epiGBS: reference-free reduced representation bisulfite sequencing. van Gurp, T., P., Wagemaker, N., C., A., M., Wouters, B., Vergeer, P., Ouborg, J., N., J., & Verhoeven, K., J., F. Nature Methods, 13(4):322-4, Nature Publishing Group, 2, 2016. ![epiGBS: reference-free reduced representation bisulfite sequencing [link]](https://bibbase.org/img/filetypes/link.svg "link")
Website abstract bibtex We describe epiGBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNPs) versus methylation variation (software is available at https://github.com/thomasvangurp/epiGBS). The output can be loaded directly into a genome browser for visualization and into RnBeads for analysis of differential methylation.
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author = {van Gurp, Thomas P and Wagemaker, Niels C A M and Wouters, Björn and Vergeer, Philippine and Ouborg, Joop N J and Verhoeven, Koen J F},
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