Metagenomic species profiling using universal phylogenetic marker genes. Sunagawa, S., Mende, D., R., Zeller, G., Izquierdo-Carrasco, F., Berger, S., A., Kultima, J., R., Coelho, L., P., Arumugam, M., Tap, J., Nielsen, H., B., Rasmussen, S., Brunak, S., Pedersen, O., Guarner, F., De Vos, W., M., Wang, J., Li, J., Doré, J., Dusko Ehrlich, S., Stamatakis, A., & Bork, P. Nature Methods, 10(12):1196-1199, 2013.
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Paper doi abstract bibtex
Paper doi abstract bibtex To quantify known and unknown microorganisms at species-level resolution using shotgun sequencing data, we developed a method that establishes metagenomic operational taxonomic units (mOTUs) based on single-copy phylogenetic marker genes. Applied to 252 human fecal samples, the method revealed that on average 43% of the species abundance and 58% of the richness cannot be captured by current reference genome-based methods. An implementation of the method is available at http://www.bork.embl.de/software/mOTU/. © 2013 Nature America, Inc.
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title = {Metagenomic species profiling using universal phylogenetic marker genes},
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year = {2013},
pages = {1196-1199},
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abstract = {To quantify known and unknown microorganisms at species-level resolution using shotgun sequencing data, we developed a method that establishes metagenomic operational taxonomic units (mOTUs) based on single-copy phylogenetic marker genes. Applied to 252 human fecal samples, the method revealed that on average 43% of the species abundance and 58% of the richness cannot be captured by current reference genome-based methods. An implementation of the method is available at http://www.bork.embl.de/software/mOTU/. © 2013 Nature America, Inc.},
bibtype = {article},
author = {Sunagawa, Shinichi and Mende, Daniel R. and Zeller, Georg and Izquierdo-Carrasco, Fernando and Berger, Simon A. and Kultima, Jens Roat and Coelho, Luis Pedro and Arumugam, Manimozhiyan and Tap, Julien and Nielsen, Henrik Bjørn and Rasmussen, Simon and Brunak, Søren and Pedersen, Oluf and Guarner, Francisco and De Vos, Willem M. and Wang, Jun and Li, Junhua and Doré, Joël and Dusko Ehrlich, S. and Stamatakis, Alexandros and Bork, Peer},
doi = {10.1038/nmeth.2693},
journal = {Nature Methods},
number = {12}
}Downloads: 0
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