@article{
 title = {The nitrate-sensing NasST system regulates nitrous oxide reductase and periplasmic nitrate reductase in Bradyrhizobium japonicum},
 type = {article},
 year = {2014},
 identifiers = {[object Object]},
 volume = {16},
 id = {9873c9da-bd16-3dfc-a7e0-974b92b2763e},
 created = {2018-02-28T22:42:48.585Z},
 file_attached = {true},
 profile_id = {a39b8b7f-45f4-3d11-94d2-dbc38be2487d},
 last_modified = {2019-10-11T06:26:59.135Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {false},
 hidden = {false},
 folder_uuids = {fb148bbf-95dd-484e-a015-b6ec3f305c75},
 private_publication = {true},
 abstract = {© 2014 Society for Applied Microbiology and John Wiley  &  Sons Ltd. Summary: The soybean endosymbiont Bradyrhizobium japonicum is able to scavenge the greenhouse gas N 2 O through the N 2 O reductase (Nos). In previous research, N 2 O emission from soybean rhizosphere was mitigated by B.japonicumNos ++ strains (mutants with increased Nos activity). Here, we report the mechanism underlying the Nos ++ phenotype. Comparative analysis of Nos ++ mutant genomes showed that mutation of bll4572 resulted in Nos ++ phenotype. bll4572 encodes NasS, the nitrate (NO 3 - )-sensor of the two-component NasST regulatory system. Transcriptional analyses of nosZ (encoding Nos) and other genes from the denitrification process in nasS and nasST mutants showed that, in the absence of NO 3 - , nasS mutation induces nosZ and nap (periplasmic nitrate reductase) via nasT. NO 3 - addition dissociated the NasS-NasT complex in vitro, suggesting the release of the activator NasT. Disruption of nasT led to a marked decrease in nosZ and nap transcription in cells incubated in the presence of NO 3 - . Thus, although NasST is known to regulate the NO 3 - -mediated response of NO 3 - assimilation genes in bacteria, our results show that NasST regulates the NO 3 - -mediated response of nosZ and napE genes, from the dissimilatory denitrification pathway, in B.japonicum.},
 bibtype = {article},
 author = {Sánchez, C. and Itakura, M. and Okubo, T. and Matsumoto, T. and Yoshikawa, H. and Gotoh, A. and Hidaka, M. and Uchida, T. and Minamisawa, K.},
 journal = {Environmental Microbiology},
 number = {10}
}

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Summary: The soybean endosymbiont Bradyrhizobium japonicum is able to scavenge the greenhouse gas N 2 O through the N 2 O reductase (Nos). In previous research, N 2 O emission from soybean rhizosphere was mitigated by B.japonicumNos ++ strains (mutants with increased Nos activity). Here, we report the mechanism underlying the Nos ++ phenotype. Comparative analysis of Nos ++ mutant genomes showed that mutation of bll4572 resulted in Nos ++ phenotype. bll4572 encodes NasS, the nitrate (NO 3 - )-sensor of the two-component NasST regulatory system. Transcriptional analyses of nosZ (encoding Nos) and other genes from the denitrification process in nasS and nasST mutants showed that, in the absence of NO 3 - , nasS mutation induces nosZ and nap (periplasmic nitrate reductase) via nasT. NO 3 - addition dissociated the NasS-NasT complex in vitro, suggesting the release of the activator NasT. Disruption of nasT led to a marked decrease in nosZ and nap transcription in cells incubated in the presence of NO 3 - . Thus, although NasST is known to regulate the NO 3 - -mediated response of NO 3 - assimilation genes in bacteria, our results show that NasST regulates the NO 3 - -mediated response of nosZ and napE genes, from the dissimilatory denitrification pathway, in