Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals. Trautmann, S., Barghash, A., Fecher-Trost, C., Schalkowsky, P., Hannig, C., Kirsch, J., Rupf, S., Keller, A., Helms, V., & Hannig, M. PROTEOMICS–Clinical Applications, 13:1800143, July, 2019. doi abstract bibtex Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.
@Article{Trautmann2019,
author = {Simone Trautmann and Ahmad Barghash and Claudia Fecher-Trost and Pascal Schalkowsky and Christian Hannig and Jasmin Kirsch and Stefan Rupf and Andreas Keller and Volkhard Helms and Matthias Hannig},
title = {Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals},
journal = {PROTEOMICS–Clinical Applications},
year = {2019},
volume = {13},
issue = {4},
pages = {1800143},
issn = {1800143},
issn-linking = {1800143},
month = jul,
abstract = {Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.},
doi = {10.1002/prca.201800143},
pii = {10.1002/prca.201800143},
}
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Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.","doi":"10.1002/prca.201800143","pii":"10.1002/prca.201800143","bibtex":"@Article{Trautmann2019,\n author = {Simone Trautmann and Ahmad Barghash and Claudia Fecher-Trost and Pascal Schalkowsky and Christian Hannig and Jasmin Kirsch and Stefan Rupf and Andreas Keller and Volkhard Helms and Matthias Hannig},\n title = {Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals},\n journal = {PROTEOMICS–Clinical Applications},\n year = {2019},\n volume = {13},\n issue = {4},\n pages = {1800143},\n issn = {1800143},\n issn-linking = {1800143},\n month = jul,\n abstract = {Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.},\n doi = {10.1002/prca.201800143},\n pii = {10.1002/prca.201800143},\n}\n\n","author_short":["Trautmann, S.","Barghash, A.","Fecher-Trost, C.","Schalkowsky, P.","Hannig, C.","Kirsch, J.","Rupf, S.","Keller, A.","Helms, V.","Hannig, M."],"key":"Trautmann2019","id":"Trautmann2019","bibbaseid":"trautmann-barghash-fechertrost-schalkowsky-hannig-kirsch-rupf-keller-etal-proteomicanalysisoftheinitialoralpellicleincariesactiveandcariesfreeindividuals-2019","role":"author","urls":{},"metadata":{"authorlinks":{"keller, a":"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2023/07/references.bib_.txt&folding=1"}},"html":""},"bibtype":"article","biburl":"https://www.ccb.uni-saarland.de/wp-content/uploads/2024/01/references.bib_.txt","creationDate":"2020-02-07T09:50:48.024Z","downloads":0,"keywords":[],"search_terms":["proteomic","analysis","initial","oral","pellicle","caries","active","caries","free","individuals","trautmann","barghash","fecher-trost","schalkowsky","hannig","kirsch","rupf","keller","helms","hannig"],"title":"Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals","year":2019,"dataSources":["Tk7NyW85uR28Rhd26","k7tjjxqz46TBRgack","qqBiPXk2jEroaRXH2","9DxWazzLQoAjp9mw3","MaeSQYhi8jBE6oYaK","XSoPwnytNRZeNL8Wv","ukDDkYqwLbdhYXTJA","qd2NgSKHS68Kcdt7y","uFrEYNpx3Zmayo2AS","X7BjFZrHHnyywjGc5","iQsmnqgonvyW7tRge","RjjDBMYeiCRMZWAvn","pTW7v7XACewjrTXET","BD2qbudjMvyXtTiz5","NmhXQcJvRc2QhnSZF","ipvH6pWABxuwdKDLx","Pny5E4E9kc7C8gG8g","SiGP46KPWizw6ihLJ"]}