@article{RN176,
   author = {Sweeney, D. C. and Reberšek, M. and Dermol, J. and Rems, L. and Miklavčič, D. and Davalos, R. V.},
   title = {Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses},
   journal = {Biochim Biophys Acta},
   volume = {1858},
   number = {11},
   pages = {2689-2698},
   note = {Sweeney, Daniel C
Reberšek, Matej
Dermol, Janja
Rems, Lea
Miklavčič, Damijan
Davalos, Rafael V
Journal Article
Research Support, Non-U.S. Gov't
Netherlands
2016/07/04
Biochim Biophys Acta. 2016 Nov;1858(11):2689-2698. doi: 10.1016/j.bbamem.2016.06.024. Epub 2016 Jun 29.},
   abstract = {High-frequency bipolar electric pulses have been shown to mitigate undesirable muscle contraction during irreversible electroporation (IRE) therapy. Here, we evaluate the potential applicability of such pulses for introducing exogenous molecules into cells, such as in electrochemotherapy (ECT). For this purpose we develop a method for calculating the time course of the effective permeability of an electroporated cell membrane based on real-time imaging of propidium transport into single cells that allows a quantitative comparison between different pulsing schemes. We calculate the effective permeability for several pulsed electric field treatments including trains of 100μs monopolar pulses, conventionally used in IRE and ECT, and pulse trains containing bursts or evenly-spaced 1μs bipolar pulses. We show that shorter bipolar pulses induce lower effective membrane permeability than longer monopolar pulses with equivalent treatment times. This lower efficiency can be attributed to incomplete membrane charging. Nevertheless, bipolar pulses could be used for increasing the uptake of small molecules into cells more symmetrically, but at the expense of higher applied voltages. These data indicate that high-frequency bipolar bursts of electrical pulses may be designed to electroporate cells as effectively as and more homogeneously than conventional monopolar pulses.},
   keywords = {Animals
Biological Transport
CHO Cells
Cell Membrane/*metabolism
Cell Membrane Permeability
Cricetulus
Electrodes
Electroporation/*methods
Membrane Potentials/physiology
Propidium/*metabolism
Single-Cell Analysis/instrumentation/*methods
Bipolar electrical pulses
Electrochemotherapy
Electroporation
Gene electrotransfer
Irreversible electroporation
Permeability},
   ISSN = {0006-3002 (Print)
0006-3002},
   DOI = {10.1016/j.bbamem.2016.06.024},
   year = {2016},
   type = {Journal Article}
}

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V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Sweeney"],"firstnames":["D.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Reberšek"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Dermol"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Rems"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Miklavčič"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses","journal":"Biochim Biophys Acta","volume":"1858","number":"11","pages":"2689-2698","note":"Sweeney, Daniel C Reberšek, Matej Dermol, Janja Rems, Lea Miklavčič, Damijan Davalos, Rafael V Journal Article Research Support, Non-U.S. Gov't Netherlands 2016/07/04 Biochim Biophys Acta. 2016 Nov;1858(11):2689-2698. doi: 10.1016/j.bbamem.2016.06.024. Epub 2016 Jun 29.","abstract":"High-frequency bipolar electric pulses have been shown to mitigate undesirable muscle contraction during irreversible electroporation (IRE) therapy. Here, we evaluate the potential applicability of such pulses for introducing exogenous molecules into cells, such as in electrochemotherapy (ECT). For this purpose we develop a method for calculating the time course of the effective permeability of an electroporated cell membrane based on real-time imaging of propidium transport into single cells that allows a quantitative comparison between different pulsing schemes. We calculate the effective permeability for several pulsed electric field treatments including trains of 100μs monopolar pulses, conventionally used in IRE and ECT, and pulse trains containing bursts or evenly-spaced 1μs bipolar pulses. We show that shorter bipolar pulses induce lower effective membrane permeability than longer monopolar pulses with equivalent treatment times. This lower efficiency can be attributed to incomplete membrane charging. Nevertheless, bipolar pulses could be used for increasing the uptake of small molecules into cells more symmetrically, but at the expense of higher applied voltages. These data indicate that high-frequency bipolar