@article{pub.1197684159,
 abstract = {The coupled process of ferric ammonium oxidation (Feammox) and nitrate-dependent ferrous oxidation (NDFO) is a cost-effective nitrogen removal strategy, yet insufficient molecular evidence supports its microbial mechanisms. This study successfully established anaerobic Fe-N coupling systems using anammox sludge and activated sludge as inoculum. Batch experiments and microbial community analysis revealed that two systems achieved similar nitrogen removal, dominated by anammox and Feammox performed by anammox bacteria (AnAOB), with a minor NDFO contribution, and convergently enriched core functional guilds under identical environmental pressures. Genome-resolved metagenomics further indicated that the porin-cytochrome protein complex associated with extracellular electron transfer co-occurred with anammox genes in Brocadia sapporoensis, suggesting its potential Feammox capability. Meanwhile, the iron oxidation gene Cyc2 co-occurred with mtr pathway homologs and complete denitrification genes in IGN3 sp900696555, suggesting its role in NDFO. This genomic evidence supports their dual metabolic capabilities, providing new insights into nitrogen removal in the coupled Feammox-NDFO process.},
 author = {Sun, Hui and Han, Ying and Ren, Shiquan and Zhang, Xinrui and Li, Xinru and Bi, Peng and Chen, Long and Zhu, Liang and Yang, Guangchao and Ma, Jingwei and He, Qiulai},
 date = {2026-01-30},
 doi = {10.1016/j.biortech.2026.134125},
 journal = {Bioresource Technology},
 keywords = {},
 number = {},
 pages = {134125},
 title = {Convergent enrichment of core functional guilds involved in Fe-N metabolism in anammox and activated sludge: Insights into genome-resolved metagenomics},
 url = {https://app.dimensions.ai/details/publication/pub.1197684159},
 volume = {445},
 year = {2026}
}

{"_id":"4eN6somDGFCur6TsW","bibbaseid":"sun-han-ren-zhang-li-bi-chen-zhu-etal-convergentenrichmentofcorefunctionalguildsinvolvedinfenmetabolisminanammoxandactivatedsludgeinsightsintogenomeresolvedmetagenomics-2026","author_short":["Sun, H.","Han, Y.","Ren, S.","Zhang, X.","Li, X.","Bi, P.","Chen, L.","Zhu, L.","Yang, G.","Ma, J.","He, Q."],"bibdata":{"bibtype":"article","type":"article","abstract":"The coupled process of ferric ammonium oxidation (Feammox) and nitrate-dependent ferrous oxidation (NDFO) is a cost-effective nitrogen removal strategy, yet insufficient molecular evidence supports its microbial mechanisms. This study successfully established anaerobic Fe-N coupling systems using anammox sludge and activated sludge as inoculum. Batch experiments and microbial community analysis revealed that two systems achieved similar nitrogen removal, dominated by anammox and Feammox performed by anammox bacteria (AnAOB), with a minor NDFO contribution, and convergently enriched core functional guilds under identical environmental pressures. Genome-resolved metagenomics further indicated that the porin-cytochrome protein complex associated with extracellular electron transfer co-occurred with anammox genes in Brocadia sapporoensis, suggesting its potential Feammox capability. Meanwhile, the iron oxidation gene Cyc2 co-occurred with mtr pathway homologs and complete denitrification genes in IGN3 sp900696555, suggesting its role in NDFO. This genomic evidence supports their dual metabolic capabilities, providing new insights into nitrogen removal in the coupled Feammox-NDFO process.","author":[{"propositions":[],"lastnames":["Sun"],"firstnames":["Hui"],"suffixes":[]},{"propositions":[],"lastnames":["Han"],"firstnames":["Ying"],"suffixes":[]},{"propositions":[],"lastnames":["Ren"],"firstnames":["Shiquan"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Xinrui"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Xinru"],"suffixes":[]},{"propositions":[],"lastnames":["Bi"],"firstnames":["Peng"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Long"],"suffixes":[]},{"propositions":[],"lastnames":["Zhu"],"firstnames":["Liang"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["Guangchao"],"suffixes":[]},{"propositions":[],"lastnames":["Ma"],"firstnames":["Jingwei"],"suffixes":[]},{"propositions":[],"lastnames":["He"],"firstnames":["Qiulai"],"suffixes":[]}],"date":"2026-01-30","doi":"10.1016/j.biortech.2026.134125","journal":"Bioresource Technology","keywords":"","number":"","pages":"134125","title":"Convergent enrichment of core functional guilds involved in Fe-N metabolism in anammox and activated sludge: Insights into genome-resolved metagenomics","url":"https://app.dimensions.ai/details/publication/pub.1197684159","volume":"445","year":"2026","bibtex":"@article{pub.1197684159,\n abstract = {The coupled process of ferric ammonium oxidation (Feammox) and nitrate-dependent ferrous oxidation (NDFO) is a cost-effective nitrogen removal strategy, yet insufficient molecular evidence supports its microbial mechanisms. This study successfully established anaerobic Fe-N coupling systems using anammox sludge and activated sludge as inoculum. Batch experiments and microbial community analysis revealed that two systems achieved similar nitrogen removal, dominated by anammox and Feammox performed by anammox bacteria (AnAOB), with a minor NDFO contribution, and convergently enriched core functional guilds under identical environmental pressures. Genome-resolved metagenomics further indicated that the porin-cytochrome protein complex associated with extracellular electron transfer co-occurred with anammox genes in Brocadia sapporoensis, suggesting its potential Feammox capability. Meanwhile, the iron oxidation gene Cyc2 co-occurred with mtr pathway homologs and complete denitrification genes in IGN3 sp900696555, suggesting its role in NDFO. This genomic evidence supports their dual metabolic capabilities, providing new insights into nitrogen removal in the coupled Feammox-NDFO process.},\n author = {Sun, Hui and Han, Ying and Ren, Shiquan and Zhang, Xinrui and Li, Xinru and Bi, Peng and Chen, Long and Zhu, Liang and Yang, Guangchao and Ma, Jingwei and He, Qiulai},\n date = {2026-01-30},\n doi = {10.1016/j.biortech.2026.134125},\n journal = {Bioresource Technology},\n keywords = {},\n number = {},\n pages = {134125},\n title = {Convergent enrichment of core functional guilds involved in Fe-N metabolism in anammox and activated sludge: Insights into genome-resolved metagenomics},\n url = {https://app.dimensions.ai/details/publication/pub.1197684159},\n volume = {445},\n year = {2026}\n}\n\n","author_short":["Sun, H.","Han, Y.","Ren, S.","Zhang, X.","Li, X.","Bi, P.","Chen, L.","Zhu, L.","Yang, G.","Ma, J.","He, Q."],"key":"pub.1197684159","id":"pub.1197684159","bibbaseid":"sun-han-ren-zhang-li-bi-chen-zhu-etal-convergentenrichmentofcorefunctionalguildsinvolvedinfenmetabolisminanammoxandactivatedsludgeinsightsintogenomeresolvedmetagenomics-2026","role":"author","urls":{"Paper":"https://app.dimensions.ai/details/publication/pub.1197684159"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/4KAZHSd6FGCYJfjqh","dataSources":["qcQTmRGauYEBvS59n"],"keywords":[],"search_terms":["convergent","enrichment","core","functional","guilds","involved","metabolism","anammox","activated","sludge","insights","genome","resolved","metagenomics","sun","han","ren","zhang","li","bi","chen","zhu","yang","ma","he"],"title":"Convergent enrichment of core functional guilds involved in Fe-N metabolism in anammox and activated sludge: Insights into genome-resolved metagenomics","year":2026}