Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals. Trautmann, S., Barghash, A., Fecher-Trost, C., Schalkowsky, P., Hannig, C., Kirsch, J., Rupf, S., Keller, A., Helms, V., & Hannig, M. PROTEOMICS–Clinical Applications, 13:1800143, July, 2019. doi abstract bibtex Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.
@Article{Trautmann2019,
author = {Simone Trautmann and Ahmad Barghash and Claudia Fecher-Trost and Pascal Schalkowsky and Christian Hannig and Jasmin Kirsch and Stefan Rupf and Andreas Keller and Volkhard Helms and Matthias Hannig},
title = {Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals},
journal = {PROTEOMICS–Clinical Applications},
year = {2019},
volume = {13},
issue = {4},
pages = {1800143},
issn = {1800143},
issn-linking = {1800143},
month = jul,
abstract = {Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.},
doi = {10.1002/prca.201800143},
pii = {10.1002/prca.201800143},
}
Downloads: 0
{"_id":"uEriqXTXDevFknpah","bibbaseid":"trautmann-barghash-fechertrost-schalkowsky-hannig-kirsch-rupf-keller-etal-proteomicanalysisoftheinitialoralpellicleincariesactiveandcariesfreeindividuals-2019","authorIDs":["34Basrr9c8essSxF5","3H3ZGeipXPFqerDaZ","3L8KMMdHpNJwL5X59","3QW2sqLkfFtm5jHnA","3TvJ7vM9DSXRk2Bf2","3f4Gh22hZvzqSXYkq","3xrBG62gnTfT3YyHw","3yMYZYaJvy2SpaecT","3z3qjtceDFM2uuDyb","5e3d32f7c405ecde010000ee","5e3edea786a596de01000114","5e407babb531d7de010000d1","5e42261e70cecede0100012a","5e4297ba5ea111df01000153","5e42a7c2e7bb7ede0100008d","5e451d17501a51de0100011f","5e45bf920920e8de01000104","5e496da916841dde01000118","5e49b972cb98e8de010000d5","5e4bf05a8f0677df01000155","5e4bff8a0dff2bde010000b9","5e4c02920dff2bde010000ed","5e525eac2eb8f4de0100004c","5e53c83f80e18ade01000040","5e53f719d26e87df010001ab","5e544bfb7a758fde0100018c","5e5704747840dfde01000319","5e5745bebf9f82de01000105","5e579393cef9b7de01000113","5e5c2b6315d8f5de010000b0","5e5d4af273eb2edf010001f7","5e5e805e14f49fde01000174","5e5ea5782fd1fade0100005d","5e622b92a84f4adf01000053","5e64b3f2a8ac14df01000055","5e663bd3a77c4ade0100005e","5e67a442d527f0de010002ad","5e68ac2078b561de01000100","5e6960c5af718af20100073e","5e6a149f38f351de01000092","5eCpeLiD7J6ZmDE7R","6cDanA2o5HB3xEDkt","6icje6bi58uisjyaD","7BypjAfgR3uzEMhXC","87nAydG2puAvfXdoL","8CM6LdPdpSuxEgJGM","9AmuFEaDrpKqdvwdA","9QRj7dqEEDaHtxpHm","9pBpTwRtgzyTTX68p","A72ZXcNQH5g33KGKg","B3EaRfgdAaNFnz9pM","BgRuH2NfqEFDxN7ZR","BwuoXmufQpQRwAb4y","CHzPyegFZ8ecDPNFg","CJxWfidaazJ8zZ95b","CbTeq3RWwnZQYhfrT","E4pcfR8piZeRvz5PS","EBFNNGuSXgmRorbuv","ERCPXitxx3znX27Zn","F8xhM8qx7WzT9rCxt","FGsnb5iPCgEryWmsE","FtuakP48ydoaPHFjh","HXZdMjBPBBwNyfJsg","HeecQ4xM8oFp9SPmj","Kn4t55d75aGsyPXfd","LLeowHLxBLnjK7rLi","LkxHQdEZDSFT2EYGd","LnRXJ7hjr6ZmFpTrY","N3BwAdZcvanczePNg","NTsYLqLWWzz3wBARH","PZdwdFygYfrWTJRBd","PqMydQ5Y4zWxbWWfT","Q2zozb6F8XeBzYtL4","RnkbtJZszWZnz5Htg","SLrNQv5FD3sai2fxH","Sf6mpEMubavCyeLj6","Sk7f7rcBFp9Phdat6","Teeb8Xp3qjSxWMb7T","W9bWpLNKdL9FbekaP","Whgm8v3GHJLcQ5qPd","XbDKHPuEwG5WD9gFx","Xv2wCZfNAiokK29bw","Yg3Jfwsgge5K6XT8j","Z5WsEbHj8BZBRmwvc","aK5MHbueupzdB8rZZ","aQzCiysnL2jEtuSJP","anCyN3GFxK4jfF9oN","bgY9i9L3qusg99Tyw","cLbEJwXrPDSmx4G3o","cXNoPXCiGeRyfuJSL","cXhTFkuPCXPbdttQx","cxPPCjWjGcGXNzaXP","d5bCbQMWDHP8mZcap","eFahm9XJga5g2GMqG","f7yGgaXw4iZzx5RRZ","fPozz7zyv3Zp9u5NT","fhceFCP2ssXGbr7db","fyHeAsLDjsFp4j6gQ","g23gTS4FpjBZesYcL","gW7jmdjLkGSEEuqML","hSeQG75Cg8He8jCRM","hiuJLCfT4A6heYk2p","jQ53keWYCLnEnzsw5","jcMZqeeT3eN7xMY8a","jfRonvSZGHdKMAEKY","jopy9aNXuK6oMwbJB","jpWD7JhtM6tcgMkyy","kKnwKoknLQLptNu9g","kTRLGLvp3NrZjuAed","kecw8ifpKFpTssTkd","kj2ADP4KBmFdg2c2z","kv49Yd7AZcv3xLtDx","m7EuXdYideLybfk39","mJJmbPSkTZLqoZQpY","mvjsC5unk66rxYgAH","n52iKFeefcJWMs6bC","nD8TW8NBGJtoRT4oo","nfXSeP7gsGekYHARE","njXQSvJnwkdwMpDf8","nsmo7tiRtr5R4oJtL","pL3eZHvYvdAbW3pZj","pcAM6RixupDuCu8yD","px8PgMT8B2yoccJB9","r2v6zqmMoTbKQvku5","rFHSoiT9jvEKP3fEC","rthhQLP7xkjRYJr7F","s8g4J6fubzPmwbmb6","swDMSmrfNMByvAL3E","tLoSfQ9MFFncvwQ5o","tkhbK8eCwX4bpsrDS","uqPgPxPv8wujtk7HZ","vSH2CM8y5MuFoaumK","voWSPswHcXmBP8So9","vyXak4TvuBhHMEes5","wJxGrFiGZBhqiGAK9","wa8jmEhMJMSDp2Q2Y","x8BgxPMkxjWWZ3nTv","yQCeY5xQcZJH72Rpv","yxtLYyL5bDD3yBgTY","z66uEk488jQeBBrpm","zmYzipJWoWCdttGFH"],"author_short":["Trautmann, S.","Barghash, A.","Fecher-Trost, C.","Schalkowsky, P.","Hannig, C.","Kirsch, J.","Rupf, S.","Keller, A.","Helms, V.","Hannig, M."],"bibdata":{"bibtype":"article","type":"article","author":[{"firstnames":["Simone"],"propositions":[],"lastnames":["Trautmann"],"suffixes":[]},{"firstnames":["Ahmad"],"propositions":[],"lastnames":["Barghash"],"suffixes":[]},{"firstnames":["Claudia"],"propositions":[],"lastnames":["Fecher-Trost"],"suffixes":[]},{"firstnames":["Pascal"],"propositions":[],"lastnames":["Schalkowsky"],"suffixes":[]},{"firstnames":["Christian"],"propositions":[],"lastnames":["Hannig"],"suffixes":[]},{"firstnames":["Jasmin"],"propositions":[],"lastnames":["Kirsch"],"suffixes":[]},{"firstnames":["Stefan"],"propositions":[],"lastnames":["Rupf"],"suffixes":[]},{"firstnames":["Andreas"],"propositions":[],"lastnames":["Keller"],"suffixes":[]},{"firstnames":["Volkhard"],"propositions":[],"lastnames":["Helms"],"suffixes":[]},{"firstnames":["Matthias"],"propositions":[],"lastnames":["Hannig"],"suffixes":[]}],"title":"Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals","journal":"PROTEOMICS–Clinical