bursts of electrical pulses may be designed to electroporate cells as effectively as and more homogeneously than conventional monopolar pulses.","keywords":"Animals Biological Transport CHO Cells Cell Membrane/*metabolism Cell Membrane Permeability Cricetulus Electrodes Electroporation/*methods Membrane Potentials/physiology Propidium/*metabolism Single-Cell Analysis/instrumentation/*methods Bipolar electrical pulses Electrochemotherapy Electroporation Gene electrotransfer Irreversible electroporation Permeability","issn":"0006-3002 (Print) 0006-3002","doi":"10.1016/j.bbamem.2016.06.024","year":"2016","bibtex":"@article{RN176,\n author = {Sweeney, D. C. and Reberšek, M. and Dermol, J. and Rems, L. and Miklavčič, D. and Davalos, R. V.},\n title = {Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses},\n journal = {Biochim Biophys Acta},\n volume = {1858},\n number = {11},\n pages = {2689-2698},\n note = {Sweeney, Daniel C\nReberšek, Matej\nDermol, Janja\nRems, Lea\nMiklavčič, Damijan\nDavalos, Rafael V\nJournal Article\nResearch Support, Non-U.S. Gov't\nNetherlands\n2016/07/04\nBiochim Biophys Acta. 2016 Nov;1858(11):2689-2698. doi: 10.1016/j.bbamem.2016.06.024. Epub 2016 Jun 29.},\n abstract = {High-frequency bipolar electric pulses have been shown to mitigate undesirable muscle contraction during irreversible electroporation (IRE) therapy. Here, we evaluate the potential applicability of such pulses for introducing exogenous molecules into cells, such as in electrochemotherapy (ECT). For this purpose we develop a method for calculating the time course of the effective permeability of an electroporated cell membrane based on real-time imaging of propidium transport into single cells that allows a quantitative comparison between different pulsing schemes. We calculate the effective permeability for several pulsed electric field treatments including trains of 100μs monopolar pulses, conventionally used in IRE and ECT, and pulse trains containing bursts or evenly-spaced 1μs bipolar pulses. We show that shorter bipolar pulses induce lower effective membrane permeability than longer monopolar pulses with equivalent treatment times. This lower efficiency can be attributed to incomplete membrane charging. Nevertheless, bipolar pulses could be used for increasing the uptake of small molecules into cells more symmetrically, but at the expense of higher applied voltages. These data indicate that high-frequency bipolar bursts of electrical pulses may be designed to electroporate cells as effectively as and more homogeneously than conventional monopolar pulses.},\n keywords = {Animals\nBiological Transport\nCHO Cells\nCell Membrane/*metabolism\nCell Membrane Permeability\nCricetulus\nElectrodes\nElectroporation/*methods\nMembrane Potentials/physiology\nPropidium/*metabolism\nSingle-Cell Analysis/instrumentation/*methods\nBipolar electrical pulses\nElectrochemotherapy\nElectroporation\nGene electrotransfer\nIrreversible electroporation\nPermeability},\n ISSN = {0006-3002 (Print)\n0006-3002},\n DOI = {10.1016/j.bbamem.2016.06.024},\n year = {2016},\n type = {Journal Article}\n}\n\n","author_short":["Sweeney, D. C.","Reberšek, M.","Dermol, J.","Rems, L.","Miklavčič, D.","Davalos, R. V."],"key":"RN176","id":"RN176","bibbaseid":"sweeney-reberek-dermol-rems-miklavi-davalos-quantificationofcellmembranepermeabilityinducedbymonopolarandhighfrequencybipolarburstsofelectricalpulses-2016","role":"author","urls":{},"keyword":["Animals Biological Transport CHO Cells Cell Membrane/*metabolism Cell Membrane Permeability Cricetulus Electrodes Electroporation/*methods Membrane Potentials/physiology Propidium/*metabolism Single-Cell Analysis/instrumentation/*methods Bipolar electrical pulses Electrochemotherapy Electroporation Gene electrotransfer Irreversible electroporation Permeability"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["animals biological transport cho cells cell membrane/*metabolism cell membrane permeability cricetulus electrodes electroporation/*methods membrane potentials/physiology propidium/*metabolism single-cell analysis/instrumentation/*methods bipolar electrical pulses electrochemotherapy electroporation gene electrotransfer irreversible electroporation permeability"],"search_terms":["quantification","cell","membrane","permeability","induced","monopolar","high","frequency","bipolar","bursts","electrical","pulses","sweeney","reberšek","dermol","rems","miklavčič","davalos"],"title":"Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses","year":2016}