nosZ (encoding Nos) and other genes from the denitrification process in nasS and nasST mutants showed that, in the absence of NO 3 - , nasS mutation induces nosZ and nap (periplasmic nitrate reductase) via nasT. NO 3 - addition dissociated the NasS-NasT complex in vitro, suggesting the release of the activator NasT. Disruption of nasT led to a marked decrease in nosZ and nap transcription in cells incubated in the presence of NO 3 - . Thus, although NasST is known to regulate the NO 3 - -mediated response of NO 3 - assimilation genes in bacteria, our results show that NasST regulates the NO 3 - -mediated response of nosZ and napE genes, from the dissimilatory denitrification pathway, in B.japonicum.},
bibtype = {article},
author = {Sánchez, C. and Itakura, M. and Okubo, T. and Matsumoto, T. and Yoshikawa, H. and Gotoh, A. and Hidaka, M. and Uchida, T. and Minamisawa, K.},
journal = {Environmental Microbiology},
number = {10}
}
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Summary: The soybean endosymbiont Bradyrhizobium japonicum is able to scavenge the greenhouse gas N 2 O through the N 2 O reductase (Nos). In previous research, N 2 O emission from soybean rhizosphere was mitigated by B.japonicumNos ++ strains (mutants with increased Nos activity). Here, we report the mechanism underlying the Nos ++ phenotype. Comparative analysis of Nos ++ mutant genomes showed that mutation of bll4572 resulted in Nos ++ phenotype. bll4572 encodes NasS, the nitrate (NO 3 - )-sensor of the two-component NasST regulatory system. Transcriptional analyses of nosZ (encoding Nos) and other genes from the denitrification process in nasS and nasST mutants showed that, in the absence of NO 3 - , nasS mutation induces nosZ and nap (periplasmic nitrate reductase) via nasT. NO 3 - addition dissociated the NasS-NasT complex in vitro, suggesting the release of the activator NasT. Disruption of nasT led to a marked decrease in nosZ and nap transcription in cells incubated in the presence of NO 3 - . Thus, although NasST is known to regulate the NO 3 - -mediated response of NO 3 - assimilation genes in bacteria, our results show that NasST regulates the NO 3 - -mediated response of nosZ and napE genes, from the dissimilatory denitrification pathway, in B.japonicum.","bibtype":"article","author":"Sánchez, C. and Itakura, M. and Okubo, T. and Matsumoto, T. and Yoshikawa, H. and Gotoh, A. and Hidaka, M. and Uchida, T. and Minamisawa, K.","journal":"Environmental Microbiology","number":"10","bibtex":"@article{\n title = {The nitrate-sensing NasST system regulates nitrous oxide reductase and periplasmic nitrate reductase in Bradyrhizobium japonicum},\n type = {article},\n year = {2014},\n identifiers = {[object Object]},\n volume = {16},\n id = {9873c9da-bd16-3dfc-a7e0-974b92b2763e},\n created = {2018-02-28T22:42:48.585Z},\n file_attached = {true},\n profile_id = {a39b8b7f-45f4-3d11-94d2-dbc38be2487d},\n last_modified = {2019-10-11T06:26:59.135Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n folder_uuids = {fb148bbf-95dd-484e-a015-b6ec3f305c75},\n private_publication = {true},\n abstract = {© 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. 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