Applications","year":"2019","volume":"13","issue":"4","pages":"1800143","issn":"1800143","issn-linking":"1800143","month":"July","abstract":"Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.","doi":"10.1002/prca.201800143","pii":"10.1002/prca.201800143","bibtex":"@Article{Trautmann2019,\n author = {Simone Trautmann and Ahmad Barghash and Claudia Fecher-Trost and Pascal Schalkowsky and Christian Hannig and Jasmin Kirsch and Stefan Rupf and Andreas Keller and Volkhard Helms and Matthias Hannig},\n title = {Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals},\n journal = {PROTEOMICS–Clinical Applications},\n year = {2019},\n volume = {13},\n issue = {4},\n pages = {1800143},\n issn = {1800143},\n issn-linking = {1800143},\n month = jul,\n abstract = {Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather “individual fingerprint”. Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.},\n doi = {10.1002/prca.201800143},\n pii = {10.1002/prca.201800143},\n}\n\n","author_short":["Trautmann, S.","Barghash, A.","Fecher-Trost, C.","Schalkowsky, P.","Hannig, C.","Kirsch, J.","Rupf, S.","Keller, A.","Helms, V.","Hannig, M."],"key":"Trautmann2019","id":"Trautmann2019","bibbaseid":"trautmann-barghash-fechertrost-schalkowsky-hannig-kirsch-rupf-keller-etal-proteomicanalysisoftheinitialoralpellicleincariesactiveandcariesfreeindividuals-2019","role":"author","urls":{},"metadata":{"authorlinks":{"keller, a":"https://bibbase.org/show?bib=https://www.ccb.uni-saarland.de/wp-content/uploads/2024/10/references.bib_.txt&folding=1"}},"downloads":0,"html":""},"bibtype":"article","biburl":"https://www.ccb.uni-saarland.de/wp-content/uploads/2024/11/references.bib_.txt","creationDate":"2020-02-07T09:50:48.024Z","downloads":0,"keywords":[],"search_terms":["proteomic","analysis","initial","oral","pellicle","caries","active","caries","free","individuals","trautmann","barghash","fecher-trost","schalkowsky","hannig","kirsch","rupf","keller","helms","hannig"],"title":"Proteomic Analysis of the Initial Oral Pellicle in Caries-Active and Caries-Free Individuals","year":2019,"dataSources":["Tk7NyW85uR28Rhd26","k7tjjxqz46TBRgack","qqBiPXk2jEroaRXH2","9DxWazzLQoAjp9mw3","MaeSQYhi8jBE6oYaK","XSoPwnytNRZeNL8Wv","ukDDkYqwLbdhYXTJA","qd2NgSKHS68Kcdt7y","uFrEYNpx3Zmayo2AS","X7BjFZrHHnyywjGc5","iQsmnqgonvyW7tRge","RjjDBMYeiCRMZWAvn","pTW7v7XACewjrTXET","BD2qbudjMvyXtTiz5","NmhXQcJvRc2QhnSZF","ipvH6pWABxuwdKDLx","Pny5E4E9kc7C8gG8g","SiGP46KPWizw6ihLJ","ZKiRa4gncFJ5e6f9M","CZZSbiMkXJgDMN2Ei","fMYw4bZ8PtmEvvgdF","XiRWyepSYzzAnCRoW","nqMohMYmMdCvacEct"]}