An alternative cytokinin biosynthesis pathway. Åstot, C., Dolezal, K., Nordström, A., Wang, Q., Kunkel, T., Moritz, T., Chua, N., & Sandberg, G. Proceedings of the National Academy of Sciences, 97(26):14778–14783, December, 2000. Publisher: National Academy of Sciences Section: Biological Sciences
Paper doi abstract bibtex Studies of de novo cytokinin biosynthesis in isopentenyltransferase (ipt)-transformed Arabidopsis thaliana, involving in vivo deuterium labeling and mass spectrometry, showed that the biosynthetic rate of zeatinriboside-5′-monophosphate was around 66-fold higher than that of isopentenyladenosine-5′-monophosphate (iPMP), the proposed primary product of the Agrobacterium ipt. Double tracer analysis, using [2H6] isopentenyladenosine and deuterium oxide, provided evidence for an alternative, iPMP-independent, biosynthetic pathway for zeatin-type cytokinins, present in both ipt-expressing and wild-type Arabidopsis thaliana. Reduction of the biosynthetic flux in the alternative pathway by use of mevastatin, an inhibitor for 3-hydroxy-3-methylglutaryl CoA reductase, indicated a terpenoid origin for the side-chain precursor of the iPMP independent pathway.
@article{astot_alternative_2000,
title = {An alternative cytokinin biosynthesis pathway},
volume = {97},
copyright = {Copyright © 2000, The National Academy of Sciences},
issn = {0027-8424, 1091-6490},
url = {https://www.pnas.org/content/97/26/14778},
doi = {10/dt77jp},
abstract = {Studies of de novo cytokinin biosynthesis in isopentenyltransferase (ipt)-transformed Arabidopsis thaliana, involving in vivo deuterium labeling and mass spectrometry, showed that the biosynthetic rate of zeatinriboside-5′-monophosphate was around 66-fold higher than that of isopentenyladenosine-5′-monophosphate (iPMP), the proposed primary product of the Agrobacterium ipt. Double tracer analysis, using [2H6] isopentenyladenosine and deuterium oxide, provided evidence for an alternative, iPMP-independent, biosynthetic pathway for zeatin-type cytokinins, present in both ipt-expressing and wild-type Arabidopsis thaliana. Reduction of the biosynthetic flux in the alternative pathway by use of mevastatin, an inhibitor for 3-hydroxy-3-methylglutaryl CoA reductase, indicated a terpenoid origin for the side-chain precursor of the iPMP independent pathway.},
language = {en},
number = {26},
urldate = {2021-11-08},
journal = {Proceedings of the National Academy of Sciences},
author = {Åstot, Crister and Dolezal, Karel and Nordström, Anders and Wang, Qun and Kunkel, Tim and Moritz, Thomas and Chua, Nam-Hai and Sandberg, Göran},
month = dec,
year = {2000},
pmid = {11114204},
note = {Publisher: National Academy of Sciences
Section: Biological Sciences},
pages = {14778--14783},
}