Applying nephelometry for analyzing liquid yeast cultures. Tünnermann, L., Colou, J., Näsholm, T., Löfstedt, T., & Gratz, R. Biochemistry and Biophysics Reports, 46:102572, June, 2026. ![Applying nephelometry for analyzing liquid yeast cultures [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Saccharomyces cerevisiae is a widely used model organism for the molecular analysis of genes and proteins. Several methods have been developed to study protein function and activity through heterologous gene expression, including yeast two-hybrid and yeast complementation. Traditionally, these yeast-based assays were performed on solid agar plates. While this approach provides an easy visual readout, it is difficult to quantify the results accurately. To overcome this limitation, liquid-based methods were introduced. Most of these methods rely on the use of spectrophotometry to measure reduction in light transmission as a result of light scattering and monitor culture growth. In this study, we propose nephelometry as an additional method for performing and analyzing liquid-culture yeast complementation assays. More specifically, we compare the suitability of using nephelometry for the functional analysis of two homologous proteins using yeast complementation: The amino acid transporter homologues Arabidopsis thaliana LYSINE HISTIDINE TRANSPORTER 1 (AtLHT1) and Populus tremula L. x tremuloides Michx LYSINE HISTIDINE TRANSPORTER 1.2 (PtrLHT1.2). In previous reports, no differences in microbial growth were detected, irrespective of which homolog was used to rescue an amino acid-deficient yeast mutant strain. By using nephelometry to record yeast growth, we demonstrated that it is a robust and reproducible method. When comparing to spectrophotometric measurements of yeast cultures, it proved to be a suitable alternative. The novel approach even revealed previously undetected differences in culture growth of both homologues, highlighting nephelometry's potential to improve sensitivity in yeast-based functional assays. We present the use of nephelometry as an equal method to yeast complementation traditionally executed on solid agar medium or in liquid culture with spectrophotometric analysis.
@article{tunnermann_applying_2026,
title = {Applying nephelometry for analyzing liquid yeast cultures},
volume = {46},
issn = {2405-5808},
url = {https://www.sciencedirect.com/science/article/pii/S2405580826001329},
doi = {10.1016/j.bbrep.2026.102572},
abstract = {Saccharomyces cerevisiae is a widely used model organism for the molecular analysis of genes and proteins. Several methods have been developed to study protein function and activity through heterologous gene expression, including yeast two-hybrid and yeast complementation. Traditionally, these yeast-based assays were performed on solid agar plates. While this approach provides an easy visual readout, it is difficult to quantify the results accurately. To overcome this limitation, liquid-based methods were introduced. Most of these methods rely on the use of spectrophotometry to measure reduction in light transmission as a result of light scattering and monitor culture growth. In this study, we propose nephelometry as an additional method for performing and analyzing liquid-culture yeast complementation assays. More specifically, we compare the suitability of using nephelometry for the functional analysis of two homologous proteins using yeast complementation: The amino acid transporter homologues Arabidopsis thaliana LYSINE HISTIDINE TRANSPORTER 1 (AtLHT1) and Populus tremula L. x tremuloides Michx LYSINE HISTIDINE TRANSPORTER 1.2 (PtrLHT1.2). In previous reports, no differences in microbial growth were detected, irrespective of which homolog was used to rescue an amino acid-deficient yeast mutant strain. By using nephelometry to record yeast growth, we demonstrated that it is a robust and reproducible method. When comparing to spectrophotometric measurements of yeast cultures, it proved to be a suitable alternative. The novel approach even revealed previously undetected differences in culture growth of both homologues, highlighting nephelometry's potential to improve sensitivity in yeast-based functional assays. We present the use of nephelometry as an equal method to yeast complementation traditionally executed on solid agar medium or in liquid culture with spectrophotometric analysis.},
urldate = {2026-04-17},
journal = {Biochemistry and Biophysics Reports},
author = {Tünnermann, Laura and Colou, Justine and Näsholm, Torgny and Löfstedt, Tommy and Gratz, Regina},
month = jun,
year = {2026},
keywords = {Lag time, Lysine histidine transporter 1 (LHT1), Maximum slope time, Nephelometry, Spectrophotometry},
pages = {102572},
}
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To overcome this limitation, liquid-based methods were introduced. Most of these methods rely on the use of spectrophotometry to measure reduction in light transmission as a result of light scattering and monitor culture growth. In this study, we propose nephelometry as an additional method for performing and analyzing liquid-culture yeast complementation assays. More specifically, we compare the suitability of using nephelometry for the functional analysis of two homologous proteins using yeast complementation: The amino acid transporter homologues Arabidopsis thaliana LYSINE HISTIDINE TRANSPORTER 1 (AtLHT1) and Populus tremula L. x tremuloides Michx LYSINE HISTIDINE TRANSPORTER 1.2 (PtrLHT1.2). In previous reports, no differences in microbial growth were detected, irrespective of which homolog was used to rescue an amino acid-deficient yeast mutant strain. By using nephelometry to record yeast growth, we demonstrated that it is a robust and reproducible method. 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