@article{
 title = {Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and a Semiclosed Greenhouses .1},
 type = {article},
 year = {1994},
 pages = {101-105},
 volume = {44},
 websites = {<Go to ISI>://A1994NQ11700019},
 id = {53bf7a02-cd58-3a5a-9acb-b699a4ce9077},
 created = {2012-01-05T13:09:19.000Z},
 file_attached = {false},
 profile_id = {1a467167-0a41-3583-a6a3-034c31031332},
 group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},
 last_modified = {2012-01-05T13:15:12.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 source_type = {Journal Article},
 abstract = {Environmental risk evaluation of transgenic melon plants introduced with coat protein gene of cucumber mosaic virus was carried out in a closed and a semiclosed greenhouse. The following evaluation items were; compared between transgenic melon plants and non-transgenic melon plants. 1. Morphological characteristics was compared in a closed greenhouse. Fruits maturation period was examined in a semi-closed greenhouse. Morphological characteristics of transgenic melon plants were not different from those of non-transgenic melon plants. Maturation period of the transgenic melon plants was 44.7 days in average and the non-transgenic melon plants was 43.0 days in average. This result demonstrated that transgenic melon plants did not differ from the non-transgenic plants in fruit maturation period. 2. Pollen form and fertility were not different between transgenic and non-transgenic melon plants. Longevity of the pollen from transgenic and non-transgenic melon were examined on fine days in May 1992. Pollen was collected at 9 : 30, 11 : 30, 13 : 30, 15 : 30, 17 : 30 from both transgenic and non-transgenic melon plants, then sowed onto pollen germination medium. Most of the pollen collected at 9 : 30 germinated. Few collected at 13 : 30 germinated, and pollen collected at 15 : 30 did not germinate. Therefore, longevity of the pollen in both transgenic and non-transgenic melon plants seems to be within about 13 : 30 in a closed greenhouse on fine day. 3. Wind pollination of transgenic and non-transgenic melon plants were investigated under artificial wind, generated by an electric fan, in a closed greenhouse. Pollen germination medium were placed at various distance, 0, 5, 10, 15, 50, 100, 200 and 300 cm from the plants. The wind was blown from 10 : 00 to 15 : 30, at a velocity of 0.5-4.0 m/sec. Pollen from transgenic or non-transgenic melon plants were not detected on germination medium at all distance from the plants. We concluded that pollen of melon, an entmophilous plant, dose not disperse by wind, and is generally dispersed only by insects. 4. Seed fertilities of transgenic and non- transgenic plants were 75.5 % and 58.4 %, respectively. We considered that this difference was due to the affection of weakness of non-transgenic seedling. Germination ratios of seeds stored for 0, 6 and 12 months were not different between transgenic and non-transgenic melon. 5. Cross compatibility of melon (Cucumis melo) in Cucurbitaceae was investigated. Pollen of melon were pollinated to female flowers of cucumber, C. angria, C. metuliferus, C. africanus, watermelon, pumpkin, bottle gourd and bitter gourd. Twenty-four hrs after pollination, elongation of pollen tubes in the styles was investigated by staining with aniline blue. Degrees of elongation of pollen tubes were dependent on the female plants. However, the pollen from the non-transgenic melon plants did not fertilize any related species. These results indicated that melon can not fertilize other species than melon. From these results, it is reveled that there was no difference between transgenic melon and non-transgenic melon plants for characteristics of morphology and gene flow.},
 bibtype = {article},
 author = {Tabei, Y and Oosawa, K and Nishimura, S and Hanada, K and Yoshioka, K and Fujisawa, I and Nakajima, K},
 journal = {Breeding Science},
 number = {1}
}

