Photobiological characteristics of chlorophyll a derivatives as microbial PDT agents. Uliana, M., P., Pires, L., Pratavieira, S., Brocksom, T., J., de Oliveira, K., T., Bagnato, V., S., & Kurachi, C. Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 13:1137-45, 2014.
Photobiological characteristics of chlorophyll a derivatives as microbial PDT agents. [pdf]Paper  Photobiological characteristics of chlorophyll a derivatives as microbial PDT agents. [link]Website  abstract   bibtex   
Chlorin-e6 () and a hydrogenated derivative () were semi-synthesized, and their photophysical properties and photodynamic activity against Escherichia coli, Staphylococcus aureus and Candida albicans evaluated. Methyl pheophorbide-a () was obtained from S. maxima using methanolic extraction with acid catalysis (CH3OH-H2SO4). Chlorin-e6 was prepared from by basic hydrolysis with H2O-acetone and NaOH. Hydrogenated Chlorin-e6 was synthesized by a similar procedure starting from the hydrogenated methyl pheophorbide-a (). Photophysical studies were performed in order to determine the singlet oxygen quantum yield of which is higher than that of . The microorganism inactivation of and was investigated at two concentrations and three fluence levels. Both and showed microorganism inactivation against Gram-positive bacteria and a fungus.

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