@article{Swarbrick2020,
abstract = {MR1-restricted T (MR1T) cells are defined by their recognition of metabolite antigens presented by the monomorphic MHC class 1-related molecule, MR1, the most highly conserved MHC class I related molecule in mammalian species. Mucosal-associated invariant T (MAIT) cells are the predominant subset of MR1T cells expressing an invariant TCR $\alpha$-chain, TRAV1-2. These cells comprise a T cell subset that recognizes and mediates host immune responses to a broad array of microbial pathogens, including Mycobacterium tuberculosis. Here, we sought to characterize development of circulating human MR1T cells as defined by MR1-5-OP-RU tetramer labeling and of the TRAV1-2 + MAIT cells defined by expression of TRAV1-2 and high expression of CD26 and CD161 (TRAV1-2 + CD161 ++ CD26 ++ cells). We analyzed postnatal expansion, maturation, and functionality of peripheral blood MR1-5-OP-RU tetramer + MR1T cells in cohorts from three different geographic settings with different tuberculosis (TB) vaccination practices, levels of exposure to and infection with M. tuberculosis. Early after birth, frequencies of MR1-5-OP-RU tetramer + MR1T cells increased rapidly by several fold. This coincided with the transition from a predominantly CD4 + and TRAV1-2 − population in neonates, to a predominantly TRAV1-2 + CD161 ++ CD26 ++ CD8 + population. We also observed that tetramer + MR1T cells that expressed TNF upon mycobacterial stimulation were very low in neonates, but increased ∼10-fold in the first year of life. These functional MR1T cells in all age groups were MR1-5-OP-RU tetramer + TRAV1-2 + and highly expressed CD161 and CD26, markers that appeared to signal phenotypic and functional maturation of this cell subset. This age-associated maturation was also marked by the loss of na{\"{i}}ve T cell markers on tetramer + TRAV1-2 + MR1T cells more rapidly than tetramer + TRAV1-2 − MR1T cells and non-MR1T cells. These data suggest that neonates have infrequent populations of MR1T cells with diverse phenotypic attributes; Swarbrick et al. Human MR1 T Cell Development and that exposure to the environment rapidly and preferentially expands the MR1-5-OP-RU tetramer + TRAV1-2 + population of MR1T cells, which becomes the predominant population of functional MR1T cells early during childhood.},
author = {Swarbrick, Gwendolyn M. and Gela, Anele and Cansler, Meghan E. and Null, Megan D. and Duncan, Rowan B. and Nemes, Elisa and Shey, Muki and Nsereko, Mary and Mayanja-Kizza, Harriet and Kiguli, Sarah and Koh, Jeffrey and Hanekom, Willem A. and Hatherill, Mark and Lancioni, Christina and Lewinsohn, David M. and Scriba, Thomas J. and Lewinsohn, Deborah A.},
doi = {10.3389/fimmu.2020.556695},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Swarbrick et al. - 2020 - Postnatal expansion, maturation, and functionality of MR1T cells in humans.pdf:pdf},
issn = {1664-3224},
journal = {Frontiers in Immunology},
keywords = {MAIT cells,OA,fund{\_}not{\_}ack,human mucosal immunology,infant,innate T cells,original,tuberculosis},
mendeley-tags = {OA,fund{\_}not{\_}ack,original},
month = {sep},
pages = {556695},
pmid = {33042140},
publisher = {Frontiers Media SA},
title = {{Postnatal expansion, maturation, and functionality of MR1T cells in humans}},
url = {https://www.frontiersin.org/article/10.3389/fimmu.2020.556695/full},
volume = {11},
year = {2020}
}

{"_id":"ga3gsQSmTiHJKA7rf","bibbaseid":"swarbrick-gela-cansler-null-duncan-nemes-shey-nsereko-etal-postnatalexpansionmaturationandfunctionalityofmr1tcellsinhumans-2020","authorIDs":[],"author_short":["Swarbrick, G. M.","Gela, A.","Cansler, M. E.","Null, M. D.","Duncan, R. B.","Nemes, E.","Shey, M.","Nsereko, M.","Mayanja-Kizza, H.","Kiguli, S.","Koh, J.","Hanekom, W. A.","Hatherill, M.","Lancioni, C.","Lewinsohn, D. M.","Scriba, T. J.","Lewinsohn, D. A."],"bibdata":{"bibtype":"article","type":"article","abstract":"MR1-restricted T (MR1T) cells are defined by their recognition of metabolite antigens presented by the monomorphic MHC class 1-related molecule, MR1, the most highly conserved MHC class I related molecule in mammalian species. Mucosal-associated invariant T (MAIT) cells are the predominant subset of MR1T cells expressing an invariant TCR $α$-chain, TRAV1-2. These cells comprise a T cell subset that recognizes and mediates host immune responses to a broad array of microbial pathogens, including Mycobacterium