{"_id":"2GeXq8qD3ZajvybPY","bibbaseid":"tabei-oosawa-nishimura-hanada-yoshioka-fujisawa-nakajima-environmentalriskevaluationofthetransgenicmelonwithcoatproteingeneofcucumbermosaicvirusinaclosedandasemiclosedgreenhouses1-1994","downloads":0,"creationDate":"2015-08-05T17:19:28.369Z","title":"Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and a Semiclosed Greenhouses .1","author_short":["Tabei, Y.","Oosawa, K.","Nishimura, S.","Hanada, K.","Yoshioka, K.","Fujisawa, I.","Nakajima, K."],"year":1994,"bibtype":"article","biburl":null,"bibdata":{"title":"Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and a Semiclosed Greenhouses .1","type":"article","year":"1994","pages":"101-105","volume":"44","websites":"<Go to ISI>://A1994NQ11700019","id":"53bf7a02-cd58-3a5a-9acb-b699a4ce9077","created":"2012-01-05T13:09:19.000Z","file_attached":false,"profile_id":"1a467167-0a41-3583-a6a3-034c31031332","group_id":"0e532975-1a47-38a4-ace8-4fe5968bcd72","last_modified":"2012-01-05T13:15:12.000Z","read":false,"starred":false,"authored":false,"confirmed":"true","hidden":false,"source_type":"Journal Article","abstract":"Environmental risk evaluation of transgenic melon plants introduced with coat protein gene of cucumber mosaic virus was carried out in a closed and a semiclosed greenhouse. The following evaluation items were; compared between transgenic melon plants and non-transgenic melon plants. 1. Morphological characteristics was compared in a closed greenhouse. Fruits maturation period was examined in a semi-closed greenhouse. Morphological characteristics of transgenic melon plants were not different from those of non-transgenic melon plants. Maturation period of the transgenic melon plants was 44.7 days in average and the non-transgenic melon plants was 43.0 days in average. This result demonstrated that transgenic melon plants did not differ from the non-transgenic plants in fruit maturation period. 2. Pollen form and fertility were not different between transgenic and non-transgenic melon plants. Longevity of the pollen from transgenic and non-transgenic melon were examined on fine days in May 1992. Pollen was collected at 9 : 30, 11 : 30, 13 : 30, 15 : 30, 17 : 30 from both transgenic and non-transgenic melon plants, then sowed onto pollen germination medium. Most of the pollen collected at 9 : 30 germinated. Few collected at 13 : 30 germinated, and pollen collected at 15 : 30 did not germinate. Therefore, longevity of the pollen in both transgenic and non-transgenic melon plants seems to be within about 13 : 30 in a closed greenhouse on fine day. 3. Wind pollination of transgenic and non-transgenic melon plants were investigated under artificial wind, generated by an electric fan, in a closed greenhouse. Pollen germination medium were placed at various distance, 0, 5, 10, 15, 50, 100, 200 and 300 cm from the plants. The wind was blown from 10 : 00 to 15 : 30, at a velocity of 0.5-4.0 m/sec. Pollen from transgenic or non-transgenic melon plants were not detected on germination medium at all distance from the plants. We concluded that pollen of melon, an entmophilous plant, dose not disperse by wind, and is generally dispersed only by insects. 4. Seed fertilities of transgenic and non- transgenic plants were 75.5 % and 58.4 %, respectively. We considered that this difference was due to the affection of weakness of non-transgenic seedling. Germination ratios of seeds stored for 0, 6 and 12 months were not different between transgenic and non-transgenic melon. 5. Cross compatibility of melon (Cucumis melo) in Cucurbitaceae was investigated. Pollen of melon were pollinated to female flowers of cucumber, C. angria, C. metuliferus, C. africanus, watermelon, pumpkin, bottle gourd and bitter gourd. Twenty-four hrs after pollination, elongation of pollen tubes in the styles was investigated by staining with aniline blue. Degrees of elongation of pollen tubes were dependent on the female plants. However, the pollen from the non-transgenic melon plants did not fertilize any related species. These results indicated that melon can not fertilize other species than melon. From these results, it is reveled that there was no difference between transgenic melon and non-transgenic melon plants for characteristics of morphology and gene flow.","bibtype":"article","author":"Tabei, Y and Oosawa, K and Nishimura, S and Hanada, K and Yoshioka, K and Fujisawa, I and Nakajima, K","journal":"Breeding Science","number":"1","bibtex":"@article{\n title = {Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and a Semiclosed Greenhouses .1},\n type = {article},\n year = {1994},\n pages = {101-105},\n volume = {44},\n websites = {<Go to ISI>://A1994NQ11700019},\n id = {53bf7a02-cd58-3a5a-9acb-b699a4ce9077},\n created = {2012-01-05T13:09:19.000Z},\n file_attached = {false},\n profile_id = {1a467167-0a41-3583-a6a3-034c31031332},\n group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},\n last_modified = {2012-01-05T13:15:12.000Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n abstract = {Environmental risk evaluation of transgenic melon plants introduced with coat protein gene of cucumber mosaic virus was carried out in a closed and a semiclosed greenhouse. The following evaluation items were; compared between transgenic melon plants and non-transgenic melon plants. 