Deep and fast live imaging with two-photon scanned light-sheet microscopy. Truong, T. V., Supatto, W., Koos, D. S., Choi, J. M., & Fraser, S. E. Nature Methods, 8(9):757--760, September, 2011. Paper doi abstract bibtex We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
@article{truong_deep_2011,
title = {Deep and fast live imaging with two-photon scanned light-sheet microscopy},
volume = {8},
copyright = {© 2011 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.},
issn = {1548-7091},
url = {http://www.nature.com/nmeth/journal/v8/n9/abs/nmeth.1652.html},
doi = {10.1038/nmeth.1652},
abstract = {We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.},
number = {9},
urldate = {2016-03-20TZ},
journal = {Nature Methods},
author = {Truong, Thai V. and Supatto, Willy and Koos, David S. and Choi, John M. and Fraser, Scott E.},
month = sep,
year = {2011},
pages = {757--760}
}
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