B.japonicum.","bibtype":"article","author":"Sánchez, C. and Itakura, M. and Okubo, T. and Matsumoto, T. and Yoshikawa, H. and Gotoh, A. and Hidaka, M. and Uchida, T. and Minamisawa, K.","journal":"Environmental Microbiology","number":"10","bibtex":"@article{\n title = {The nitrate-sensing NasST system regulates nitrous oxide reductase and periplasmic nitrate reductase in Bradyrhizobium japonicum},\n type = {article},\n year = {2014},\n identifiers = {[object Object]},\n volume = {16},\n id = {9873c9da-bd16-3dfc-a7e0-974b92b2763e},\n created = {2018-02-28T22:42:48.585Z},\n file_attached = {true},\n profile_id = {a39b8b7f-45f4-3d11-94d2-dbc38be2487d},\n last_modified = {2019-10-11T06:26:59.135Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n folder_uuids = {fb148bbf-95dd-484e-a015-b6ec3f305c75},\n private_publication = {true},\n abstract = {© 2014 Society for Applied Microbiology and John Wiley & Sons Ltd. Summary: The soybean endosymbiont Bradyrhizobium japonicum is able to scavenge the greenhouse gas N 2 O through the N 2 O reductase (Nos). In previous research, N 2 O emission from soybean rhizosphere was mitigated by B.japonicumNos ++ strains (mutants with increased Nos activity). Here, we report the mechanism underlying the Nos ++ phenotype. Comparative analysis of Nos ++ mutant genomes showed that mutation of bll4572 resulted in Nos ++ phenotype. bll4572 encodes NasS, the nitrate (NO 3 - )-sensor of the two-component NasST regulatory system. Transcriptional analyses of nosZ (encoding Nos) and other genes from the denitrification process in nasS and nasST mutants showed that, in the absence of NO 3 - , nasS mutation induces nosZ and nap (periplasmic nitrate reductase) via nasT. NO 3 - addition dissociated the NasS-NasT complex in vitro, suggesting the release of the activator NasT. Disruption of nasT led to a marked decrease in nosZ and nap transcription in cells incubated in the presence of NO 3 - . Thus, although NasST is known to regulate the NO 3 - -mediated response of NO 3 - assimilation genes in bacteria, our results show that NasST regulates the NO 3 - -mediated response of nosZ and napE genes, from the dissimilatory denitrification pathway, in B.japonicum.},\n bibtype = {article},\n author = {Sánchez, C. and Itakura, M. and Okubo, T. and Matsumoto, T. and Yoshikawa, H. and Gotoh, A. and Hidaka, M. and Uchida, T. and Minamisawa, K.},\n journal = {Environmental Microbiology},\n number = {10}\n}","author_short":["Sánchez, C.","Itakura, M.","Okubo, T.","Matsumoto, T.","Yoshikawa, H.","Gotoh, A.","Hidaka, M.","Uchida, T.","Minamisawa, K."],"urls":{"Paper":"https://bibbase.org/service/mendeley/a39b8b7f-45f4-3d11-94d2-dbc38be2487d/file/4123d05e-bd75-308d-0f7c-3aeea14f81ad/Sánchez_et_al___2014___The_nitrate_sensing_NasST_system_regulates_nitrous_oxide_reductase_and_periplasmic_nitrate_reduct.pdf.pdf"},"bibbaseid":"snchez-itakura-okubo-matsumoto-yoshikawa-gotoh-hidaka-uchida-etal-thenitratesensingnasstsystemregulatesnitrousoxidereductaseandperiplasmicnitratereductaseinbradyrhizobiumjaponicum-2014","role":"author","downloads":0,"html":""},"bibtype":"article","creationDate":"2019-10-11T06:47:49.308Z","downloads":0,"keywords":[],"search_terms":["nitrate","sensing","nasst","system","regulates","nitrous","oxide","reductase","periplasmic","nitrate","reductase","bradyrhizobium","japonicum","sánchez","itakura","okubo","matsumoto","yoshikawa","gotoh","hidaka","uchida","minamisawa"],"title":"The nitrate-sensing NasST system regulates nitrous oxide reductase and periplasmic nitrate reductase in Bradyrhizobium japonicum","year":2014}