@article{Tominaga.2013, 
year = {01/01/2013}, 
rating = {3}, 
title = {{VSD Imaging Method of Ex Vivo Brain Preparation}}, 
author = {Tominaga, Takashi and Kajiwara, Riichi and Tominaga, Yoko}, 
journal = {Journal of Neuroscience and Neuroengineering}, 
issn = {2168-2011}, 
doi = {10.1166/jnsne.2013.1051}, 
url = {http://dx.doi.org/10.1166/jnsne.2013.1051}, 
abstract = {{Functional analysis of large-scale neuronal circuits using the voltage-sensitive dye (VSD) imaging method has become increasingly popular. Poor signal-to-noise ratio (SNR) is an unavoidable problem for the VSD imaging method. This review shows the relation between the SNR and the photon shot noise and baseline noise, and explains that the small sensitivity of VSD to membrane potential changes causes this problem. Based on this discussion, we will show the requirements for the experimental apparatus to work around these difficulties, focusing on the functional analysis of neural circuits in ex vivo specimen such as brain slice preparation and isolated whole brain preparation. These discussions will be useful for showing the current limitations and benefits of the VSD imaging method for analyzing the neuronal network activities of the brain.}}, 
number = {3}, 
volume = {2}, 
keywords = {}, 
local-url = {file://localhost/Users/tominaga/Documents/Papers%20Library/Tominaga-VSD%20Imaging%20Method%20of%20Ex%20Vivo%20Brain%20Preparation-2013-Journal%20of%20Neuroscience%20and%20Neuroengineering.pdf}
}

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