FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis. Sun, B., Bhati, K. K., Song, P., Edwards, A., Petri, L., Kruusvee, V., Blaakmeer, A., Dolde, U., Rodrigues, V., Straub, D., Yang, J., Jia, G., & Wenkel, S. PLOS Genetics, 18(9):e1010386, September, 2022. ![FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3’-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3’-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation.
@article{sun_fiona1-mediated_2022,
title = {{FIONA1}-mediated methylation of the 3’{UTR} of {FLC} affects {FLC} transcript levels and flowering in {Arabidopsis}},
volume = {18},
issn = {1553-7404},
url = {https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010386},
doi = {10.1371/journal.pgen.1010386},
abstract = {Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3’-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3’-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation.},
language = {en},
number = {9},
urldate = {2022-11-30},
journal = {PLOS Genetics},
author = {Sun, Bin and Bhati, Kaushal Kumar and Song, Peizhe and Edwards, Ashleigh and Petri, Louise and Kruusvee, Valdeko and Blaakmeer, Anko and Dolde, Ulla and Rodrigues, Vandasue and Straub, Daniel and Yang, Junbo and Jia, Guifang and Wenkel, Stephan},
month = sep,
year = {2022},
keywords = {Arabidopsis thaliana, Flowering plants, Gene expression, Leaves, Messenger RNA, Methylation, Phenotypes, RNA sequencing},
pages = {e1010386},
}
Downloads: 0
{"_id":"3jm4GqW9k3YRdMnEW","bibbaseid":"sun-bhati-song-edwards-petri-kruusvee-blaakmeer-dolde-etal-fiona1mediatedmethylationofthe3utrofflcaffectsflctranscriptlevelsandfloweringinarabidopsis-2022","author_short":["Sun, B.","Bhati, K. K.","Song, P.","Edwards, A.","Petri, L.","Kruusvee, V.","Blaakmeer, A.","Dolde, U.","Rodrigues, V.","Straub, D.","Yang, J.","Jia, G.","Wenkel, S."],"bibdata":{"bibtype":"article","type":"article","title":"FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis","volume":"18","issn":"1553-7404","url":"https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010386","doi":"10.1371/journal.pgen.1010386","abstract":"Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3’-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3’-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation.","language":"en","number":"9","urldate":"2022-11-30","journal":"PLOS Genetics","author":[{"propositions":[],"lastnames":["Sun"],"firstnames":["Bin"],"suffixes":[]},{"propositions":[],"lastnames":["Bhati"],"firstnames":["Kaushal","Kumar"],"suffixes":[]},{"propositions":[],"lastnames":["Song"],"firstnames":["Peizhe"],"suffixes":[]},{"propositions":[],"lastnames":["Edwards"],"firstnames":["Ashleigh"],"suffixes":[]},{"propositions":[],"lastnames":["Petri"],"firstnames":["Louise"],"suffixes":[]},{"propositions":[],"lastnames":["Kruusvee"],"firstnames":["Valdeko"],"suffixes":[]},{"propositions":[],"lastnames":["Blaakmeer"],"firstnames":["Anko"],"suffixes":[]},{"propositions":[],"lastnames":["Dolde"],"firstnames":["Ulla"],"suffixes":[]},{"propositions":[],"lastnames":["Rodrigues"],"firstnames":["Vandasue"],"suffixes":[]},{"propositions":[],"lastnames":["Straub"],"firstnames":["Daniel"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["Junbo"],"suffixes":[]},{"propositions":[],"lastnames":["Jia"],"firstnames":["Guifang"],"suffixes":[]},{"propositions":[],"lastnames":["Wenkel"],"firstnames":["Stephan"],"suffixes":[]}],"month":"September","year":"2022","keywords":"Arabidopsis thaliana, Flowering plants, Gene expression, Leaves, Messenger RNA, Methylation, Phenotypes, RNA sequencing","pages":"e1010386","bibtex":"@article{sun_fiona1-mediated_2022,\n\ttitle = {{FIONA1}-mediated methylation of the 3’{UTR} of {FLC} affects {FLC} transcript levels and flowering in {Arabidopsis}},\n\tvolume = {18},\n\tissn = {1553-7404},\n\turl = {https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010386},\n\tdoi = {10.1371/journal.pgen.1010386},\n\tabstract = {Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3’-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3’-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation.},\n\tlanguage = {en},\n\tnumber = {9},\n\turldate = {2022-11-30},\n\tjournal = {PLOS Genetics},\n\tauthor = {Sun, Bin and Bhati, Kaushal Kumar and Song, Peizhe and Edwards, Ashleigh and Petri, Louise and Kruusvee, Valdeko and Blaakmeer, Anko and Dolde, Ulla and Rodrigues, Vandasue and Straub, Daniel and Yang, Junbo and Jia, Guifang and Wenkel, Stephan},\n\tmonth = sep,\n\tyear = {2022},\n\tkeywords = {Arabidopsis thaliana, Flowering plants, Gene expression, Leaves, Messenger RNA, Methylation, Phenotypes, RNA sequencing},\n\tpages = {e1010386},\n}\n\n","author_short":["Sun, B.","Bhati, K. K.","Song, P.","Edwards, A.","Petri, L.","Kruusvee, V.","Blaakmeer, A.","Dolde, U.","Rodrigues, V.","Straub, D.","Yang, J.","Jia, G.","Wenkel, S."],"key":"sun_fiona1-mediated_2022","id":"sun_fiona1-mediated_2022","bibbaseid":"sun-bhati-song-edwards-petri-kruusvee-blaakmeer-dolde-etal-fiona1mediatedmethylationofthe3utrofflcaffectsflctranscriptlevelsandfloweringinarabidopsis-2022","role":"author","urls":{"Paper":"https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010386"},"keyword":["Arabidopsis thaliana","Flowering plants","Gene expression","Leaves","Messenger RNA","Methylation","Phenotypes","RNA sequencing"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs"],"keywords":["arabidopsis thaliana","flowering plants","gene expression","leaves","messenger rna","methylation","phenotypes","rna sequencing"],"search_terms":["fiona1","mediated","methylation","utr","flc","affects","flc","transcript","levels","flowering","arabidopsis","sun","bhati","song","edwards","petri","kruusvee","blaakmeer","dolde","rodrigues","straub","yang","jia","wenkel"],"title":"FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis","year":2022}