A new assay for anti-DNA antibodies in serum which includes the measurement of anti-Z-DNA. Van Helden, P. D., Van Lill, L., Bester, A. J., De Waal, L., & Hoal-van Helden, E. G. Clinical and Experimental Immunology, 69(2):394–402, August, 1987. abstract bibtex A simple, rapid assay for measuring anti-DNA titre of serum which includes anti-Z-DNA is described. The assay involves solution binding of antibody to labelled DNA under conditions such that the DNA is altered to form a left-handed or Z-DNA structure in the presence of cobalt ions. The absence or presence of cobalt determines a B or Z form structure in DNA and antibodies to these forms are detectable. The majority of SLE and RA patients (88%) have a higher anti-DNA titre in the presence of cobalt ions. An additional 25% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. These results suggest that monitoring anti-DNA levels in SLE and RA to detect anti-Z DNA antibodies, provides significant advantages over methods currently in use to measure anti-DNA antibodies.
@article{van_helden_new_1987,
title = {A new assay for anti-{DNA} antibodies in serum which includes the measurement of anti-{Z}-{DNA}},
volume = {69},
issn = {0009-9104},
abstract = {A simple, rapid assay for measuring anti-DNA titre of serum which includes anti-Z-DNA is described. The assay involves solution binding of antibody to labelled DNA under conditions such that the DNA is altered to form a left-handed or Z-DNA structure in the presence of cobalt ions. The absence or presence of cobalt determines a B or Z form structure in DNA and antibodies to these forms are detectable. The majority of SLE and RA patients (88\%) have a higher anti-DNA titre in the presence of cobalt ions. An additional 25\% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. These results suggest that monitoring anti-DNA levels in SLE and RA to detect anti-Z DNA antibodies, provides significant advantages over methods currently in use to measure anti-DNA antibodies.},
language = {eng},
number = {2},
journal = {Clinical and Experimental Immunology},
author = {Van Helden, P. D. and Van Lill, L. and Bester, A. J. and De Waal, L. and Hoal-van Helden, E. G.},
month = aug,
year = {1987},
pmid = {3498594},
pmcid = {PMC1542401},
keywords = {Antibodies, Antinuclear, Arthritis, Rheumatoid, Cobalt, DNA, DNA, Single-Stranded, Humans, Lupus Erythematosus, Systemic, Polydeoxyribonucleotides, Radioimmunoassay},
pages = {394--402},
}
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An additional 25% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. 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The assay involves solution binding of antibody to labelled DNA under conditions such that the DNA is altered to form a left-handed or Z-DNA structure in the presence of cobalt ions. The absence or presence of cobalt determines a B or Z form structure in DNA and antibodies to these forms are detectable. The majority of SLE and RA patients (88\\%) have a higher anti-DNA titre in the presence of cobalt ions. An additional 25\\% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. 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