@article{RN144,
   author = {Trainito, C. I. and Sweeney, D. C. and Čemažar, J. and Schmelz, E. M. and Français, O. and Le Pioufle, B. and Davalos, R. V.},
   title = {Characterization of sequentially-staged cancer cells using electrorotation},
   journal = {PLoS One},
   volume = {14},
   number = {9},
   pages = {e0222289},
   note = {1932-6203
Trainito, Claudia I
Orcid: 0000-0002-2728-9364
Sweeney, Daniel C
Orcid: 0000-0002-1289-1627
Čemažar, Jaka
Schmelz, Eva M
Français, Olivier
Le Pioufle, Bruno
Davalos, Rafael V
R01 CA213423/CA/NCI NIH HHS/United States
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
United States
2019/09/20
PLoS One. 2019 Sep 19;14(9):e0222289. doi: 10.1371/journal.pone.0222289. eCollection 2019.},
   abstract = {The identification and separation of cells from heterogeneous populations is critical to the diagnosis of diseases. Label-free methodologies in particular have been developed to manipulate individual cells using properties such as density and morphology. The electrical properties of malignant cells, including the membrane capacitance and cytoplasmic conductivity, have been demonstrated to be altered compared to non-malignant cells of similar origin. Here, we exploit these changes to characterize individual cells in a sequentially-staged in vitro cancer model using electrorotation (EROT)-the rotation of a cell induced by a rotating electric field. Using a microfabricated device, a dielectrophoretic force to suspend cells while measuring their angular velocity resulting from an EROT force applied at frequencies between 3 kHz to 10 MHz. We experimentally determine the EROT response for cells at three stages of malignancy and analyze the resultant spectra by considering models that include the effect of the cell membrane alone (single-shell model) and the combined effect of the cell membrane and nucleus (double-shell model). We find that the cell membrane is largely responsible for a given cell's EROT response between 3 kHz and 10 MHz. Our results also indicate that membrane capacitance, membrane conductance, and cytoplasmic conductivity increase with an increasingly malignant phenotype. Our results demonstrate the potential of using electrorotation as a means making of non-invasive measurements to characterize the dielectric properties of cancer cells.},
   keywords = {Animals
Cell Line, Tumor
Cell Membrane/pathology
Cell Separation/methods
Electric Conductivity
Electrodes
Mice
Models, Theoretical
Neoplasm Staging/*methods
Neoplasms/*pathology
Rotation},
   ISSN = {1932-6203},
   DOI = {10.1371/journal.pone.0222289},
   year = {2019},
   type = {Journal Article}
}

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V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Trainito"],"firstnames":["C.","I."],"suffixes":[]},{"propositions":[],"lastnames":["Sweeney"],"firstnames":["D.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Čemažar"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Schmelz"],"firstnames":["E.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Français"],"firstnames":["O."],"suffixes":[]},{"propositions":[],"lastnames":["Le","Pioufle"],"firstnames":["B."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Characterization of sequentially-staged cancer cells using electrorotation","journal":"PLoS One","volume":"14","number":"9","pages":"e0222289","note":"1932-6203 Trainito, Claudia I Orcid: 0000-0002-2728-9364 Sweeney, Daniel C Orcid: 0000-0002-1289-1627 Čemažar, Jaka Schmelz, Eva M Français, Olivier Le Pioufle, Bruno Davalos, Rafael V R01 CA213423/CA/NCI NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United States 2019/09/20 PLoS One. 2019 Sep 19;14(9):e0222289. doi: 10.1371/journal.pone.0222289. eCollection 2019.","abstract":"The identification and separation of cells from heterogeneous populations is critical to the diagnosis of diseases. Label-free methodologies in particular have been developed to manipulate individual cells using properties such as density and morphology. The electrical properties of malignant cells, including the membrane capacitance and cytoplasmic conductivity, have been demonstrated to be altered compared to non-malignant cells of similar origin. Here, we exploit these changes to characterize individual cells in a sequentially-staged in vitro cancer model using electrorotation (EROT)-the rotation of a cell induced by a rotating electric field. Using a microfabricated device, a dielectrophoretic force to suspend cells while measuring their angular velocity resulting from an EROT force applied at frequencies between 3 kHz to 10 MHz. We experimentally determine the EROT response for cells at three stages of malignancy and analyze