Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells. Subbaramaiah, K, Chung, W J, Michaluart, P, Telang, N, Tanabe, T, Inoue, H, Jang, M, Pezzuto, J M, & Dannenberg, A J The Journal of Biological Chemistry, 273(34):21875–21882, August, 1998. ![Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper abstract bibtex We determined whether resveratrol, a phenolic antioxidant found in grapes and other food products, inhibited phorbol ester (PMA)-mediated induction of COX-2 in human mammary and oral epithelial cells. Treatment of cells with PMA induces COX-2 and causes a marked increase in the production of prostaglandin E2. These effects were inhibited by resveratrol. Resveratrol suppressed PMA-mediated increases in COX-2 mRNA and protein. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with PMA, an effect that was inhibited by resveratrol. PMA caused about a 6-fold increase in COX-2 promoter activity, which was suppressed by resveratrol. Transient transfections utilizing COX-2 promoter deletion constructs and COX-2 promoter constructs, in which specific enhancer elements were mutagenized, indicated that the effects of PMA and resveratrol were mediated via a cyclic AMP response element. Resveratrol inhibited PMA-mediated activation of protein kinase C. Overexpressing protein kinase C-alpha, ERK1, and c-Jun led to 4.7-, 5.1-, and 4-fold increases in COX-2 promoter activity, respectively. These effects also were inhibited by resveratrol. Resveratrol blocked PMA-dependent activation of AP-1-mediated gene expression. In addition to the above effects on gene expression, we found that resveratrol also directly inhibited the activity of COX-2. These data are likely to be important for understanding the anti-cancer and anti-inflammatory properties of resveratrol.
@article{subbaramaiah_resveratrol_1998,
title = {Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells},
volume = {273},
issn = {0021-9258},
url = {http://www.ncbi.nlm.nih.gov/pubmed/9705326},
abstract = {We determined whether resveratrol, a phenolic antioxidant found in grapes and other food products, inhibited phorbol ester (PMA)-mediated induction of COX-2 in human mammary and oral epithelial cells. Treatment of cells with PMA induces COX-2 and causes a marked increase in the production of prostaglandin E2. These effects were inhibited by resveratrol. Resveratrol suppressed PMA-mediated increases in COX-2 mRNA and protein. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with PMA, an effect that was inhibited by resveratrol. PMA caused about a 6-fold increase in COX-2 promoter activity, which was suppressed by resveratrol. Transient transfections utilizing COX-2 promoter deletion constructs and COX-2 promoter constructs, in which specific enhancer elements were mutagenized, indicated that the effects of PMA and resveratrol were mediated via a cyclic AMP response element. Resveratrol inhibited PMA-mediated activation of protein kinase C. Overexpressing protein kinase C-alpha, ERK1, and c-Jun led to 4.7-, 5.1-, and 4-fold increases in COX-2 promoter activity, respectively. These effects also were inhibited by resveratrol. Resveratrol blocked PMA-dependent activation of AP-1-mediated gene expression. In addition to the above effects on gene expression, we found that resveratrol also directly inhibited the activity of COX-2. These data are likely to be important for understanding the anti-cancer and anti-inflammatory properties of resveratrol.},
number = {34},
urldate = {2012-01-04},
journal = {The Journal of Biological Chemistry},
author = {Subbaramaiah, K and Chung, W J and Michaluart, P and Telang, N and Tanabe, T and Inoue, H and Jang, M and Pezzuto, J M and Dannenberg, A J},
month = aug,
year = {1998},
pmid = {9705326},
keywords = {Anti-Inflammatory Agents, Non-Steroidal, Breast, Cells, Cultured, Cyclooxygenase 2, Enzyme Induction, Enzyme Inhibitors, Epithelial Cells, Female, Humans, Isoenzymes, Membrane Proteins, Promoter Regions, Genetic, Prostaglandin-Endoperoxide Synthases, Stilbenes, Tetradecanoylphorbol Acetate, Transcription, Genetic},
pages = {21875--21882},
}
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Resveratrol suppressed PMA-mediated increases in COX-2 mRNA and protein. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with PMA, an effect that was inhibited by resveratrol. PMA caused about a 6-fold increase in COX-2 promoter activity, which was suppressed by resveratrol. Transient transfections utilizing COX-2 promoter deletion constructs and COX-2 promoter constructs, in which specific enhancer elements were mutagenized, indicated that the effects of PMA and resveratrol were mediated via a cyclic AMP response element. Resveratrol inhibited PMA-mediated activation of protein kinase C. Overexpressing protein kinase C-alpha, ERK1, and c-Jun led to 4.7-, 5.1-, and 4-fold increases in COX-2 promoter activity, respectively. These effects also were inhibited by resveratrol. Resveratrol blocked PMA-dependent activation of AP-1-mediated gene expression. In addition to the above effects on gene expression, we found that resveratrol also directly inhibited the activity of COX-2. These data are likely to be important for understanding the anti-cancer and anti-inflammatory properties of