Prevalence, genetic diversity and recombination of species G enteroviruses infecting pigs in Vietnam. Van Dung, N., Anh, P. H., Van Cuong, N., Hoa, N. T., Carrique-Mas, J., Hien, V. B., Campbell, J., Baker, S., Farrar, J., Woolhouse, M. E., Bryant, J. E., & Simmonds, P. The Journal of general virology, 95(Pt 3):549–556, March, 2014.
doi  abstract   bibtex   
Picornaviruses infecting pigs, described for many years as 'porcine enteroviruses', have recently been recognized as distinct viruses within three distinct genera (Teschovirus, Sapelovirus and Enterovirus). To better characterize the epidemiology and genetic diversity of members of the Enterovirus genus, faecal samples from pigs from four provinces in Vietnam were screened by PCR using conserved enterovirus (EV)-specific primers from the 5' untranslated region (5' UTR). High rates of infection were recorded in pigs on all farms, with detection frequencies of approximately 90% in recently weaned pigs but declining to 40% in those aged over 1 year. No differences in EV detection rates were observed between pigs with and without diarrhoea [74% (n = 70) compared with 72% (n = 128)]. Genetic analysis of consensus VP4/VP2 and VP1 sequences amplified from a subset of EV-infected pigs identified species G EVs in all samples. Among these, VP1 sequence comparisons identified six type 1 and seven type 6 variants, while four further VP1 sequences failed to group with any previously identified
@article{van_dung_prevalence_2014,
	title = {Prevalence, genetic diversity and recombination of species {G} enteroviruses infecting pigs in {Vietnam}.},
	volume = {95},
	issn = {1465-2099 0022-1317},
	doi = {10.1099/vir.0.061978-0},
	abstract = {Picornaviruses infecting pigs, described for many years as 'porcine enteroviruses', have recently been recognized as distinct viruses within three distinct genera (Teschovirus, Sapelovirus and Enterovirus). To better characterize the epidemiology and genetic diversity of members of the Enterovirus genus, faecal samples from pigs from four provinces in Vietnam were screened by PCR using conserved enterovirus (EV)-specific primers from the 5' untranslated region (5' UTR). High rates of infection were recorded in pigs on all farms, with detection frequencies of approximately 90\% in recently weaned pigs but declining  to 40\% in those aged over 1 year. No differences in EV detection rates were observed between pigs with and without diarrhoea [74\% (n = 70) compared with 72\%  (n = 128)]. Genetic analysis of consensus VP4/VP2 and VP1 sequences amplified from a subset of EV-infected pigs identified species G EVs in all samples. Among  these, VP1 sequence comparisons identified six type 1 and seven type 6 variants,  while four further VP1 sequences failed to group with any previously identified},
	language = {eng},
	number = {Pt 3},
	journal = {The Journal of general virology},
	author = {Van Dung, Nguyen and Anh, Pham Hong and Van Cuong, Nguyen and Hoa, Ngo Thi and Carrique-Mas, Juan and Hien, Vo Be and Campbell, James and Baker, Stephen and Farrar, Jeremy and Woolhouse, Mark E. and Bryant, Juliet E. and Simmonds, Peter},
	month = mar,
	year = {2014},
	pmid = {24323635},
	pmcid = {PMC4029279},
	keywords = {*Genetic Variation, *Recombination, Genetic, Animals, Enterovirus Infections/epidemiology/*veterinary/virology, Enteroviruses, Porcine/classification/*genetics/isolation \& purification, Molecular Sequence Data, Phylogeny, Prevalence, Sus scrofa, Swine, Swine Diseases/epidemiology/*virology, Vietnam/epidemiology, Viral Proteins/genetics},
	pages = {549--556},
}

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