Multiplexed microfluidic fluorescence immunoassay with photodiode array signal acquisition for sub-minute and point-of-need detection of mycotoxins

Multiplexed microfluidic fluorescence immunoassay with photodiode array signal acquisition for sub-minute and point-of-need detection of mycotoxins. Soares, R. R. G., Santos, D. R., Pinto, I. F., Azevedo, A. M., Raquel Aires-Barros, M., Chu, V., & Conde, J. P. LAB ON A CHIP, 18(11):1569-1580, JUN 7, 2018.
doi abstract bibtex

Portable, rapid, cost effective and simple analytical tools are in increasing demand to facilitate the routine monitoring of target chemical/biological compounds at the point-of-need. Such devices are highly relevant within the context of food safety, particularly concerning the screening of highly toxic and strictly regulated mycotoxins. To achieve ultrarapid detection of mycotoxins, namely aflatoxin B1, ochratoxin A and deoxynivalenol, at the point-of-need, a novel multiplexed bead-based microfluidic competitive immunosensor, coupled with an array of a-Si:H thin-film photodiodes for integrated fluorescence signal acquisition, is reported. Simultaneously measuring the initial binding rate for each analyte of the sample under analysis against an internal reference, this device provided limits of detection below 1 ng mL(-1) for all mycotoxins in a single-step assay and within 1 minute after mixing the sample under analysis with a fluorescent conjugate. The compatibility of the device with the analysis of mycotoxins spiked in corn samples was further demonstrated after performing a sample preparation procedure based on aqueous two-phase extraction. The short times of analysis and sensitivities in the low ng mL(-1) range make these devices potentially competitive with the lateral flow devices that are currently the standard for this application. Furthermore, this device architecture and concept is amenable of being expanded to other analytes in food safety, biomedical and other applications.

@article{ ISI:000437658200007,
Author = {Soares, Ruben R. G. and Santos, Denis R. and Pinto, Ines F. and Azevedo,
   Ana M. and Raquel Aires-Barros, M. and Chu, Virginia and Conde, Joao P.},
Title = {{Multiplexed microfluidic fluorescence immunoassay with photodiode array
   signal acquisition for sub-minute and point-of-need detection of
   mycotoxins}},
Journal = {{LAB ON A CHIP}},
Year = {{2018}},
Volume = {{18}},
Number = {{11}},
Pages = {{1569-1580}},
Month = {{JUN 7}},
Abstract = {{Portable, rapid, cost effective and simple analytical tools are in
   increasing demand to facilitate the routine monitoring of target
   chemical/biological compounds at the point-of-need. Such devices are
   highly relevant within the context of food safety, particularly
   concerning the screening of highly toxic and strictly regulated
   mycotoxins. To achieve ultrarapid detection of mycotoxins, namely
   aflatoxin B1, ochratoxin A and deoxynivalenol, at the point-of-need, a
   novel multiplexed bead-based microfluidic competitive immunosensor,
   coupled with an array of a-Si:H thin-film photodiodes for integrated
   fluorescence signal acquisition, is reported. Simultaneously measuring
   the initial binding rate for each analyte of the sample under analysis
   against an internal reference, this device provided limits of detection
   below 1 ng mL(-1) for all mycotoxins in a single-step assay and within 1
   minute after mixing the sample under analysis with a fluorescent
   conjugate. The compatibility of the device with the analysis of
   mycotoxins spiked in corn samples was further demonstrated after
   performing a sample preparation procedure based on aqueous two-phase
   extraction. The short times of analysis and sensitivities in the low ng
   mL(-1) range make these devices potentially competitive with the lateral
   flow devices that are currently the standard for this application.
   Furthermore, this device architecture and concept is amenable of being
   expanded to other analytes in food safety, biomedical and other
   applications.}},
DOI = {{10.1039/c8lc00259b}},
ISSN = {{1473-0197}},
EISSN = {{1473-0189}},
ResearcherID-Numbers = {{Soares, Ruben Rafael Goncalves/H-9580-2019
   Conde, Joao Pedro/F-8533-2012
   Azevedo, Ana Margarida/A-2985-2011
   Santos, Denis R./F-4015-2018
   Chu, Virginia/I-6048-2014
   }},
ORCID-Numbers = {{Soares, Ruben Rafael Goncalves/0000-0001-5958-5232
   Conde, Joao Pedro/0000-0002-5677-3024
   Azevedo, Ana Margarida/0000-0001-5659-466X
   Santos, Denis R./0000-0001-7992-9602
   Chu, Virginia/0000-0002-5306-4409
   Pinto, Ines/0000-0002-9714-4742}},
Unique-ID = {{ISI:000437658200007}},
}

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