resveratrol.","number":"34","urldate":"2012-01-04","journal":"The Journal of Biological Chemistry","author":[{"propositions":[],"lastnames":["Subbaramaiah"],"firstnames":["K"],"suffixes":[]},{"propositions":[],"lastnames":["Chung"],"firstnames":["W","J"],"suffixes":[]},{"propositions":[],"lastnames":["Michaluart"],"firstnames":["P"],"suffixes":[]},{"propositions":[],"lastnames":["Telang"],"firstnames":["N"],"suffixes":[]},{"propositions":[],"lastnames":["Tanabe"],"firstnames":["T"],"suffixes":[]},{"propositions":[],"lastnames":["Inoue"],"firstnames":["H"],"suffixes":[]},{"propositions":[],"lastnames":["Jang"],"firstnames":["M"],"suffixes":[]},{"propositions":[],"lastnames":["Pezzuto"],"firstnames":["J","M"],"suffixes":[]},{"propositions":[],"lastnames":["Dannenberg"],"firstnames":["A","J"],"suffixes":[]}],"month":"August","year":"1998","pmid":"9705326","keywords":"Anti-Inflammatory Agents, Non-Steroidal, Breast, Cells, Cultured, Cyclooxygenase 2, Enzyme Induction, Enzyme Inhibitors, Epithelial Cells, Female, Humans, Isoenzymes, Membrane Proteins, Promoter Regions, Genetic, Prostaglandin-Endoperoxide Synthases, Stilbenes, Tetradecanoylphorbol Acetate, Transcription, Genetic","pages":"21875–21882","bibtex":"@article{subbaramaiah_resveratrol_1998,\n\ttitle = {Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells},\n\tvolume = {273},\n\tissn = {0021-9258},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/9705326},\n\tabstract = {We determined whether resveratrol, a phenolic antioxidant found in grapes and other food products, inhibited phorbol ester (PMA)-mediated induction of COX-2 in human mammary and oral epithelial cells. Treatment of cells with PMA induces COX-2 and causes a marked increase in the production of prostaglandin E2. These effects were inhibited by resveratrol. Resveratrol suppressed PMA-mediated increases in COX-2 mRNA and protein. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with PMA, an effect that was inhibited by resveratrol. PMA caused about a 6-fold increase in COX-2 promoter activity, which was suppressed by resveratrol. Transient transfections utilizing COX-2 promoter deletion constructs and COX-2 promoter constructs, in which specific enhancer elements were mutagenized, indicated that the effects of PMA and resveratrol were mediated via a cyclic AMP response element. Resveratrol inhibited PMA-mediated activation of protein kinase C. Overexpressing protein kinase C-alpha, ERK1, and c-Jun led to 4.7-, 5.1-, and 4-fold increases in COX-2 promoter activity, respectively. These effects also were inhibited by resveratrol. Resveratrol blocked PMA-dependent activation of AP-1-mediated gene expression. In addition to the above effects on gene expression, we found that resveratrol also directly inhibited the activity of COX-2. These data are likely to be important for understanding the anti-cancer and anti-inflammatory properties of resveratrol.},\n\tnumber = {34},\n\turldate = {2012-01-04},\n\tjournal = {The Journal of Biological Chemistry},\n\tauthor = {Subbaramaiah, K and Chung, W J and Michaluart, P and Telang, N and Tanabe, T and Inoue, H and Jang, M and Pezzuto, J M and Dannenberg, A J},\n\tmonth = aug,\n\tyear = {1998},\n\tpmid = {9705326},\n\tkeywords = {Anti-Inflammatory Agents, Non-Steroidal, Breast, Cells, Cultured, Cyclooxygenase 2, Enzyme Induction, Enzyme Inhibitors, Epithelial Cells, Female, Humans, Isoenzymes, Membrane Proteins, Promoter Regions, Genetic, Prostaglandin-Endoperoxide Synthases, Stilbenes, Tetradecanoylphorbol Acetate, Transcription, Genetic},\n\tpages = {21875--21882},\n}\n\n","author_short":["Subbaramaiah, K","Chung, W J","Michaluart, P","Telang, N","Tanabe, T","Inoue, H","Jang, M","Pezzuto, J M","Dannenberg, A J"],"key":"subbaramaiah_resveratrol_1998","id":"subbaramaiah_resveratrol_1998","bibbaseid":"subbaramaiah-chung-michaluart-telang-tanabe-inoue-jang-pezzuto-etal-resveratrolinhibitscyclooxygenase2transcriptionandactivityinphorbolestertreatedhumanmammaryepithelialcells-1998","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/9705326"},"keyword":["Anti-Inflammatory Agents","Non-Steroidal","Breast","Cells","Cultured","Cyclooxygenase 2","Enzyme Induction","Enzyme Inhibitors","Epithelial Cells","Female","Humans","Isoenzymes","Membrane Proteins","Promoter Regions","Genetic","Prostaglandin-Endoperoxide Synthases","Stilbenes","Tetradecanoylphorbol Acetate","Transcription","Genetic"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://api.zotero.org/users/585595/collections/EZA9MKN6/items?key=KhA4GoMeOvxPn5tlpemkMrP3&format=bibtex&limit=100","dataSources":["mLRCv4BM3hCZt8ZRw"],"keywords":["anti-inflammatory agents","non-steroidal","breast","cells","cultured","cyclooxygenase 2","enzyme induction","enzyme inhibitors","epithelial cells","female","humans","isoenzymes","membrane proteins","promoter regions","genetic","prostaglandin-endoperoxide synthases","stilbenes","tetradecanoylphorbol acetate","transcription","genetic"],"search_terms":["resveratrol","inhibits","cyclooxygenase","transcription","activity","phorbol","ester","treated","human","mammary","epithelial","cells","subbaramaiah","chung","michaluart","telang","tanabe","inoue","jang","pezzuto","dannenberg"],"title":"Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells","year":1998}