Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis. Ushimaru, Y., Konno, A., Kaizu, M., Ogawa, K., Satoh, N., & Inaba, K. Zoological Science, 23(8):679–687, August, 2006. Publisher: Zoological Society of Japan
Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis [link]Paper  doi  abstract   bibtex   
We previously identified a 66 kDa axonemal protein (Ci-Axp66.0) in sperm of the ascidian Ciona intestinalis, Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chlamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66.0 suggested that the N-terminal two-thirds of the molecule is rich in coiled coil structure, as in Chlamydomonas DC2. Immunogold localization revealed that it is located in the vicinity of outer arm dynein. Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. This co-purification was not affected by the absence of Mg2 in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. These results suggest that Ci-Axp66.0 is a component of the outer arm dynein docking complex in the axonemes of Ciona sperm.
@article{ushimaru_association_2006,
	title = {Association of a 66 {kDa} {Homolog} of {Chlamydomonas} {DC2}, a {Subunit} of the {Outer} {Arm} {Docking} {Complex}, with {Outer} {Arm} {Dynein} of {Sperm} {Flagella} in the {Ascidian} {Ciona} intestinalis},
	volume = {23},
	issn = {0289-0003},
	url = {https://bioone.org/journals/Zoological-Science/volume-23/issue-8/zsj.23.679/Association-of-a-66-kDa-Homolog-of-Chlamydomonas-DC2-a/10.2108/zsj.23.679.full},
	doi = {10.2108/zsj.23.679},
	abstract = {We previously identified a 66 kDa axonemal protein (Ci-Axp66.0) in sperm of the ascidian Ciona intestinalis, Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chlamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66.0 suggested that the N-terminal two-thirds of the molecule is rich in coiled coil structure, as in Chlamydomonas DC2. Immunogold localization revealed that it is located in the vicinity of outer arm dynein. Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. This co-purification was not affected by the absence of Mg2 in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. These results suggest that Ci-Axp66.0 is a component of the outer arm dynein docking complex in the axonemes of Ciona sperm.},
	number = {8},
	urldate = {2020-03-03},
	journal = {Zoological Science},
	author = {Ushimaru, Yuji and Konno, Aru and Kaizu, Maiko and Ogawa, Kazuo and Satoh, Nori and Inaba, Kazuo},
	month = aug,
	year = {2006},
	note = {Publisher: Zoological Society of Japan},
	keywords = {Inaba K},
	pages = {679--687}
}

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