A new assay for anti-DNA antibodies in serum which includes the measurement of anti-Z-DNA. Van Helden, P. D., Van Lill, L., Bester, A. J., De Waal, L., & Hoal-van Helden, E. G. Clinical and Experimental Immunology, 69(2):394–402, August, 1987. abstract bibtex A simple, rapid assay for measuring anti-DNA titre of serum which includes anti-Z-DNA is described. The assay involves solution binding of antibody to labelled DNA under conditions such that the DNA is altered to form a left-handed or Z-DNA structure in the presence of cobalt ions. The absence or presence of cobalt determines a B or Z form structure in DNA and antibodies to these forms are detectable. The majority of SLE and RA patients (88%) have a higher anti-DNA titre in the presence of cobalt ions. An additional 25% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. These results suggest that monitoring anti-DNA levels in SLE and RA to detect anti-Z DNA antibodies, provides significant advantages over methods currently in use to measure anti-DNA antibodies.
@article{van_helden_new_1987,
title = {A new assay for anti-{DNA} antibodies in serum which includes the measurement of anti-{Z}-{DNA}},
volume = {69},
issn = {0009-9104},
abstract = {A simple, rapid assay for measuring anti-DNA titre of serum which includes anti-Z-DNA is described. The assay involves solution binding of antibody to labelled DNA under conditions such that the DNA is altered to form a left-handed or Z-DNA structure in the presence of cobalt ions. The absence or presence of cobalt determines a B or Z form structure in DNA and antibodies to these forms are detectable. The majority of SLE and RA patients (88\%) have a higher anti-DNA titre in the presence of cobalt ions. An additional 25\% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. These results suggest that monitoring anti-DNA levels in SLE and RA to detect anti-Z DNA antibodies, provides significant advantages over methods currently in use to measure anti-DNA antibodies.},
language = {eng},
number = {2},
journal = {Clinical and Experimental Immunology},
author = {Van Helden, P. D. and Van Lill, L. and Bester, A. J. and De Waal, L. and Hoal-van Helden, E. G.},
month = aug,
year = {1987},
pmid = {3498594},
pmcid = {PMC1542401},
keywords = {Antibodies, Antinuclear, Arthritis, Rheumatoid, Cobalt, DNA, DNA, Single-Stranded, Humans, Lupus Erythematosus, Systemic, Polydeoxyribonucleotides, Radioimmunoassay},
pages = {394--402},
}
Downloads: 0
{"_id":"xAKhseb6bo8fLfhHY","bibbaseid":"vanhelden-vanlill-bester-dewaal-hoalvanhelden-anewassayforantidnaantibodiesinserumwhichincludesthemeasurementofantizdna-1987","downloads":0,"creationDate":"2015-12-10T11:48:57.873Z","title":"A new assay for anti-DNA antibodies in serum which includes the measurement of anti-Z-DNA","author_short":["Van Helden, P. D.","Van Lill, L.","Bester, A. J.","De Waal, L.","Hoal-van Helden, E. G."],"year":1987,"bibtype":"article","biburl":"http://bibbase.org/zotero/TBHostGen","bibdata":{"bibtype":"article","type":"article","title":"A new assay for anti-DNA antibodies in serum which includes the measurement of anti-Z-DNA","volume":"69","issn":"0009-9104","abstract":"A simple, rapid assay for measuring anti-DNA titre of serum which includes anti-Z-DNA is described. The assay involves solution binding of antibody to labelled DNA under conditions such that the DNA is altered to form a left-handed or Z-DNA structure in the presence of cobalt ions. The absence or presence of cobalt determines a B or Z form structure in DNA and antibodies to these forms are detectable. The majority of SLE and RA patients (88%) have a higher anti-DNA titre in the presence of cobalt ions. An