@article{
 title = {Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and Semiclosed Greenhouse .2},
 type = {article},
 year = {1994},
 pages = {207-211},
 volume = {44},
 websites = {<Go to ISI>://A1994NV85000015},
 id = {f03dc412-f519-39f4-b940-4f073ce91cb2},
 created = {2012-01-05T13:09:19.000Z},
 file_attached = {false},
 profile_id = {1a467167-0a41-3583-a6a3-034c31031332},
 group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},
 last_modified = {2012-01-05T13:15:11.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 source_type = {Journal Article},
 abstract = {Environmental risk evaluation of transgenic melon plants introduced with the coat protein gene of cucumber mosaic virus was carried out in a closed and a semi-closed greenhouse as described in previous studies (Tabei et al. 1994). 1. The possibility of harmful influences on environment due to compounds produced by transgenic melon were examined. The following compounds were compared between the transgenic melon and non-transgenic melon plants: (1) phenolic acids, generally considered as allelochemical substances, produced in the plant body and secreted from the root, and (2) production of volatile compounds released from the plant into the atmosphere. Germination ratio, root length and fresh weight of cabbage in the soil used for the cultivation of either transgenic or non- transgenic melon plants, and in the soil mixed with dry powder prepared from these respective melon plants. Specific phenolic acids and volatile compounds were not detected from transgenic melon plants. There were no differences for germination ratio, root length and fresh weight of cabbage between transgenic and non-transgenic melon. These results suggested that transgenic melon plants did not produce any specific products influencing environment and other plants. 2.The influence of transgenic melon cultivation on the soil microflora was investigated in a semi-closed greenhouse. Transgenic melon and non-transgenic melon plants were cultivated in pots filled with unsterilized soil. After cultivation, the number of microbes, bacteria, actinomycetes and fungi, in the soil was determined. The number of actinomycetes and fungi in the soil cultivated with transgenic melon was slightly larger than the soil cultivated with non-transgenic melon plants. However these differences were not significant, we concluded that the effect of transgenic melon plants on microflora was not different from non-transgenic melon plants. 3. Agrobacterium tumefaciens strain LBA 4404 was used as a vector for production of the transgenic melon. Residue of this bacterium on/in plant body was examined in a closed greenhouse. Microorganisms were isolated by shaking or homogenizing the transgenic melon and non-transgenic melon plants in sterile distilled water and plated on YEB media containing certain selective antibiotics. Strain LBA 4404 of A. tumefaciens was not detected on the surface or in tissues of the plants. Form these results, it is suggested that there are no differences between transgenic melon and non-transgenic melon for harmful impact to other plants and soil microflora. Moreover strain LBA 4404 was not detected on the surface or in tissues of the plants. In conclusion, together with the previous study, the influence of a transgenic melon on the environment was not different from that of a non-transgenic melon within the experiments of a closed and a semi-closed greenhouse. In addition, experiments and cultivation of transgenic melon plants were safely carried out throughout the experiments in the a closed and a semi- closed greenhouse.},
 bibtype = {article},
 author = {Tabei, Y and Oosawa, K and Nishimura, S and Watanabe, S and Tsuchiya, K and Yoshioka, K and Fujisawa, I and Nakajima, K},
 journal = {Breeding Science},
 number = {2}
}

