@article{tronnet_extracellular_2025,
	title = {Extracellular {ATP} is an environmental cue in bacteria},
	volume = {44},
	issn = {2211-1247},
	url = {https://www.sciencedirect.com/science/article/pii/S2211124725011271},
	doi = {10.1016/j.celrep.2025.116356},
	abstract = {In animals and plants, extracellular ATP (eATP) functions as a signal and regulates the immune response. During inflammation, intestinal bacteria are exposed to elevated eATP originating from the mucosa. However, whether bacteria respond to eATP is unclear. Here, we show that non-pathogenic Escherichia coli responds to eATP by modifying its transcriptional and metabolic landscapes. A genome-scale promoter library showed that the response is dependent on time, concentration, and medium and ATP specific. Second messengers and genes related to metabolism, biofilm formation, and envelope stress were regulated downstream of eATP. Metabolomics confirmed that eATP triggers enrichment of compounds with bioactive properties in the host or bacteria. Combined genome-scale modeling revealed modifications to global metabolic and biomass building blocks. Consequently, eATP altered the sensitivity to antibiotics and antimicrobial peptides. Finally, in pathogens, eATP controlled virulence factor expression. Our results indicate that eATP is an environmental cue in prokaryotes, which broadly regulates physiology, antimicrobial resistance, and virulence.},
	number = {10},
	urldate = {2025-10-17},
	journal = {Cell Reports},
	author = {Tronnet, Sophie and Pandey, Vikash and Lloret-Berrocal, Miriam and Pérez-del-Pozo, Mario and Hernández-Ortego, Carlos and Söderholm, Niklas and Billker, Oliver and Nordström, Anders and Puhar, Andrea},
	month = oct,
	year = {2025},
	keywords = {Enterobacteriaceae, antimicrobial resistance, extracellular ATP, gene expression, inflammation, intestinal bacteria, metabolites, physiology, purinergic signaling, virulence},
	pages = {116356},
}

{"_id":"s87K8ppRmJiy5CQ68","bibbaseid":"tronnet-pandey-lloretberrocal-prezdelpozo-hernndezortego-sderholm-billker-nordstrm-etal-extracellularatpisanenvironmentalcueinbacteria-2025","author_short":["Tronnet, S.","Pandey, V.","Lloret-Berrocal, M.","Pérez-del-Pozo, M.","Hernández-Ortego, C.","Söderholm, N.","Billker, O.","Nordström, A.","Puhar, A."],"bibdata":{"bibtype":"article","type":"article","title":"Extracellular ATP is an environmental cue in bacteria","volume":"44","issn":"2211-1247","url":"https://www.sciencedirect.com/science/article/pii/S2211124725011271","doi":"10.1016/j.celrep.2025.116356","abstract":"In animals and plants, extracellular ATP (eATP) functions as a signal and regulates the immune response. During inflammation, intestinal bacteria are exposed to elevated eATP originating from the mucosa. However, whether bacteria respond to eATP is unclear. Here, we show that non-pathogenic Escherichia coli responds to eATP by modifying its transcriptional and metabolic landscapes. A genome-scale promoter library showed that the response is dependent on time, concentration, and medium and ATP specific. Second messengers and genes related to metabolism, biofilm formation, and envelope stress were regulated downstream of eATP. Metabolomics confirmed that eATP triggers enrichment of compounds with bioactive properties in the host or bacteria. Combined genome-scale modeling revealed modifications to global metabolic and biomass building blocks. Consequently, eATP altered the sensitivity to antibiotics and antimicrobial peptides. Finally, in pathogens, eATP controlled virulence factor expression. Our results indicate that eATP is an environmental cue in prokaryotes, which broadly regulates physiology, antimicrobial resistance, and virulence.","number":"10","urldate":"2025-10-17","journal":"Cell