@Article{Trautmann2019,
  author       = {Simone Trautmann and Ahmad Barghash and Claudia Fecher-Trost and Pascal Schalkowsky and Christian Hannig and Jasmin Kirsch and Stefan Rupf and Andreas Keller and Volkhard Helms and Matthias Hannig},
  title        = {Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals},
  journal      = {PROTEOMICS–Clinical Applications},
  year         = {2019},
  volume       = {13},
  issue        = {4},
  pages        = {1800143},
  issn         = {1800143},
  issn-linking = {1800143},
  month        = jul,
  abstract     = {Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.},
  doi          = {10.1002/prca.201800143},
  pii          = {10.1002/prca.201800143},
}