{"_id":"D4ZF5TP3maTckydyJ","bibbaseid":"tabei-oosawa-nishimura-watanabe-tsuchiya-yoshioka-fujisawa-nakajima-environmentalriskevaluationofthetransgenicmelonwithcoatproteingeneofcucumbermosaicvirusinaclosedandsemiclosedgreenhouse2-1994","downloads":0,"creationDate":"2015-08-05T17:19:28.325Z","title":"Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and Semiclosed Greenhouse .2","author_short":["Tabei, Y.","Oosawa, K.","Nishimura, S.","Watanabe, S.","Tsuchiya, K.","Yoshioka, K.","Fujisawa, I.","Nakajima, K."],"year":1994,"bibtype":"article","biburl":null,"bibdata":{"title":"Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and Semiclosed Greenhouse .2","type":"article","year":"1994","pages":"207-211","volume":"44","websites":"<Go to ISI>://A1994NV85000015","id":"f03dc412-f519-39f4-b940-4f073ce91cb2","created":"2012-01-05T13:09:19.000Z","file_attached":false,"profile_id":"1a467167-0a41-3583-a6a3-034c31031332","group_id":"0e532975-1a47-38a4-ace8-4fe5968bcd72","last_modified":"2012-01-05T13:15:11.000Z","read":false,"starred":false,"authored":false,"confirmed":"true","hidden":false,"source_type":"Journal Article","abstract":"Environmental risk evaluation of transgenic melon plants introduced with the coat protein gene of cucumber mosaic virus was carried out in a closed and a semi-closed greenhouse as described in previous studies (Tabei et al. 1994). 1. The possibility of harmful influences on environment due to compounds produced by transgenic melon were examined. The following compounds were compared between the transgenic melon and non-transgenic melon plants: (1) phenolic acids, generally considered as allelochemical substances, produced in the plant body and secreted from the root, and (2) production of volatile compounds released from the plant into the atmosphere. Germination ratio, root length and fresh weight of cabbage in the soil used for the cultivation of either transgenic or non- transgenic melon plants, and in the soil mixed with dry powder prepared from these respective melon plants. Specific phenolic acids and volatile compounds were not detected from transgenic melon plants. There were no differences for germination ratio, root length and fresh weight of cabbage between transgenic and non-transgenic melon. These results suggested that transgenic melon plants did not produce any specific products influencing environment and other plants. 2.The influence of transgenic melon cultivation on the soil microflora was investigated in a semi-closed greenhouse. Transgenic melon and non-transgenic melon plants were cultivated in pots filled with unsterilized soil. After cultivation, the number of microbes, bacteria, actinomycetes and fungi, in the soil was determined. The number of actinomycetes and fungi in the soil cultivated with transgenic melon was slightly larger than the soil cultivated with non-transgenic melon plants. However these differences were not significant, we concluded that the effect of transgenic melon plants on microflora was not different from non-transgenic melon plants. 3. Agrobacterium tumefaciens strain LBA 4404 was used as a vector for production of the transgenic melon. Residue of this bacterium on/in plant body was examined in a closed greenhouse. Microorganisms were isolated by shaking or homogenizing the transgenic melon and non-transgenic melon plants in sterile distilled water and plated on YEB media containing certain selective antibiotics. Strain LBA 4404 of A. tumefaciens was not detected on the surface or in tissues of the plants. Form these results, it is suggested that there are no differences between transgenic melon and non-transgenic melon for harmful impact to other plants and soil microflora. Moreover strain LBA 4404 was not detected on the surface or in tissues of the plants. In conclusion, together with the previous study, the influence of a transgenic melon on the environment was not different from that of a non-transgenic melon within the experiments of a closed and a semi-closed greenhouse. In addition, experiments and cultivation of transgenic melon plants were safely carried out throughout the experiments in the a closed and a semi- closed greenhouse.","bibtype":"article","author":"Tabei, Y and Oosawa, K and Nishimura, S and Watanabe, S and Tsuchiya, K and Yoshioka, K and Fujisawa, I and Nakajima, K","journal":"Breeding Science","number":"2","bibtex":"@article{\n title = {Environmental Risk-Evaluation of the Transgenic Melon with Coat Protein Gene of Cucumber Mosaic-Virus in a Closed and Semiclosed Greenhouse .2},\n type = {article},\n year = {1994},\n pages = {207-211},\n volume = {44},\n websites = {<Go to ISI>://A1994NV85000015},\n id = {f03dc412-f519-39f4-b940-4f073ce91cb2},\n created = {2012-01-05T13:09:19.000Z},\n file_attached = {false},\n profile_id = {1a467167-0a41-3583-a6a3-034c31031332},\n group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},\n last_modified = {2012-01-05T13:15:11.000Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n source_type = {Journal Article},\n abstract = {Environmental risk evaluation of transgenic melon plants introduced with the coat protein gene of cucumber mosaic virus was carried out in a closed and a semi-closed greenhouse as described in previous studies (Tabei et al. 1994). 