@ARTICLE{Subramanian2017-ff,
  title    = "Dynamic behaviour of human neuroepithelial cells in the
              developing forebrain",
  author   = "Subramanian, Lakshmi and Bershteyn, Marina and Paredes, Mercedes
              F and Kriegstein, Arnold R",
  abstract = "To understand how diverse progenitor cells contribute to human
              neocortex development, we examined forebrain progenitor behaviour
              using timelapse imaging. Here we find that cell cycle dynamics of
              human neuroepithelial (NE) cells differ from radial glial (RG)
              cells in both primary tissue and in stem cell-derived organoids.
              NE cells undergoing proliferative, symmetric divisions retract
              their basal processes, and both daughter cells regrow a new
              process following cytokinesis. The mitotic retraction of the
              basal process is recapitulated by NE cells in cerebral organoids
              generated from human-induced pluripotent stem cells. In contrast,
              RG cells undergoing vertical cleavage retain their basal fibres
              throughout mitosis, both in primary tissue and in older
              organoids. Our findings highlight developmentally regulated
              changes in mitotic behaviour that may relate to the role of RG
              cells to provide a stable scaffold for neuronal migration, and
              suggest that the transition in mitotic dynamics can be studied in
              organoid models.",
  journal  = "Nat Commun",
  volume   =  8,
  pages    = "14167",
  month    =  jan,
  year     =  2017,
  language = "en"
}

{"_id":"zjp8iBaRDmjmkJs8G","bibbaseid":"subramanian-bershteyn-paredes-kriegstein-dynamicbehaviourofhumanneuroepithelialcellsinthedevelopingforebrain-2017","author_short":["Subramanian, L.","Bershteyn, M.","Paredes, M. F","Kriegstein, A. R"],"bibdata":{"bibtype":"article","type":"article","title":"Dynamic behaviour of human neuroepithelial cells in the developing forebrain","author":[{"propositions":[],"lastnames":["Subramanian"],"firstnames":["Lakshmi"],"suffixes":[]},{"propositions":[],"lastnames":["Bershteyn"],"firstnames":["Marina"],"suffixes":[]},{"propositions":[],"lastnames":["Paredes"],"firstnames":["Mercedes","F"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["Arnold","R"],"suffixes":[]}],"abstract":"To understand how diverse progenitor cells contribute to human neocortex development, we examined forebrain progenitor behaviour using timelapse imaging. Here we find that cell cycle dynamics of human neuroepithelial (NE) cells differ from radial glial (RG) cells in both primary tissue and in stem cell-derived organoids. NE cells undergoing proliferative, symmetric divisions retract their basal processes, and both daughter cells regrow a new process following cytokinesis. The mitotic retraction of the basal process is recapitulated by NE cells in cerebral organoids generated from human-induced pluripotent stem cells. In contrast, RG cells undergoing vertical cleavage retain their basal fibres throughout mitosis, both in primary tissue and in older organoids. Our findings highlight developmentally regulated changes in mitotic behaviour that may relate to the role of RG cells to provide a stable scaffold for neuronal migration, and suggest that the transition in mitotic dynamics can be studied in organoid models.","journal":"Nat Commun","volume":"8","pages":"14167","month":"January","year":"2017","language":"en","bibtex":"@ARTICLE{Subramanian2017-ff,\n title = \"Dynamic behaviour of human neuroepithelial cells in the\n developing forebrain\",\n author = \"Subramanian, Lakshmi and Bershteyn, Marina and Paredes, Mercedes\n F and Kriegstein, Arnold R\",\n abstract = \"To understand how diverse progenitor cells contribute to human\n neocortex development, we examined forebrain progenitor behaviour\n using timelapse imaging. Here we find that cell cycle dynamics of\n human neuroepithelial (NE) cells differ from radial glial (RG)\n cells in both primary tissue and in stem cell-derived organoids.\n NE cells undergoing proliferative, symmetric divisions retract\n their basal processes, and both daughter cells regrow a new\n process following cytokinesis. The mitotic retraction of the\n basal process is recapitulated by NE cells in cerebral organoids\n generated from human-induced pluripotent stem cells. In contrast,\n RG cells undergoing vertical cleavage retain their basal fibres\n throughout mitosis, both in primary tissue and in older\n organoids. Our findings highlight developmentally regulated\n changes in mitotic behaviour that may relate to the role of RG\n cells to provide a stable scaffold for neuronal migration, and\n suggest that the transition in mitotic dynamics can be studied in\n organoid models.\",\n journal = \"Nat Commun\",\n volume = 8,\n pages = \"14167\",\n month = jan,\n year = 2017,\n language = \"en\"\n}\n\n","author_short":["Subramanian, L.","Bershteyn, M.","Paredes, M. F","Kriegstein, A. R"],"key":"Subramanian2017-ff","id":"Subramanian2017-ff","bibbaseid":"subramanian-bershteyn-paredes-kriegstein-dynamicbehaviourofhumanneuroepithelialcellsinthedevelopingforebrain-2017","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":[],"search_terms":["dynamic","behaviour","human","neuroepithelial","cells","developing","forebrain","subramanian","bershteyn","paredes","kriegstein"],"title":"Dynamic behaviour of human neuroepithelial cells in the developing forebrain","year":2017}