@article{
 title = {Effect of indirect nonequilibrium atmospheric pressure plasma on anti-proliferative activity against chronic chemo-resistant ovarian cancer cells <i>in vitro</i> and <i>in vivo</i>},
 type = {article},
 year = {2013},
 identifiers = {[object Object]},
 pages = {e81576},
 volume = {8},
 id = {7ade74fc-264f-3cb5-8f51-0c6db797597a},
 created = {2019-10-15T17:13:27.792Z},
 file_attached = {false},
 profile_id = {2a2c3e5b-5c95-3724-8df1-a9f724184d4e},
 group_id = {ade40bc4-6c2b-34a6-b26a-aad2abaa9249},
 last_modified = {2019-10-15T17:13:27.792Z},
 read = {true},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 private_publication = {false},
 abstract = {PURPOSE: Nonequilibrium atmospheric pressure plasma (NEAPP) therapy has recently been focused on as a novel medical practice. Using cells with acquired paclitaxel/cisplatin resistance, we elucidated effects of indirect NEAPP-activated medium (NEAPP-AM) exposure on cell viability and tumor growth in vitro and in vivo.\n\nMETHODS: Using chronic paclitaxel/cisplatin-resistant ovarian cancer cells, we applied indirect NEAPP-exposed medium to cells and xenografted tumors in a mouse model. Furthermore, we examined the role of reactive oxygen species (ROS) or their scavengers in the above-mentioned EOC cells.\n\nRESULTS: We assessed the viability of NOS2 and NOS3 cells exposed to NEAPP-AM, which was prepared beforehand by irradiation with NEAPP for the indicated time. In NOS2 cells, viability decreased by approximately 30% after NEAPP-AM 120-sec treatment (P<0.01). The growth-inhibitory effects of NEAPP-AM were completely inhibited by N-acetyl cysteine treatment, while L-buthionine-[S, R]-sulfoximine, an inhibitor of the ROS scavenger used with NEAPP-AM, decreased cell viability by 85% after NEAPP-AM 60-sec treatment(P<0.05) and by 52% after 120 sec, compared to the control (P<0.01). In the murine subcutaneous tumor-formation model, NEAPP-AM injection resulted in an average inhibition of the NOS2 cell-inoculated tumor by 66% (P<0.05) and NOS2TR cell-inoculated tumor by 52% (P<0.05), as compared with the control.\n\nCONCLUSION: We demonstrated that plasma-activated medium also had an anti-tumor effect on chemo-resistant cells in vitro and in vivo. Indirect plasma therapy is a promising treatment option for EOC and may contribute to a better patient prognosis in the future.},
 bibtype = {article},
 author = {Utsumi, Fumi and Kajiyama, Hiroaki and Nakamura, Kae and Tanaka, Hiromasa and Mizuno, Masaaki and Ishikawa, Kenji and Kondo, Hiroki and Kano, Hiroyuki and Hori, Masaru and Kikkawa, Fumitaka},
 journal = {PLoS ONE},
 number = {12}
}

{"_id":"6ZQepqexMLKf7eytd","bibbaseid":"utsumi-kajiyama-nakamura-tanaka-mizuno-ishikawa-kondo-kano-etal-effectofindirectnonequilibriumatmosphericpressureplasmaonantiproliferativeactivityagainstchronicchemoresistantovariancancercellsiinvitroiandiinvivoi-2013","authorIDs":[],"author_short":["Utsumi, F.","Kajiyama, H.","Nakamura, K.","Tanaka, H.","Mizuno, M.","Ishikawa, K.","Kondo, H.","Kano, H.","Hori, M.","Kikkawa, F."],"bibdata":{"title":"Effect of indirect nonequilibrium atmospheric pressure plasma on anti-proliferative activity against chronic chemo-resistant ovarian cancer cells <i>in vitro</i> and <i>in vivo</i>","type":"article","year":"2013","identifiers":"[object Object]","pages":"e81576","volume":"8","id":"7ade74fc-264f-3cb5-8f51-0c6db797597a","created":"2019-10-15T17:13:27.792Z","file_attached":false,"profile_id":"2a2c3e5b-5c95-3724-8df1-a9f724184d4e","group_id":"ade40bc4-6c2b-34a6-b26a-aad2abaa9249","last_modified":"2019-10-15T17:13:27.792Z","read":"true","starred":false,"authored":false,"confirmed":"true","hidden":false,"private_publication":false,"abstract":"PURPOSE: Nonequilibrium atmospheric pressure plasma (NEAPP) therapy has recently been focused on as a novel medical practice. Using cells with acquired paclitaxel/cisplatin resistance, we elucidated effects of indirect NEAPP-activated medium (NEAPP-AM) exposure on cell viability and tumor growth in vitro and in vivo.\\n\\nMETHODS: Using chronic paclitaxel/cisplatin-resistant ovarian cancer cells, we applied indirect NEAPP-exposed medium to cells and xenografted tumors in a mouse model. Furthermore, we examined the role of reactive oxygen species (ROS) or their scavengers in the above-mentioned EOC cells.