Template-dependent biosynthesis of poly(G)·poly(C) and its antiviral activity in vitro and in vivo

Template-dependent biosynthesis of poly(G)·poly(C) and its antiviral activity in vitro and in vivo. Surzhik, M., Vilner, L., Katchurin, A., & Timkovskii, A. Antiviral Research, 38(2):131-140, 1998. cited By 1

Paper doi abstract bibtex

Experimental conditions for poly(G) synthesis from GTP on a poly(C) template with the aid of Escherichia coli DNA-dependent RNA polymerase were investigated. The reaction product was purified without the use of RNase. On the basis of spectral data, gel permeation chromatography, affinity adsorption and electron microscopic visualization, the poly(G)·poly(C) product was assumed to possess a high degree of structural regularity. Its in vitro and in vivo antiviral activities were compared with those of traditional poly(G)·poly(C) and poly(I)·poly(C). Template-dependent poly(G)·poly(C was similar in its in vitro activity to poly(I)·poly(C) or even surpassed it, whereas the 'traditional' poly(G)·poly(C) was only slightly active in vitro. However, 'traditional poly(G)·poly(C) and poly(I)·poly(C) had similar activity in vivo, whereas template-dependent poly(G)·poly(C) was much less active in vivo. The role of intramolecular structural regularity in the in vitro and in vivo antiviral activity of polyribonucleotide duplexes is discussed.

@ARTICLE{Surzhik1998131,
author={Surzhik, M.A. and Vilner, L.M. and Katchurin, A.L. and Timkovskii, A.L.},
title={Template-dependent biosynthesis of poly(G)·poly(C) and its antiviral activity in vitro and in vivo},
journal={Antiviral Research},
year={1998},
volume={38},
number={2},
pages={131-140},
doi={10.1016/S0166-3542(98)00018-7},
note={cited By 1},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031816538&doi=10.1016%2fS0166-3542%2898%2900018-7&partnerID=40&md5=03d8b4a10254ed0aaf6c11191377a550},
affiliation={Laboratory of Biopolymers, Div. Molec. Radiat. Biophys., P., Leningrad District, 188 350, Russian Federation; Inst. Poliomyelitis Viral E., Russian Academy of Medical Sciences, Moscow District, 142 782, Russian Federation},
abstract={Experimental conditions for poly(G) synthesis from GTP on a poly(C) template with the aid of Escherichia coli DNA-dependent RNA polymerase were investigated. The reaction product was purified without the use of RNase. On the basis of spectral data, gel permeation chromatography, affinity adsorption and electron microscopic visualization, the poly(G)·poly(C) product was assumed to possess a high degree of structural regularity. Its in vitro and in vivo antiviral activities were compared with those of traditional poly(G)·poly(C) and poly(I)·poly(C). Template-dependent poly(G)·poly(C was similar in its in vitro activity to poly(I)·poly(C) or even surpassed it, whereas the 'traditional' poly(G)·poly(C) was only slightly active in vitro. However, 'traditional poly(G)·poly(C) and poly(I)·poly(C) had similar activity in vivo, whereas template-dependent poly(G)·poly(C) was much less active in vivo. The role of intramolecular structural regularity in the in vitro and in vivo antiviral activity of polyribonucleotide duplexes is discussed.},
author_keywords={Antiviral activity;  In vitro;  Poly(G)·poly(C);  Polyribonucleotide duplexes;  Structure;  Template biosynthesis},
correspondence_address1={Timkovskii, A.L.; Laboratory of Biopolymers, Molecular/Radiation Biophysics Div., Petersburg Nuclear Physics Institute, Gatchina, Leningrad District 188350, Russian Federation; email: ALT@virus.iem.ras.spb.ru},
issn={01663542},
coden={ARSRD},
pubmed_id={9707375},
language={English},
abbrev_source_title={Antiviral Res.},
document_type={Article},
source={Scopus},
}

