Deep and fast live imaging with two-photon scanned light-sheet microscopy. Truong, T. V., Supatto, W., Koos, D. S., Choi, J. M., & Fraser, S. E. Nature Methods, 8(9):757--760, September, 2011.
Deep and fast live imaging with two-photon scanned light-sheet microscopy [link]Paper  doi  abstract   bibtex   
We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
@article{truong_deep_2011,
	title = {Deep and fast live imaging with two-photon scanned light-sheet microscopy},
	volume = {8},
	copyright = {© 2011 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.},
	issn = {1548-7091},
	url = {http://www.nature.com/nmeth/journal/v8/n9/abs/nmeth.1652.html},
	doi = {10.1038/nmeth.1652},
	abstract = {We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.},
	number = {9},
	urldate = {2016-03-20TZ},
	journal = {Nature Methods},
	author = {Truong, Thai V. and Supatto, Willy and Koos, David S. and Choi, John M. and Fraser, Scott E.},
	month = sep,
	year = {2011},
	pages = {757--760}
}

Downloads: 0