p15 Expression Differentiates Nevus from Melanoma. Taylor, L. A., O'Day, C., Dentchev, T., Hood, K., Chu, E. Y., Ridky, T. W., & Seykora, J. T. The American journal of pathology, 186(12):3094–3099, December, 2016. Number: 12doi abstract bibtex Most melanomas are driven by BRAF(V600E)-activating mutations, while nevi harboring the same mutations have growth arrest. Although decreased p16 expression has been associated with melanoma formation, in recent work, p15 represented a primary effector of oncogene-induced senescence in nevomelanocytes that was diminished in melanomas. This study determined whether decreased p15 levels represent a general biomarker for the transition from nevus to melanoma. We performed p15 and p16 IHC analyses on a random series of nevi and melanomas. Staining was evaluated and graded for percentage and intensity to determine the H score. For real-time quantitative RT-PCR analysis of p15, RNA was extracted from FFPE sections from 14 nevus and melanoma samples via macrodissection. A two-sided t-test was used to evaluate between-group differences in mean H scores and qDeltaCt values. p15 Expression was significantly increased in melanocytic nevi compared with melanomas (mean H scores, 254.8 versus 132.3; P \textless 0.001). On p15 staining, the H score differential was greater than that with p16 staining [122.5 (P \textless 0.001) and 64.8 (P = 0.055), respectively]. Real-time quantitative RT-PCR analysis revealed a lower mean qDeltaCt value in melanomas, consistent with lower p15 expression (P = 0.018). Together, these data support the hypothesis that decreased p15 expression is a robust biomarker for distinguishing nevus from melanoma.
@article{taylor_p15_2016,
title = {p15 {Expression} {Differentiates} {Nevus} from {Melanoma}},
volume = {186},
doi = {S0002-9440(16)30363-7 [pii]},
abstract = {Most melanomas are driven by BRAF(V600E)-activating mutations, while nevi harboring the same mutations have growth arrest. Although decreased p16 expression has been associated with melanoma formation, in recent work, p15 represented a primary effector of oncogene-induced senescence in nevomelanocytes that was diminished in melanomas. This study determined whether decreased p15 levels represent a general biomarker for the transition from nevus to melanoma. We performed p15 and p16 IHC analyses on a random series of nevi and melanomas. Staining was evaluated and graded for percentage and intensity to determine the H score. For real-time quantitative RT-PCR analysis of p15, RNA was extracted from FFPE sections from 14 nevus and melanoma samples via macrodissection. A two-sided t-test was used to evaluate between-group differences in mean H scores and qDeltaCt values. p15 Expression was significantly increased in melanocytic nevi compared with melanomas (mean H scores, 254.8 versus 132.3; P {\textless} 0.001). On p15 staining, the H score differential was greater than that with p16 staining [122.5 (P {\textless} 0.001) and 64.8 (P = 0.055), respectively]. Real-time quantitative RT-PCR analysis revealed a lower mean qDeltaCt value in melanomas, consistent with lower p15 expression (P = 0.018). Together, these data support the hypothesis that decreased p15 expression is a robust biomarker for distinguishing nevus from melanoma.},
language = {eng},
number = {12},
journal = {The American journal of pathology},
author = {Taylor, L. A. and O'Day, C. and Dentchev, T. and Hood, K. and Chu, E. Y. and Ridky, T. W. and Seykora, J. T.},
month = dec,
year = {2016},
pmcid = {PMC5225287. CPAT Core (A).},
pmid = {27855847},
note = {Number: 12},
keywords = {CPAT Core},
pages = {3094--3099},
}
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Staining was evaluated and graded for percentage and intensity to determine the H score. For real-time quantitative RT-PCR analysis of p15, RNA was extracted from FFPE sections from 14 nevus and melanoma samples via macrodissection. A two-sided t-test was used to evaluate between-group differences in mean H scores and qDeltaCt values. p15 Expression was significantly increased in melanocytic nevi compared with melanomas (mean H scores, 254.8 versus 132.3; P \\textless 0.001). On p15 staining, the H score differential was greater than that with p16 staining [122.5 (P \\textless 0.001) and 64.8 (P = 0.055), respectively]. Real-time quantitative RT-PCR analysis revealed a lower mean qDeltaCt value in melanomas, consistent with lower p15 expression (P = 0.018). 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