Downloads: 0

{"_id":"7SKjwsevmetQb4YR3","bibbaseid":"surzhik-vilner-katchurin-timkovskii-templatedependentbiosynthesisofpolygpolycanditsantiviralactivityinvitroandinvivo-1998","authorIDs":[],"author_short":["Surzhik, M.","Vilner, L.","Katchurin, A.","Timkovskii, A."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Surzhik"],"firstnames":["M.A."],"suffixes":[]},{"propositions":[],"lastnames":["Vilner"],"firstnames":["L.M."],"suffixes":[]},{"propositions":[],"lastnames":["Katchurin"],"firstnames":["A.L."],"suffixes":[]},{"propositions":[],"lastnames":["Timkovskii"],"firstnames":["A.L."],"suffixes":[]}],"title":"Template-dependent biosynthesis of poly(G)·poly(C) and its antiviral activity in vitro and in vivo","journal":"Antiviral Research","year":"1998","volume":"38","number":"2","pages":"131-140","doi":"10.1016/S0166-3542(98)00018-7","note":"cited By 1","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031816538&doi=10.1016%2fS0166-3542%2898%2900018-7&partnerID=40&md5=03d8b4a10254ed0aaf6c11191377a550","affiliation":"Laboratory of Biopolymers, Div. Molec. Radiat. Biophys., P., Leningrad District, 188 350, Russian Federation; Inst. Poliomyelitis Viral E., Russian Academy of Medical Sciences, Moscow District, 142 782, Russian Federation","abstract":"Experimental conditions for poly(G) synthesis from GTP on a poly(C) template with the aid of Escherichia coli DNA-dependent RNA polymerase were investigated. The reaction product was purified without the use of RNase. On the basis of spectral data, gel permeation chromatography, affinity adsorption and electron microscopic visualization, the poly(G)·poly(C) product was assumed to possess a high degree of structural regularity. Its in vitro and in vivo antiviral activities were compared with those of traditional poly(G)·poly(C) and poly(I)·poly(C). Template-dependent poly(G)·poly(C was similar in its in vitro activity to poly(I)·poly(C) or even surpassed it, whereas the 'traditional' poly(G)·poly(C) was only slightly active in vitro. However, 'traditional poly(G)·poly(C) and poly(I)·poly(C) had similar activity in vivo, whereas template-dependent poly(G)·poly(C) was much less active in vivo. The role of intramolecular structural regularity in the in vitro and in vivo antiviral activity of polyribonucleotide duplexes is discussed.","author_keywords":"Antiviral activity; In vitro; Poly(G)·poly(C); Polyribonucleotide duplexes; Structure; Template biosynthesis","correspondence_address1":"Timkovskii, A.L.; Laboratory of Biopolymers, Molecular/Radiation Biophysics Div., Petersburg Nuclear Physics Institute, Gatchina, Leningrad District 188350, Russian Federation; email: ALT@virus.iem.ras.spb.ru","issn":"01663542","coden":"ARSRD","pubmed_id":"9707375","language":"English","abbrev_source_title":"Antiviral Res.","document_type":"Article","source":"Scopus","bibtex":"@ARTICLE{Surzhik1998131,\r\nauthor={Surzhik, M.A. and Vilner, L.M. and Katchurin, A.L. and Timkovskii, A.L.},\r\ntitle={Template-dependent biosynthesis of poly(G)·poly(C) and its antiviral activity in vitro and in vivo},\r\njournal={Antiviral Research},\r\nyear={1998},\r\nvolume={38},\r\nnumber={2},\r\npages={131-140},\r\ndoi={10.1016/S0166-3542(98)00018-7},\r\nnote={cited By 1},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031816538&doi=10.1016%2fS0166-3542%2898%2900018-7&partnerID=40&md5=03d8b4a10254ed0aaf6c11191377a550},\r\naffiliation={Laboratory of Biopolymers, Div. Molec. Radiat. Biophys., P., Leningrad District, 188 350, Russian Federation; Inst. Poliomyelitis Viral E., Russian Academy of Medical Sciences, Moscow District, 142 782, Russian Federation},\r\nabstract={Experimental conditions for poly(G) synthesis from GTP on a poly(C) template with the aid of Escherichia coli DNA-dependent RNA polymerase were investigated. The reaction product was purified without the use of RNase. On the basis of spectral data, gel permeation chromatography, affinity adsorption and electron microscopic visualization, the poly(G)·poly(C) product was assumed to possess a high degree of structural regularity. Its in vitro and in vivo antiviral activities were compared with those of traditional poly(G)·poly(C) and poly(I)·poly(C). Template-dependent poly(G)·poly(C was similar in its in vitro activity to poly(I)·poly(C) or even surpassed it, whereas the 'traditional' poly(G)·poly(C) was only slightly active in vitro. However, 'traditional poly(G)·poly(C) and poly(I)·poly(C) had similar activity in vivo, whereas template-dependent poly(G)·poly(C) was much less active in vivo. The role of intramolecular structural regularity in the in vitro and in vivo antiviral activity of polyribonucleotide duplexes is discussed.},\r\nauthor_keywords={Antiviral activity; In vitro; Poly(G)·poly(C); Polyribonucleotide duplexes; Structure; Template biosynthesis},\r\ncorrespondence_address1={Timkovskii, A.L.; Laboratory of Biopolymers, Molecular/Radiation Biophysics Div., Petersburg Nuclear Physics Institute, Gatchina, Leningrad District 188350, Russian Federation; email: ALT@virus.iem.ras.spb.ru},\r\nissn={01663542},\r\ncoden={ARSRD},\r\npubmed_id={9707375},\r\nlanguage={English},\r\nabbrev_source_title={Antiviral Res.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n","author_short":["Surzhik, M.","Vilner, L.","Katchurin, A.","Timkovskii, A."],"key":"Surzhik1998131","id":"Surzhik1998131","bibbaseid":"surzhik-vilner-katchurin-timkovskii-templatedependentbiosynthesisofpolygpolycanditsantiviralactivityinvitroandinvivo-1998","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031816538&doi=10.1016%2fS0166-3542%2898%2900018-7&partnerID=40&md5=03d8b4a10254ed0aaf6c11191377a550"},"metadata":{"authorlinks":{}},"downloads":0},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lmkb_2019_10.txt","creationDate":"2019-11-05T14:32:09.852Z","downloads":0,"keywords":[],"search_terms":["template","dependent","biosynthesis","poly","poly","antiviral","activity","vitro","vivo","surzhik","vilner","katchurin","timkovskii"],"title":"Template-dependent biosynthesis of poly(G)·poly(C) and its antiviral activity in vitro and in vivo","year":1998,"dataSources":["rgjRz8LhiuL9Prc4S"]}