Targeted lipidomics approach for endogenous N-acyl amino acids in rat brain tissue. Tan, B., Yu, Y. W., Monn, M. F., Hughes, H. V., O'Dell, D. K., & Walker, J. M. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877(26):2890–2894, September, 2009. Place: Netherlandsdoi abstract bibtex Great effort has been devoted to characterize signaling lipids in central nervous system. This has led to a search for novel strategies to characterize hitherto unknown lipid compositions. Here we developed two methods, one for identification and one for quantification, for N-acyl amino acids, a novel lipid family. The identification method contains a series of purification steps followed by nano-LC/MS/MS and high-throughput screening of the datasets with a potent search algorithm based on fragment ion analysis. MS/MS spectra with good quality can be obtained with 150 fmol of targeted lipids on column with our nano-LC/MS/MS. More than one thousand mass spectra generated using the information dependent acquisition mode of Analyst QS software can be analyzed in 1 min using our home built software. The quantification method utilized the multiple reaction monitoring mode in Analyst software to measure the endogenous levels of N-acyl amino acids in rat brain. Using these two methods we were able to identify and quantify 11 previously reported N-acyl amino acids with endogenous levels ranging from 0.26 to 333 pmol g(-1) wet rat brain.
@article{tan_targeted_2009,
title = {Targeted lipidomics approach for endogenous {N}-acyl amino acids in rat brain tissue.},
volume = {877},
issn = {1873-376X 1570-0232},
doi = {10.1016/j.jchromb.2009.01.002},
abstract = {Great effort has been devoted to characterize signaling lipids in central nervous system. This has led to a search for novel strategies to characterize hitherto unknown lipid compositions. Here we developed two methods, one for identification and one for quantification, for N-acyl amino acids, a novel lipid family. The identification method contains a series of purification steps followed by nano-LC/MS/MS and high-throughput screening of the datasets with a potent search algorithm based on fragment ion analysis. MS/MS spectra with good quality can be obtained with 150 fmol of targeted lipids on column with our nano-LC/MS/MS. More than one thousand mass spectra generated using the information dependent acquisition mode of Analyst QS software can be analyzed in 1 min using our home built software. The quantification method utilized the multiple reaction monitoring mode in Analyst software to measure the endogenous levels of N-acyl amino acids in rat brain. Using these two methods we were able to identify and quantify 11 previously reported N-acyl amino acids with endogenous levels ranging from 0.26 to 333 pmol g(-1) wet rat brain.},
language = {eng},
number = {26},
journal = {Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
author = {Tan, Bo and Yu, Y. William and Monn, M. Francesca and Hughes, H. Velocity and O'Dell, David K. and Walker, J. Michael},
month = sep,
year = {2009},
pmid = {19168403},
note = {Place: Netherlands},
keywords = {*Brain Chemistry, Amino Acids/*chemistry, Animals, Chromatography, Liquid/*methods, Lipids/*chemistry, Male, Rats, Rats, Sprague-Dawley, Software, Tandem Mass Spectrometry/*methods},
pages = {2890--2894},
}
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