\\n\\nRESULTS: We assessed the viability of NOS2 and NOS3 cells exposed to NEAPP-AM, which was prepared beforehand by irradiation with NEAPP for the indicated time. In NOS2 cells, viability decreased by approximately 30% after NEAPP-AM 120-sec treatment (P<0.01). The growth-inhibitory effects of NEAPP-AM were completely inhibited by N-acetyl cysteine treatment, while L-buthionine-[S, R]-sulfoximine, an inhibitor of the ROS scavenger used with NEAPP-AM, decreased cell viability by 85% after NEAPP-AM 60-sec treatment(P<0.05) and by 52% after 120 sec, compared to the control (P<0.01). In the murine subcutaneous tumor-formation model, NEAPP-AM injection resulted in an average inhibition of the NOS2 cell-inoculated tumor by 66% (P<0.05) and NOS2TR cell-inoculated tumor by 52% (P<0.05), as compared with the control.\\n\\nCONCLUSION: We demonstrated that plasma-activated medium also had an anti-tumor effect on chemo-resistant cells in vitro and in vivo. Indirect plasma therapy is a promising treatment option for EOC and may contribute to a better patient prognosis in the future.","bibtype":"article","author":"Utsumi, Fumi and Kajiyama, Hiroaki and Nakamura, Kae and Tanaka, Hiromasa and Mizuno, Masaaki and Ishikawa, Kenji and Kondo, Hiroki and Kano, Hiroyuki and Hori, Masaru and Kikkawa, Fumitaka","journal":"PLoS ONE","number":"12","bibtex":"@article{\n title = {Effect of indirect nonequilibrium atmospheric pressure plasma on anti-proliferative activity against chronic chemo-resistant ovarian cancer cells <i>in vitro</i> and <i>in vivo</i>},\n type = {article},\n year = {2013},\n identifiers = {[object Object]},\n pages = {e81576},\n volume = {8},\n id = {7ade74fc-264f-3cb5-8f51-0c6db797597a},\n created = {2019-10-15T17:13:27.792Z},\n file_attached = {false},\n profile_id = {2a2c3e5b-5c95-3724-8df1-a9f724184d4e},\n group_id = {ade40bc4-6c2b-34a6-b26a-aad2abaa9249},\n last_modified = {2019-10-15T17:13:27.792Z},\n read = {true},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {PURPOSE: Nonequilibrium atmospheric pressure plasma (NEAPP) therapy has recently been focused on as a novel medical practice. Using cells with acquired paclitaxel/cisplatin resistance, we elucidated effects of indirect NEAPP-activated medium (NEAPP-AM) exposure on cell viability and tumor growth in vitro and in vivo.\\n\\nMETHODS: Using chronic paclitaxel/cisplatin-resistant ovarian cancer cells, we applied indirect NEAPP-exposed medium to cells and xenografted tumors in a mouse model. Furthermore, we examined the role of reactive oxygen species (ROS) or their scavengers in the above-mentioned EOC cells.\\n\\nRESULTS: We assessed the viability of NOS2 and NOS3 cells exposed to NEAPP-AM, which was prepared beforehand by irradiation with NEAPP for the indicated time. In NOS2 cells, viability decreased by approximately 30% after NEAPP-AM 120-sec treatment (P<0.01). The growth-inhibitory effects of NEAPP-AM were completely inhibited by N-acetyl cysteine treatment, while L-buthionine-[S, R]-sulfoximine, an inhibitor of the ROS scavenger used with NEAPP-AM, decreased cell viability by 85% after NEAPP-AM 60-sec treatment(P<0.05) and by 52% after 120 sec, compared to the control (P<0.01). In the murine subcutaneous tumor-formation model, NEAPP-AM injection resulted in an average inhibition of the NOS2 cell-inoculated tumor by 66% (P<0.05) and NOS2TR cell-inoculated tumor by 52% (P<0.05), as compared with the control.\\n\\nCONCLUSION: We demonstrated that plasma-activated medium also had an anti-tumor effect on chemo-resistant cells in vitro and in vivo. Indirect plasma therapy is a promising treatment option for EOC and may contribute to a better patient prognosis in the future.},\n bibtype = {article},\n author = {Utsumi, Fumi and Kajiyama, Hiroaki and Nakamura, Kae and Tanaka, Hiromasa and Mizuno, Masaaki and Ishikawa, Kenji and Kondo, Hiroki and Kano, Hiroyuki and Hori, Masaru and Kikkawa, Fumitaka},\n journal = {PLoS ONE},\n number = {12}\n}","author_short":["Utsumi, F.","Kajiyama, H.","Nakamura, K.","Tanaka, H.","Mizuno, M.","Ishikawa, K.","Kondo, H.","Kano, H.","Hori, M.","Kikkawa, F."],"bibbaseid":"utsumi-kajiyama-nakamura-tanaka-mizuno-ishikawa-kondo-kano-etal-effectofindirectnonequilibriumatmosphericpressureplasmaonantiproliferativeactivityagainstchronicchemoresistantovariancancercellsiinvitroiandiinvivoi-2013","role":"author","urls":{},"downloads":0},"bibtype":"article","creationDate":"2020-03-16T16:02:19.468Z","downloads":0,"keywords":[],"search_terms":["effect","indirect","nonequilibrium","atmospheric","pressure","plasma","anti","proliferative","activity","against","chronic","chemo","resistant","ovarian","cancer","cells","vitro","vivo","utsumi","kajiyama","nakamura","tanaka","mizuno","ishikawa","kondo","kano","hori","kikkawa"],"title":"Effect of indirect nonequilibrium atmospheric pressure plasma on anti-proliferative activity against chronic chemo-resistant ovarian cancer cells <i>in vitro</i> and <i>in vivo</i>","year":2013}