Quantification of bound bicarbonate in photosystem II. Tikhonov, K., Shevela, D., Klimov, V. V., & Messinger, J. Photosynthetica, 56(1):210–216, March, 2018. Paper doi abstract bibtex In this study, we presented a new approach for quantification of bicarbonate (HCO3−) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and 18O-labelling, excludes the possibility of “non-accounted” HCO3− by avoiding (1) the employment of formate for removal of HCO3− from PSII, and (2) the extremely low concentrations of HCO3−/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3−, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 ± 0.1 HCO3− are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3− molecule as ligand to the non-heme iron of PSII, while unbound HCO3− optimizes the water-splitting reactions by acting as a mobile proton shuttle.
@article{tikhonov_quantification_2018,
title = {Quantification of bound bicarbonate in photosystem {II}},
volume = {56},
issn = {1573-9058},
url = {https://doi.org/10.1007/s11099-017-0758-4},
doi = {10.1007/s11099-017-0758-4},
abstract = {In this study, we presented a new approach for quantification of bicarbonate (HCO3−) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and 18O-labelling, excludes the possibility of “non-accounted” HCO3− by avoiding (1) the employment of formate for removal of HCO3− from PSII, and (2) the extremely low concentrations of HCO3−/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3−, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 ± 0.1 HCO3− are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3− molecule as ligand to the non-heme iron of PSII, while unbound HCO3− optimizes the water-splitting reactions by acting as a mobile proton shuttle.},
language = {en},
number = {1},
urldate = {2024-12-10},
journal = {Photosynthetica},
author = {Tikhonov, K. and Shevela, D. and Klimov, V. V. and Messinger, J.},
month = mar,
year = {2018},
keywords = {Mn-stabilizing protein, hydrogen carbonate, inorganic carbon, mass spectrometry, non-heme iron, oxygen-evolving complex},
pages = {210--216},
}
Downloads: 0
{"_id":"HtRnw9cH6updb5rx2","bibbaseid":"tikhonov-shevela-klimov-messinger-quantificationofboundbicarbonateinphotosystemii-2018","author_short":["Tikhonov, K.","Shevela, D.","Klimov, V. V.","Messinger, J."],"bibdata":{"bibtype":"article","type":"article","title":"Quantification of bound bicarbonate in photosystem II","volume":"56","issn":"1573-9058","url":"https://doi.org/10.1007/s11099-017-0758-4","doi":"10.1007/s11099-017-0758-4","abstract":"In this study, we presented a new approach for quantification of bicarbonate (HCO3−) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and 18O-labelling, excludes the possibility of “non-accounted” HCO3− by avoiding (1) the employment of formate for removal of HCO3− from PSII, and (2) the extremely low concentrations of HCO3−/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3−, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 ± 0.1 HCO3− are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3− molecule as ligand to the non-heme iron of PSII, while unbound HCO3− optimizes the water-splitting reactions by acting as a mobile proton shuttle.","language":"en","number":"1","urldate":"2024-12-10","journal":"Photosynthetica","author":[{"propositions":[],"lastnames":["Tikhonov"],"firstnames":["K."],"suffixes":[]},{"propositions":[],"lastnames":["Shevela"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Klimov"],"firstnames":["V.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Messinger"],"firstnames":["J."],"suffixes":[]}],"month":"March","year":"2018","keywords":"Mn-stabilizing protein, hydrogen carbonate, inorganic carbon, mass spectrometry, non-heme iron, oxygen-evolving complex","pages":"210–216","bibtex":"@article{tikhonov_quantification_2018,\n\ttitle = {Quantification of bound bicarbonate in photosystem {II}},\n\tvolume = {56},\n\tissn = {1573-9058},\n\turl = {https://doi.org/10.1007/s11099-017-0758-4},\n\tdoi = {10.1007/s11099-017-0758-4},\n\tabstract = {In this study, we presented a new approach for quantification of bicarbonate (HCO3−) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and 18O-labelling, excludes the possibility of “non-accounted” HCO3− by avoiding (1) the employment of formate for removal of HCO3− from PSII, and (2) the extremely low concentrations of HCO3−/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3−, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 ± 0.1 HCO3− are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3− molecule as ligand to the non-heme iron of PSII, while unbound HCO3− optimizes the water-splitting reactions by acting as a mobile proton shuttle.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2024-12-10},\n\tjournal = {Photosynthetica},\n\tauthor = {Tikhonov, K. and Shevela, D. and Klimov, V. V. and Messinger, J.},\n\tmonth = mar,\n\tyear = {2018},\n\tkeywords = {Mn-stabilizing protein, hydrogen carbonate, inorganic carbon, mass spectrometry, non-heme iron, oxygen-evolving complex},\n\tpages = {210--216},\n}\n\n\n\n","author_short":["Tikhonov, K.","Shevela, D.","Klimov, V. V.","Messinger, J."],"key":"tikhonov_quantification_2018","id":"tikhonov_quantification_2018","bibbaseid":"tikhonov-shevela-klimov-messinger-quantificationofboundbicarbonateinphotosystemii-2018","role":"author","urls":{"Paper":"https://doi.org/10.1007/s11099-017-0758-4"},"keyword":["Mn-stabilizing protein","hydrogen carbonate","inorganic carbon","mass spectrometry","non-heme iron","oxygen-evolving complex"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs"],"keywords":["mn-stabilizing protein","hydrogen carbonate","inorganic carbon","mass spectrometry","non-heme iron","oxygen-evolving complex"],"search_terms":["quantification","bound","bicarbonate","photosystem","tikhonov","shevela","klimov","messinger"],"title":"Quantification of bound bicarbonate in photosystem II","year":2018}