Characterization of Yersinia enterocolitica strains potentially virulent for humans and animals in river water. Terech-Majewska, E., Pajdak, J., Platt-Samoraj, A., Szczerba-Turek, A., Bancerz-Kisiel, A., & Grabowska, K. Journal of Applied Microbiology, 121(2):554 – 560, 2016. Cited by: 9![Characterization of Yersinia enterocolitica strains potentially virulent for humans and animals in river water [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Aims: The aim of this study was to isolate and identify potentially pathogenic strains of Yersinia enterocolitica in water samples collected from the upstream section of the Drwęca River in Poland. Methods and Results: Thirty-nine water samples were collected. Strains were isolated, identified with the use of the API®20E test kit (Biomerieux, Marcy l'Etoile, France) at 37°C, serotyped and subjected to a molecular analysis. Multiplex PCR was carried out to amplify three virulence genes: ail, ystA and ystB. Fragments of ail and ystA genes were not identified in the genetic material of the analysed strains. The ystB gene was identified in four strains. Yersinia enterocolitica strains of biotype 1A, which contain the ystB gene, may cause gastrointestinal problems. Conclusions: In our study, Y. enterocolitica strains of biotype 1A/ystB with serotypes 0 : 3, 0 : 5 and 0 : 8 were identified in samples collected from the Drwęca River which flows through the areas protected by Natura 2000, one of the largest networks of nature conservation areas in the European Union. The presence of Y. enterocolitica in the Drwęca River indicates that the analysed bacteria colonize natural water bodies. Significance and Impact of the Study: Most research focuses on food or sewage as a source of Y. enterocolitica infections. Little is known about the occurrence of this pathogen in natural waters. Our results show that natural waters are also a potential threat to human and animal health. © 2016 The Society for Applied Microbiology
@ARTICLE{Terech-Majewska2016554,
author = {Terech-Majewska, E. and Pajdak, J. and Platt-Samoraj, A. and Szczerba-Turek, A. and Bancerz-Kisiel, A. and Grabowska, K.},
title = {Characterization of Yersinia enterocolitica strains potentially virulent for humans and animals in river water},
year = {2016},
journal = {Journal of Applied Microbiology},
volume = {121},
number = {2},
pages = {554 – 560},
doi = {10.1111/jam.13182},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978496225&doi=10.1111%2fjam.13182&partnerID=40&md5=33c9ffe5763058e3572ec6228e0adeba},
affiliations = {Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland},
abstract = {Aims: The aim of this study was to isolate and identify potentially pathogenic strains of Yersinia enterocolitica in water samples collected from the upstream section of the Drwęca River in Poland. Methods and Results: Thirty-nine water samples were collected. Strains were isolated, identified with the use of the API®20E test kit (Biomerieux, Marcy l'Etoile, France) at 37°C, serotyped and subjected to a molecular analysis. Multiplex PCR was carried out to amplify three virulence genes: ail, ystA and ystB. Fragments of ail and ystA genes were not identified in the genetic material of the analysed strains. The ystB gene was identified in four strains. Yersinia enterocolitica strains of biotype 1A, which contain the ystB gene, may cause gastrointestinal problems. Conclusions: In our study, Y. enterocolitica strains of biotype 1A/ystB with serotypes 0 : 3, 0 : 5 and 0 : 8 were identified in samples collected from the Drwęca River which flows through the areas protected by Natura 2000, one of the largest networks of nature conservation areas in the European Union. The presence of Y. enterocolitica in the Drwęca River indicates that the analysed bacteria colonize natural water bodies. Significance and Impact of the Study: Most research focuses on food or sewage as a source of Y. enterocolitica infections. Little is known about the occurrence of this pathogen in natural waters. Our results show that natural waters are also a potential threat to human and animal health. © 2016 The Society for Applied Microbiology},
author_keywords = {PCR; reservoir; water; Yersinia enterocolitica; ystB gene},
keywords = {Drweca River; Poland [Central Europe]; Animalia; Yersinia enterocolitica; Animals; Conservation; Genes; Health risks; Rivers; Sewage; river water; Molecular analysis; Natural waters; Pathogenic strains; PCR; River water; Test kits; Virulence gene; Water samples; Yersinia enterocolitica; Ystb gene; bacterium; European Union; gastroenteritis; gene expression; hominid; identification method; microbiology; molecular analysis; polymerase chain reaction; reservoir; river water; virulence; ail gene; Article; bacterial colonization; bacterial strain; bacterial virulence; bacterium isolation; controlled study; gastrointestinal symptom; gene identification; multiplex polymerase chain reaction; nonhuman; Poland; water sampling; Yersinia enterocolitica; ystA gene; ystB gene; Reservoirs (water)},
correspondence_address = {J. Pajdak; Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland; email: joanna.pajdak@uwm.edu.pl},
issn = {13645072},
coden = {JAMIF},
pmid = {27203356},
language = {English},
abbrev_source_title = {J. Appl. Microbiol.},
type = {Article},
publication_stage = {Final},
source = {Scopus},
note = {Cited by: 9}
}
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