@article{syed_three-dimensional_2017,
	title = {Three-{Dimensional} {Imaging} of {Transparent} {Tissues} via {Metal} {Nanoparticle} {Labeling}},
	volume = {139},
	issn = {0002-7863},
	url = {https://doi.org/10.1021/jacs.7b04022},
	doi = {10.1021/jacs.7b04022},
	abstract = {Chemical probes are key components of the bioimaging toolbox, as they label biomolecules in cells and tissues. The new challenge in bioimaging is to design chemical probes for three-dimensional (3D) tissue imaging. In this work, we discovered that light scattering of metal nanoparticles can provide 3D imaging contrast in intact and transparent tissues. The nanoparticles can act as a template for the chemical growth of a metal layer to further enhance the scattering signal. The use of chemically grown nanoparticles in whole tissues can amplify the scattering to produce a 1.4 million-fold greater photon yield than obtained using common fluorophores. These probes are non-photobleaching and can be used alongside fluorophores without interference. We demonstrated three distinct biomedical applications: (a) molecular imaging of blood vessels, (b) tracking of nanodrug carriers in tumors, and (c) mapping of lesions and immune cells in a multiple sclerosis mouse model. Our strategy establishes a distinct yet complementary set of imaging probes for understanding disease mechanisms in three dimensions.},
	number = {29},
	urldate = {2021-11-06},
	journal = {J. Am. Chem. Soc.},
	author = {Syed, Abdullah Muhammad and Sindhwani, Shrey and Wilhelm, Stefan and Kingston, Benjamin R. and Lee, Dennis S. W. and Gommerman, Jennifer L. and Chan, Warren C. W.},
	month = jul,
	year = {2017},
	note = {Publisher: American Chemical Society},
	pages = {9961--9971},
	file = {Full Text PDF:files/1886/Syed et al. - 2017 - Three-Dimensional Imaging of Transparent Tissues v.pdf:application/pdf;ACS Full Text Snapshot:files/1888/jacs.html:text/html},
	url_Paper = {https://inbs.med.utoronto.ca/wp-content/uploads/2022/01/Syed-et-al.-2017-Three-Dimensional-Imaging-of-Transparent-Tissues-v.pdf}
}

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The nanoparticles can act as a template for the chemical growth of a metal layer to further enhance the scattering signal. The use of chemically grown nanoparticles in whole tissues can amplify the scattering to produce a 1.4 million-fold greater photon yield than obtained using common fluorophores. These probes are non-photobleaching and can be used alongside fluorophores without interference. We demonstrated three distinct biomedical applications: (a) molecular imaging of blood vessels, (b) tracking of nanodrug carriers in tumors, and (c) mapping of lesions and immune cells in a multiple sclerosis mouse model. Our strategy establishes a distinct yet complementary set of imaging probes for understanding disease mechanisms in three dimensions.","number":"29","urldate":"2021-11-06","journal":"J. Am. Chem. 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