New PEO-IAA-Inspired Anti-Auxins: Synthesis, Biological Activity, and Possible Application in Hemp (Cannabis Sativa L.) Micropropagation

New PEO-IAA-Inspired Anti-Auxins: Synthesis, Biological Activity, and Possible Application in Hemp (Cannabis Sativa L.) Micropropagation. Žukauskaitė, A., Saiz-Fernández, I., Bieleszová, K., Iškauskienė, M., Zhang, C., Smýkalová, I., Dzedulionytė, K., Kubeš, M. F., Sedlářová, M., Pařízková, B., Pavlović, I., Vain, T., Petřík, I., Malinauskienė, V., Šačkus, A., Strnad, M., Robert, S., Napier, R., Novák, O., & Doležal, K. Journal of Plant Growth Regulation, May, 2023.

Paper doi abstract bibtex

Auxins play an important role in plant physiology and are involved in numerous aspects of plant development, such as cell division, elongation and differentiation, fruit development, and phototropic response. In addition, through their antagonistic interaction with cytokinins, auxins play a key role in the regulation of root growth and apical dominance. Thanks to this capacity to determine plant architecture, natural and synthetic auxins have been successfully employed to obtain more economically advantageous plants. The crosstalk between auxins and cytokinins determines plant development and thus is of particular importance in the field of plant micropropagation, where the ratios between these two phytohormones need to be tightly controlled to achieve proper rooting and shoot generation. Previously reported anti-auxin PEO-IAA, which blocks auxin signalling through binding to TIR1 receptor and inhibiting the expression of auxin-responsive genes, has been successfully used to facilitate hemp micropropagation. Herein, we report a set of new PEO-IAA-inspired anti-auxins capable of antagonizing auxin responses in vivo. The capacity of these compounds to bind to the TIR1 receptor was confirmed in vitro by SPR analysis. Using DESI-MSI analysis, we evaluated the uptake and distribution of the compounds at the whole plant level. Finally, we characterized the effect of the compounds on the organogenesis of hemp explants, where they showed to be able to improve beneficial morphological traits, such as the balanced growth of all the produced shoots and enhanced bud proliferation.

@article{zukauskaite_new_2023,
	title = {New {PEO}-{IAA}-{Inspired} {Anti}-{Auxins}: {Synthesis}, {Biological} {Activity}, and {Possible} {Application} in {Hemp} ({Cannabis} {Sativa} {L}.) {Micropropagation}},
	issn = {1435-8107},
	shorttitle = {New {PEO}-{IAA}-{Inspired} {Anti}-{Auxins}},
	url = {https://doi.org/10.1007/s00344-023-11031-x},
	doi = {10.1007/s00344-023-11031-x},
	abstract = {Auxins play an important role in plant physiology and are involved in numerous aspects of plant development, such as cell division, elongation and differentiation, fruit development, and phototropic response. In addition, through their antagonistic interaction with cytokinins, auxins play a key role in the regulation of root growth and apical dominance. Thanks to this capacity to determine plant architecture, natural and synthetic auxins have been successfully employed to obtain more economically advantageous plants. The crosstalk between auxins and cytokinins determines plant development and thus is of particular importance in the field of plant micropropagation, where the ratios between these two phytohormones need to be tightly controlled to achieve proper rooting and shoot generation. Previously reported anti-auxin PEO-IAA, which blocks auxin signalling through binding to TIR1 receptor and inhibiting the expression of auxin-responsive genes, has been successfully used to facilitate hemp micropropagation. Herein, we report a set of new PEO-IAA-inspired anti-auxins capable of antagonizing auxin responses in vivo. The capacity of these compounds to bind to the TIR1 receptor was confirmed in vitro by SPR analysis. Using DESI-MSI analysis, we evaluated the uptake and distribution of the compounds at the whole plant level. Finally, we characterized the effect of the compounds on the organogenesis of hemp explants, where they showed to be able to improve beneficial morphological traits, such as the balanced growth of all the produced shoots and enhanced bud proliferation.},
	language = {en},
	urldate = {2023-06-09},
	journal = {Journal of Plant Growth Regulation},
	author = {Žukauskaitė, Asta and Saiz-Fernández, Iñigo and Bieleszová, Kristýna and Iškauskienė, Monika and Zhang, Chao and Smýkalová, Iva and Dzedulionytė, Karolina and Kubeš, Martin F. and Sedlářová, Michaela and Pařízková, Barbora and Pavlović, Iva and Vain, Thomas and Petřík, Ivan and Malinauskienė, Vida and Šačkus, Algirdas and Strnad, Miroslav and Robert, Stéphanie and Napier, Richard and Novák, Ondřej and Doležal, Karel},
	month = may,
	year = {2023},
	keywords = {Anti-auxin, Arabidopsis thaliana, DESI-MSI analysis, Indole-3-acetic acid (IAA), Multiple shoot culture, SPR analysis},
}

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