tuberculosis. Here, we sought to characterize development of circulating human MR1T cells as defined by MR1-5-OP-RU tetramer labeling and of the TRAV1-2 + MAIT cells defined by expression of TRAV1-2 and high expression of CD26 and CD161 (TRAV1-2 + CD161 ++ CD26 ++ cells). We analyzed postnatal expansion, maturation, and functionality of peripheral blood MR1-5-OP-RU tetramer + MR1T cells in cohorts from three different geographic settings with different tuberculosis (TB) vaccination practices, levels of exposure to and infection with M. tuberculosis. Early after birth, frequencies of MR1-5-OP-RU tetramer + MR1T cells increased rapidly by several fold. This coincided with the transition from a predominantly CD4 + and TRAV1-2 − population in neonates, to a predominantly TRAV1-2 + CD161 ++ CD26 ++ CD8 + population. We also observed that tetramer + MR1T cells that expressed TNF upon mycobacterial stimulation were very low in neonates, but increased ∼10-fold in the first year of life. These functional MR1T cells in all age groups were MR1-5-OP-RU tetramer + TRAV1-2 + and highly expressed CD161 and CD26, markers that appeared to signal phenotypic and functional maturation of this cell subset. This age-associated maturation was also marked by the loss of naïve T cell markers on tetramer + TRAV1-2 + MR1T cells more rapidly than tetramer + TRAV1-2 − MR1T cells and non-MR1T cells. These data suggest that neonates have infrequent populations of MR1T cells with diverse phenotypic attributes; Swarbrick et al. Human MR1 T Cell Development and that exposure to the environment rapidly and preferentially expands the MR1-5-OP-RU tetramer + TRAV1-2 + population of MR1T cells, which becomes the predominant population of functional MR1T cells early during childhood.","author":[{"propositions":[],"lastnames":["Swarbrick"],"firstnames":["Gwendolyn","M."],"suffixes":[]},{"propositions":[],"lastnames":["Gela"],"firstnames":["Anele"],"suffixes":[]},{"propositions":[],"lastnames":["Cansler"],"firstnames":["Meghan","E."],"suffixes":[]},{"propositions":[],"lastnames":["Null"],"firstnames":["Megan","D."],"suffixes":[]},{"propositions":[],"lastnames":["Duncan"],"firstnames":["Rowan","B."],"suffixes":[]},{"propositions":[],"lastnames":["Nemes"],"firstnames":["Elisa"],"suffixes":[]},{"propositions":[],"lastnames":["Shey"],"firstnames":["Muki"],"suffixes":[]},{"propositions":[],"lastnames":["Nsereko"],"firstnames":["Mary"],"suffixes":[]},{"propositions":[],"lastnames":["Mayanja-Kizza"],"firstnames":["Harriet"],"suffixes":[]},{"propositions":[],"lastnames":["Kiguli"],"firstnames":["Sarah"],"suffixes":[]},{"propositions":[],"lastnames":["Koh"],"firstnames":["Jeffrey"],"suffixes":[]},{"propositions":[],"lastnames":["Hanekom"],"firstnames":["Willem","A."],"suffixes":[]},{"propositions":[],"lastnames":["Hatherill"],"firstnames":["Mark"],"suffixes":[]},{"propositions":[],"lastnames":["Lancioni"],"firstnames":["Christina"],"suffixes":[]},{"propositions":[],"lastnames":["Lewinsohn"],"firstnames":["David","M."],"suffixes":[]},{"propositions":[],"lastnames":["Scriba"],"firstnames":["Thomas","J."],"suffixes":[]},{"propositions":[],"lastnames":["Lewinsohn"],"firstnames":["Deborah","A."],"suffixes":[]}],"doi":"10.3389/fimmu.2020.556695","file":":C$\\$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Swarbrick et al. - 2020 - Postnatal expansion, maturation, and functionality of MR1T cells in humans.pdf:pdf","issn":"1664-3224","journal":"Frontiers in Immunology","keywords":"MAIT cells,OA,fund_not_ack,human mucosal immunology,infant,innate T cells,original,tuberculosis","mendeley-tags":"OA,fund_not_ack,original","month":"sep","pages":"556695","pmid":"33042140","publisher":"Frontiers Media SA","title":"Postnatal expansion, maturation, and functionality of MR1T cells in humans","url":"https://www.frontiersin.org/article/10.3389/fimmu.2020.556695/full","volume":"11","year":"2020","bibtex":"@article{Swarbrick2020,\r\nabstract = {MR1-restricted T (MR1T) cells are defined by their recognition of metabolite antigens presented by the monomorphic MHC class 1-related molecule, MR1, the most highly conserved MHC class I related molecule in mammalian species. Mucosal-associated invariant T (MAIT) cells are the predominant subset of MR1T cells expressing an invariant TCR $\\alpha$-chain, TRAV1-2. These cells comprise a T cell subset that recognizes and mediates host immune responses to a broad array of microbial