1. Morphological characteristics was compared in a closed greenhouse. Fruits maturation period was examined in a semi-closed greenhouse. Morphological characteristics of transgenic melon plants were not different from those of non-transgenic melon plants. Maturation period of the transgenic melon plants was 44.7 days in average and the non-transgenic melon plants was 43.0 days in average. This result demonstrated that transgenic melon plants did not differ from the non-transgenic plants in fruit maturation period. 2. Pollen form and fertility were not different between transgenic and non-transgenic melon plants. Longevity of the pollen from transgenic and non-transgenic melon were examined on fine days in May 1992. Pollen was collected at 9 : 30, 11 : 30, 13 : 30, 15 : 30, 17 : 30 from both transgenic and non-transgenic melon plants, then sowed onto pollen germination medium. Most of the pollen collected at 9 : 30 germinated. Few collected at 13 : 30 germinated, and pollen collected at 15 : 30 did not germinate. Therefore, longevity of the pollen in both transgenic and non-transgenic melon plants seems to be within about 13 : 30 in a closed greenhouse on fine day. 3. Wind pollination of transgenic and non-transgenic melon plants were investigated under artificial wind, generated by an electric fan, in a closed greenhouse. Pollen germination medium were placed at various distance, 0, 5, 10, 15, 50, 100, 200 and 300 cm from the plants. The wind was blown from 10 : 00 to 15 : 30, at a velocity of 0.5-4.0 m/sec. Pollen from transgenic or non-transgenic melon plants were not detected on germination medium at all distance from the plants. We concluded that pollen of melon, an entmophilous plant, dose not disperse by wind, and is generally dispersed only by insects. 4. Seed fertilities of transgenic and non- transgenic plants were 75.5 % and 58.4 %, respectively. We considered that this difference was due to the affection of weakness of non-transgenic seedling. Germination ratios of seeds stored for 0, 6 and 12 months were not different between transgenic and non-transgenic melon. 5. Cross compatibility of melon (Cucumis melo) in Cucurbitaceae was investigated. Pollen of melon were pollinated to female flowers of cucumber, C. angria, C. metuliferus, C. africanus, watermelon, pumpkin, bottle gourd and bitter gourd. Twenty-four hrs after pollination, elongation of pollen tubes in the styles was investigated by staining with aniline blue. Degrees of elongation of pollen tubes were dependent on the female plants. However, the pollen from the non-transgenic melon plants did not fertilize any related species. These results indicated that melon can not fertilize other species than melon. From these results, it is reveled that there was no difference between transgenic melon and non-transgenic melon plants for characteristics of morphology and gene flow.},\n bibtype = {article},\n author = {Tabei, Y and Oosawa, K and Nishimura, S and Hanada, K and Yoshioka, K and Fujisawa, I and Nakajima, K},\n journal = {Breeding Science},\n number = {1}\n}","author_short":["Tabei, Y.","Oosawa, K.","Nishimura, S.","Hanada, K.","Yoshioka, K.","Fujisawa, I.","Nakajima, K."],"bibbaseid":"tabei-oosawa-nishimura-hanada-yoshioka-fujisawa-nakajima-environmentalriskevaluationofthetransgenicmelonwithcoatproteingeneofcucumbermosaicvirusinaclosedandasemiclosedgreenhouses1-1994","role":"author","urls":{},"downloads":0,"html":""},"search_terms":["environmental","risk","evaluation","transgenic","melon","coat","protein","gene","cucumber","mosaic","virus","closed","semiclosed","greenhouses","tabei","oosawa","nishimura","hanada","yoshioka","fujisawa","nakajima"],"keywords":[],"authorIDs":[]}