additional 25% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. These results suggest that monitoring anti-DNA levels in SLE and RA to detect anti-Z DNA antibodies, provides significant advantages over methods currently in use to measure anti-DNA antibodies.","language":"eng","number":"2","journal":"Clinical and Experimental Immunology","author":[{"propositions":[],"lastnames":["Van","Helden"],"firstnames":["P.","D."],"suffixes":[]},{"propositions":[],"lastnames":["Van","Lill"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Bester"],"firstnames":["A.","J."],"suffixes":[]},{"propositions":[],"lastnames":["De","Waal"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Hoal-van","Helden"],"firstnames":["E.","G."],"suffixes":[]}],"month":"August","year":"1987","pmid":"3498594","pmcid":"PMC1542401","keywords":"Antibodies, Antinuclear, Arthritis, Rheumatoid, Cobalt, DNA, DNA, Single-Stranded, Humans, Lupus Erythematosus, Systemic, Polydeoxyribonucleotides, Radioimmunoassay","pages":"394–402","bibtex":"@article{van_helden_new_1987,\n\ttitle = {A new assay for anti-{DNA} antibodies in serum which includes the measurement of anti-{Z}-{DNA}},\n\tvolume = {69},\n\tissn = {0009-9104},\n\tabstract = {A simple, rapid assay for measuring anti-DNA titre of serum which includes anti-Z-DNA is described. The assay involves solution binding of antibody to labelled DNA under conditions such that the DNA is altered to form a left-handed or Z-DNA structure in the presence of cobalt ions. The absence or presence of cobalt determines a B or Z form structure in DNA and antibodies to these forms are detectable. The majority of SLE and RA patients (88\\%) have a higher anti-DNA titre in the presence of cobalt ions. An additional 25\\% of SLE patients and 22/23 RA patients who had normal anti-DNA levels according to the Crithidea assay, reacted with abnormal titres in our assay. Patients experiencing a relapse in SLE also showed a large increase in anti-DNA in the presence of antigenic Z-DNA. These results suggest that monitoring anti-DNA levels in SLE and RA to detect anti-Z DNA antibodies, provides significant advantages over methods currently in use to measure anti-DNA antibodies.},\n\tlanguage = {eng},\n\tnumber = {2},\n\tjournal = {Clinical and Experimental Immunology},\n\tauthor = {Van Helden, P. D. and Van Lill, L. and Bester, A. J. and De Waal, L. and Hoal-van Helden, E. G.},\n\tmonth = aug,\n\tyear = {1987},\n\tpmid = {3498594},\n\tpmcid = {PMC1542401},\n\tkeywords = {Antibodies, Antinuclear, Arthritis, Rheumatoid, Cobalt, DNA, DNA, Single-Stranded, Humans, Lupus Erythematosus, Systemic, Polydeoxyribonucleotides, Radioimmunoassay},\n\tpages = {394--402},\n}\n\n\n\n","author_short":["Van Helden, P. D.","Van Lill, L.","Bester, A. J.","De Waal, L.","Hoal-van Helden, E. G."],"key":"van_helden_new_1987","id":"van_helden_new_1987","bibbaseid":"vanhelden-vanlill-bester-dewaal-hoalvanhelden-anewassayforantidnaantibodiesinserumwhichincludesthemeasurementofantizdna-1987","role":"author","urls":{},"keyword":["Antibodies","Antinuclear","Arthritis","Rheumatoid","Cobalt","DNA","DNA","Single-Stranded","Humans","Lupus Erythematosus","Systemic","Polydeoxyribonucleotides","Radioimmunoassay"],"metadata":{"authorlinks":{}},"downloads":0},"search_terms":["new","assay","anti","dna","antibodies","serum","includes","measurement","anti","dna","van helden","van lill","bester","de waal","hoal-van helden"],"keywords":["antibodies","antinuclear","arthritis","rheumatoid","cobalt","dna","dna","single-stranded","humans","lupus erythematosus","systemic","polydeoxyribonucleotides","radioimmunoassay"],"authorIDs":[],"dataSources":["csPxbBZ9RG8CqYnrf"]}