pathogens, including Mycobacterium tuberculosis. Here, we sought to characterize development of circulating human MR1T cells as defined by MR1-5-OP-RU tetramer labeling and of the TRAV1-2 + MAIT cells defined by expression of TRAV1-2 and high expression of CD26 and CD161 (TRAV1-2 + CD161 ++ CD26 ++ cells). We analyzed postnatal expansion, maturation, and functionality of peripheral blood MR1-5-OP-RU tetramer + MR1T cells in cohorts from three different geographic settings with different tuberculosis (TB) vaccination practices, levels of exposure to and infection with M. tuberculosis. Early after birth, frequencies of MR1-5-OP-RU tetramer + MR1T cells increased rapidly by several fold. This coincided with the transition from a predominantly CD4 + and TRAV1-2 − population in neonates, to a predominantly TRAV1-2 + CD161 ++ CD26 ++ CD8 + population. We also observed that tetramer + MR1T cells that expressed TNF upon mycobacterial stimulation were very low in neonates, but increased ∼10-fold in the first year of life. These functional MR1T cells in all age groups were MR1-5-OP-RU tetramer + TRAV1-2 + and highly expressed CD161 and CD26, markers that appeared to signal phenotypic and functional maturation of this cell subset. This age-associated maturation was also marked by the loss of na{\\\"{i}}ve T cell markers on tetramer + TRAV1-2 + MR1T cells more rapidly than tetramer + TRAV1-2 − MR1T cells and non-MR1T cells. These data suggest that neonates have infrequent populations of MR1T cells with diverse phenotypic attributes; Swarbrick et al. Human MR1 T Cell Development and that exposure to the environment rapidly and preferentially expands the MR1-5-OP-RU tetramer + TRAV1-2 + population of MR1T cells, which becomes the predominant population of functional MR1T cells early during childhood.},\r\nauthor = {Swarbrick, Gwendolyn M. and Gela, Anele and Cansler, Meghan E. and Null, Megan D. and Duncan, Rowan B. and Nemes, Elisa and Shey, Muki and Nsereko, Mary and Mayanja-Kizza, Harriet and Kiguli, Sarah and Koh, Jeffrey and Hanekom, Willem A. and Hatherill, Mark and Lancioni, Christina and Lewinsohn, David M. and Scriba, Thomas J. and Lewinsohn, Deborah A.},\r\ndoi = {10.3389/fimmu.2020.556695},\r\nfile = {:C$\\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Swarbrick et al. - 2020 - Postnatal expansion, maturation, and functionality of MR1T cells in humans.pdf:pdf},\r\nissn = {1664-3224},\r\njournal = {Frontiers in Immunology},\r\nkeywords = {MAIT cells,OA,fund{\\_}not{\\_}ack,human mucosal immunology,infant,innate T cells,original,tuberculosis},\r\nmendeley-tags = {OA,fund{\\_}not{\\_}ack,original},\r\nmonth = {sep},\r\npages = {556695},\r\npmid = {33042140},\r\npublisher = {Frontiers Media SA},\r\ntitle = {{Postnatal expansion, maturation, and functionality of MR1T cells in humans}},\r\nurl = {https://www.frontiersin.org/article/10.3389/fimmu.2020.556695/full},\r\nvolume = {11},\r\nyear = {2020}\r\n}\r\n","author_short":["Swarbrick, G. M.","Gela, A.","Cansler, M. E.","Null, M. D.","Duncan, R. B.","Nemes, E.","Shey, M.","Nsereko, M.","Mayanja-Kizza, H.","Kiguli, S.","Koh, J.","Hanekom, W. A.","Hatherill, M.","Lancioni, C.","Lewinsohn, D. M.","Scriba, T. J.","Lewinsohn, D. A."],"key":"Swarbrick2020","id":"Swarbrick2020","bibbaseid":"swarbrick-gela-cansler-null-duncan-nemes-shey-nsereko-etal-postnatalexpansionmaturationandfunctionalityofmr1tcellsinhumans-2020","role":"author","urls":{"Paper":"https://www.frontiersin.org/article/10.3389/fimmu.2020.556695/full"},"keyword":["MAIT cells","OA","fund_not_ack","human mucosal immunology","infant","innate T cells","original","tuberculosis"],"metadata":{"authorlinks":{}},"downloads":0},"bibtype":"article","biburl":"https://drive.google.com/uc?export=download&id=1-JLqZ7RwZ3VC2d6ErLGHAtOeMRS_7GCz","creationDate":"2020-11-06T03:32:23.212Z","downloads":0,"keywords":["mait cells","oa","fund_not_ack","human mucosal immunology","infant","innate t cells","original","tuberculosis"],"search_terms":["postnatal","expansion","maturation","functionality","mr1t","cells","humans","swarbrick","gela","cansler","null","duncan","nemes","shey","nsereko","mayanja-kizza","kiguli","koh","hanekom","hatherill","lancioni","lewinsohn","scriba","lewinsohn"],"title":"Postnatal expansion, maturation, and functionality of MR1T cells in humans","year":2020,"dataSources":["Krmt6gt9ktB2s6ARh","FdCBAxFeyKEmJd8WK","9bX4N36CTXtCXNFMd","wrEvssexmuYudwQw9"]}