Resolving isomeric peptide mixtures: a combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library. Srebalus, B.; Hilderbrand, A., E.; Valentine, S., J.; and Clemmer, D., E. Analytical chemistry, 74(1):26-36, 1, 2002.
Resolving isomeric peptide mixtures: a combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library. [pdf]Paper  Resolving isomeric peptide mixtures: a combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library. [link]Website  abstract   bibtex   
A reversed-phase high-performance liquid chromatography (HPLC) separation approach has been combined with ion mobility/time-of-flight (TOF) mass spectrometry in order to characterize a combinatorial peptide library designed to contain 4000 peptides of the general form NH2-Xxx-Xxx-XXX-CO2H, NH2-Ala-Xxx-Xxx-Xxx-CO2H, NH2-Ser-Ala-Xxx-Xxx-Xxx-CO2H and NH2-Leu-Ser-Ala-Xxx-Xxx-Xxx-CO2H (where Xxx represents a randomization over 10 different amino acids: Ala, Arg, Asp, Glu, Gly, Leu, Lys, Phe, Ser, and Val). Addition of the gas-phase mobility separation between the HPLC separation and TOF measurement dimensions makes it possible to resolve many peptide isomers that have identical retention times (and masses).
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 title = {Resolving isomeric peptide mixtures: a combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library.},
 type = {article},
 year = {2002},
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 keywords = {Chromatography, High Pressure Liquid,Combinatorial Chemistry Techniques,Peptide Library,Peptides,Peptides: chemistry,Peptides: isolation & purification,Spectrometry, Mass, Matrix-Assisted Laser Desorpti,Stereoisomerism},
 pages = {26-36},
 volume = {74},
 websites = {http://www.ncbi.nlm.nih.gov/pubmed/11795805},
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 abstract = {A reversed-phase high-performance liquid chromatography (HPLC) separation approach has been combined with ion mobility/time-of-flight (TOF) mass spectrometry in order to characterize a combinatorial peptide library designed to contain 4000 peptides of the general form NH2-Xxx-Xxx-XXX-CO2H, NH2-Ala-Xxx-Xxx-Xxx-CO2H, NH2-Ser-Ala-Xxx-Xxx-Xxx-CO2H and NH2-Leu-Ser-Ala-Xxx-Xxx-Xxx-CO2H (where Xxx represents a randomization over 10 different amino acids: Ala, Arg, Asp, Glu, Gly, Leu, Lys, Phe, Ser, and Val). Addition of the gas-phase mobility separation between the HPLC separation and TOF measurement dimensions makes it possible to resolve many peptide isomers that have identical retention times (and masses).},
 bibtype = {article},
 author = {Srebalus, BarnesCatherineA and Hilderbrand, Amy E and Valentine, Stephen J and Clemmer, David E},
 journal = {Analytical chemistry},
 number = {1}
}
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