the resultant spectra by considering models that include the effect of the cell membrane alone (single-shell model) and the combined effect of the cell membrane and nucleus (double-shell model). We find that the cell membrane is largely responsible for a given cell's EROT response between 3 kHz and 10 MHz. Our results also indicate that membrane capacitance, membrane conductance, and cytoplasmic conductivity increase with an increasingly malignant phenotype. Our results demonstrate the potential of using electrorotation as a means making of non-invasive measurements to characterize the dielectric properties of cancer cells.","keywords":"Animals Cell Line, Tumor Cell Membrane/pathology Cell Separation/methods Electric Conductivity Electrodes Mice Models, Theoretical Neoplasm Staging/*methods Neoplasms/*pathology Rotation","issn":"1932-6203","doi":"10.1371/journal.pone.0222289","year":"2019","bibtex":"@article{RN144,\n author = {Trainito, C. I. and Sweeney, D. C. and Čemažar, J. and Schmelz, E. M. and Français, O. and Le Pioufle, B. and Davalos, R. V.},\n title = {Characterization of sequentially-staged cancer cells using electrorotation},\n journal = {PLoS One},\n volume = {14},\n number = {9},\n pages = {e0222289},\n note = {1932-6203\nTrainito, Claudia I\nOrcid: 0000-0002-2728-9364\nSweeney, Daniel C\nOrcid: 0000-0002-1289-1627\nČemažar, Jaka\nSchmelz, Eva M\nFrançais, Olivier\nLe Pioufle, Bruno\nDavalos, Rafael V\nR01 CA213423/CA/NCI NIH HHS/United States\nJournal Article\nResearch Support, N.I.H., Extramural\nResearch Support, Non-U.S. Gov't\nResearch Support, U.S. Gov't, Non-P.H.S.\nUnited States\n2019/09/20\nPLoS One. 2019 Sep 19;14(9):e0222289. doi: 10.1371/journal.pone.0222289. eCollection 2019.},\n abstract = {The identification and separation of cells from heterogeneous populations is critical to the diagnosis of diseases. Label-free methodologies in particular have been developed to manipulate individual cells using properties such as density and morphology. The electrical properties of malignant cells, including the membrane capacitance and cytoplasmic conductivity, have been demonstrated to be altered compared to non-malignant cells of similar origin. Here, we exploit these changes to characterize individual cells in a sequentially-staged in vitro cancer model using electrorotation (EROT)-the rotation of a cell induced by a rotating electric field. Using a microfabricated device, a dielectrophoretic force to suspend cells while measuring their angular velocity resulting from an EROT force applied at frequencies between 3 kHz to 10 MHz. We experimentally determine the EROT response for cells at three stages of malignancy and analyze the resultant spectra by considering models that include the effect of the cell membrane alone (single-shell model) and the combined effect of the cell membrane and nucleus (double-shell model). We find that the cell membrane is largely responsible for a given cell's EROT response between 3 kHz and 10 MHz. Our results also indicate that membrane capacitance, membrane conductance, and cytoplasmic conductivity increase with an increasingly malignant phenotype. Our results demonstrate the potential of using electrorotation as a means making of non-invasive measurements to characterize the dielectric properties of cancer cells.},\n keywords = {Animals\nCell Line, Tumor\nCell Membrane/pathology\nCell Separation/methods\nElectric Conductivity\nElectrodes\nMice\nModels, Theoretical\nNeoplasm Staging/*methods\nNeoplasms/*pathology\nRotation},\n ISSN = {1932-6203},\n DOI = {10.1371/journal.pone.0222289},\n year = {2019},\n type = {Journal Article}\n}\n\n","author_short":["Trainito, C. I.","Sweeney, D. C.","Čemažar, J.","Schmelz, E. M.","Français, O.","Le Pioufle, B.","Davalos, R. V."],"key":"RN144","id":"RN144","bibbaseid":"trainito-sweeney-emaar-schmelz-franais-lepioufle-davalos-characterizationofsequentiallystagedcancercellsusingelectrorotation-2019","role":"author","urls":{},"keyword":["Animals Cell Line","Tumor Cell Membrane/pathology Cell Separation/methods Electric Conductivity Electrodes Mice Models","Theoretical Neoplasm Staging/*methods Neoplasms/*pathology Rotation"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["animals cell line","tumor cell membrane/pathology cell separation/methods electric conductivity electrodes mice models","theoretical neoplasm staging/*methods neoplasms/*pathology rotation"],"search_terms":["characterization","sequentially","staged","cancer","cells","using","electrorotation","trainito","sweeney","čemažar","schmelz","français","le pioufle","davalos"],"title":"Characterization of sequentially-staged cancer cells using electrorotation","year":2019}