1. The possibility of harmful influences on environment due to compounds produced by transgenic melon were examined. The following compounds were compared between the transgenic melon and non-transgenic melon plants: (1) phenolic acids, generally considered as allelochemical substances, produced in the plant body and secreted from the root, and (2) production of volatile compounds released from the plant into the atmosphere. Germination ratio, root length and fresh weight of cabbage in the soil used for the cultivation of either transgenic or non- transgenic melon plants, and in the soil mixed with dry powder prepared from these respective melon plants. Specific phenolic acids and volatile compounds were not detected from transgenic melon plants. There were no differences for germination ratio, root length and fresh weight of cabbage between transgenic and non-transgenic melon. These results suggested that transgenic melon plants did not produce any specific products influencing environment and other plants. 2.The influence of transgenic melon cultivation on the soil microflora was investigated in a semi-closed greenhouse. Transgenic melon and non-transgenic melon plants were cultivated in pots filled with unsterilized soil. After cultivation, the number of microbes, bacteria, actinomycetes and fungi, in the soil was determined. The number of actinomycetes and fungi in the soil cultivated with transgenic melon was slightly larger than the soil cultivated with non-transgenic melon plants. However these differences were not significant, we concluded that the effect of transgenic melon plants on microflora was not different from non-transgenic melon plants. 3. Agrobacterium tumefaciens strain LBA 4404 was used as a vector for production of the transgenic melon. Residue of this bacterium on/in plant body was examined in a closed greenhouse. Microorganisms were isolated by shaking or homogenizing the transgenic melon and non-transgenic melon plants in sterile distilled water and plated on YEB media containing certain selective antibiotics. Strain LBA 4404 of A. tumefaciens was not detected on the surface or in tissues of the plants. Form these results, it is suggested that there are no differences between transgenic melon and non-transgenic melon for harmful impact to other plants and soil microflora. Moreover strain LBA 4404 was not detected on the surface or in tissues of the plants. In conclusion, together with the previous study, the influence of a transgenic melon on the environment was not different from that of a non-transgenic melon within the experiments of a closed and a semi-closed greenhouse. In addition, experiments and cultivation of transgenic melon plants were safely carried out throughout the experiments in the a closed and a semi- closed greenhouse.},\n bibtype = {article},\n author = {Tabei, Y and Oosawa, K and Nishimura, S and Watanabe, S and Tsuchiya, K and Yoshioka, K and Fujisawa, I and Nakajima, K},\n journal = {Breeding Science},\n number = {2}\n}","author_short":["Tabei, Y.","Oosawa, K.","Nishimura, S.","Watanabe, S.","Tsuchiya, K.","Yoshioka, K.","Fujisawa, I.","Nakajima, K."],"bibbaseid":"tabei-oosawa-nishimura-watanabe-tsuchiya-yoshioka-fujisawa-nakajima-environmentalriskevaluationofthetransgenicmelonwithcoatproteingeneofcucumbermosaicvirusinaclosedandsemiclosedgreenhouse2-1994","role":"author","urls":{},"downloads":0,"html":""},"search_terms":["environmental","risk","evaluation","transgenic","melon","coat","protein","gene","cucumber","mosaic","virus","closed","semiclosed","greenhouse","tabei","oosawa","nishimura","watanabe","tsuchiya","yoshioka","fujisawa","nakajima"],"keywords":[],"authorIDs":[]}