Reports","author":[{"propositions":[],"lastnames":["Tronnet"],"firstnames":["Sophie"],"suffixes":[]},{"propositions":[],"lastnames":["Pandey"],"firstnames":["Vikash"],"suffixes":[]},{"propositions":[],"lastnames":["Lloret-Berrocal"],"firstnames":["Miriam"],"suffixes":[]},{"propositions":[],"lastnames":["Pérez-del-Pozo"],"firstnames":["Mario"],"suffixes":[]},{"propositions":[],"lastnames":["Hernández-Ortego"],"firstnames":["Carlos"],"suffixes":[]},{"propositions":[],"lastnames":["Söderholm"],"firstnames":["Niklas"],"suffixes":[]},{"propositions":[],"lastnames":["Billker"],"firstnames":["Oliver"],"suffixes":[]},{"propositions":[],"lastnames":["Nordström"],"firstnames":["Anders"],"suffixes":[]},{"propositions":[],"lastnames":["Puhar"],"firstnames":["Andrea"],"suffixes":[]}],"month":"October","year":"2025","keywords":"Enterobacteriaceae, antimicrobial resistance, extracellular ATP, gene expression, inflammation, intestinal bacteria, metabolites, physiology, purinergic signaling, virulence","pages":"116356","bibtex":"@article{tronnet_extracellular_2025,\n\ttitle = {Extracellular {ATP} is an environmental cue in bacteria},\n\tvolume = {44},\n\tissn = {2211-1247},\n\turl = {https://www.sciencedirect.com/science/article/pii/S2211124725011271},\n\tdoi = {10.1016/j.celrep.2025.116356},\n\tabstract = {In animals and plants, extracellular ATP (eATP) functions as a signal and regulates the immune response. During inflammation, intestinal bacteria are exposed to elevated eATP originating from the mucosa. However, whether bacteria respond to eATP is unclear. Here, we show that non-pathogenic Escherichia coli responds to eATP by modifying its transcriptional and metabolic landscapes. A genome-scale promoter library showed that the response is dependent on time, concentration, and medium and ATP specific. Second messengers and genes related to metabolism, biofilm formation, and envelope stress were regulated downstream of eATP. Metabolomics confirmed that eATP triggers enrichment of compounds with bioactive properties in the host or bacteria. Combined genome-scale modeling revealed modifications to global metabolic and biomass building blocks. Consequently, eATP altered the sensitivity to antibiotics and antimicrobial peptides. Finally, in pathogens, eATP controlled virulence factor expression. Our results indicate that eATP is an environmental cue in prokaryotes, which broadly regulates physiology, antimicrobial resistance, and virulence.},\n\tnumber = {10},\n\turldate = {2025-10-17},\n\tjournal = {Cell Reports},\n\tauthor = {Tronnet, Sophie and Pandey, Vikash and Lloret-Berrocal, Miriam and Pérez-del-Pozo, Mario and Hernández-Ortego, Carlos and Söderholm, Niklas and Billker, Oliver and Nordström, Anders and Puhar, Andrea},\n\tmonth = oct,\n\tyear = {2025},\n\tkeywords = {Enterobacteriaceae, antimicrobial resistance, extracellular ATP, gene expression, inflammation, intestinal bacteria, metabolites, physiology, purinergic signaling, virulence},\n\tpages = {116356},\n}\n\n\n\n\n\n\n\n","author_short":["Tronnet, S.","Pandey, V.","Lloret-Berrocal, M.","Pérez-del-Pozo, M.","Hernández-Ortego, C.","Söderholm, N.","Billker, O.","Nordström, A.","Puhar, A."],"key":"tronnet_extracellular_2025","id":"tronnet_extracellular_2025","bibbaseid":"tronnet-pandey-lloretberrocal-prezdelpozo-hernndezortego-sderholm-billker-nordstrm-etal-extracellularatpisanenvironmentalcueinbacteria-2025","role":"author","urls":{"Paper":"https://www.sciencedirect.com/science/article/pii/S2211124725011271"},"keyword":["Enterobacteriaceae","antimicrobial resistance","extracellular ATP","gene expression","inflammation","intestinal bacteria","metabolites","physiology","purinergic signaling","virulence"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs"],"keywords":["enterobacteriaceae","antimicrobial resistance","extracellular atp","gene expression","inflammation","intestinal bacteria","metabolites","physiology","purinergic signaling","virulence"],"search_terms":["extracellular","atp","environmental","cue","bacteria","tronnet","pandey","lloret-berrocal","pérez-del-pozo","hernández-ortego","söderholm","billker","nordström","puhar"],"title":"Extracellular ATP is an environmental cue in bacteria","year":2025}