A MYC2/MYC3/MYC4-dependent transcription factor network regulates water spray-responsive gene expression and jasmonate levels. Van Moerkercke, A., Duncan, O., Zander, M., Šimura, J., Broda, M., Vanden Bossche, R., Lewsey, M. G., Lama, S., Singh, K. B., Ljung, K., Ecker, J. R., Goossens, A., Millar, A. H., & Van Aken, O. Proceedings of the National Academy of Sciences, 116(46):23345–23356, November, 2019. ![A MYC2/MYC3/MYC4-dependent transcription factor network regulates water spray-responsive gene expression and jasmonate levels [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex 1 download Mechanical stimuli, such as wind, rain, and touch affect plant development, growth, pest resistance, and ultimately reproductive success. Using water spray to simulate rain, we demonstrate that jasmonic acid (JA) signaling plays a key role in early gene-expression changes, well before it leads to developmental changes in flowering and plant architecture. The JA-activated transcription factors MYC2/MYC3/MYC4 modulate transiently induced expression of 266 genes, most of which peak within 30 min, and control 52% of genes induced \textgreater100-fold. Chromatin immunoprecipitation-sequencing analysis indicates that MYC2 dynamically binds \textgreater1,300 promoters and trans -activation assays show that MYC2 activates these promoters. By mining our multiomic datasets, we identified a core MYC2/MYC3/MYC4-dependent “regulon” of 82 genes containing many previously unknown MYC2 targets, including transcription factors bHLH19 and ERF109 . bHLH19 can in turn directly activate the ORA47 promoter, indicating that MYC2/MYC3/MYC4 initiate a hierarchical network of downstream transcription factors. Finally, we also reveal that rapid water spray-induced accumulation of JA and JA-isoleucine is directly controlled by MYC2/MYC3/MYC4 through a positive amplification loop that regulates JA-biosynthesis genes.
@article{van_moerkercke_myc2myc3myc4-dependent_2019,
title = {A {MYC2}/{MYC3}/{MYC4}-dependent transcription factor network regulates water spray-responsive gene expression and jasmonate levels},
volume = {116},
issn = {0027-8424, 1091-6490},
url = {http://www.pnas.org/lookup/doi/10.1073/pnas.1911758116},
doi = {10.1073/pnas.1911758116},
abstract = {Mechanical stimuli, such as wind, rain, and touch affect plant development, growth, pest resistance, and ultimately reproductive success. Using water spray to simulate rain, we demonstrate that jasmonic acid (JA) signaling plays a key role in early gene-expression changes, well before it leads to developmental changes in flowering and plant architecture. The JA-activated transcription factors MYC2/MYC3/MYC4 modulate transiently induced expression of 266 genes, most of which peak within 30 min, and control 52\% of genes induced {\textgreater}100-fold. Chromatin immunoprecipitation-sequencing analysis indicates that MYC2 dynamically binds {\textgreater}1,300 promoters and
trans
-activation assays show that MYC2 activates these promoters. By mining our multiomic datasets, we identified a core MYC2/MYC3/MYC4-dependent “regulon” of 82 genes containing many previously unknown MYC2 targets, including transcription factors
bHLH19
and
ERF109
. bHLH19 can in turn directly activate the
ORA47
promoter, indicating that MYC2/MYC3/MYC4 initiate a hierarchical network of downstream transcription factors. Finally, we also reveal that rapid water spray-induced accumulation of JA and JA-isoleucine is directly controlled by MYC2/MYC3/MYC4 through a positive amplification loop that regulates JA-biosynthesis genes.},
language = {en},
number = {46},
urldate = {2021-06-07},
journal = {Proceedings of the National Academy of Sciences},
author = {Van Moerkercke, Alex and Duncan, Owen and Zander, Mark and Šimura, Jan and Broda, Martyna and Vanden Bossche, Robin and Lewsey, Mathew G. and Lama, Sbatie and Singh, Karam B. and Ljung, Karin and Ecker, Joseph R. and Goossens, Alain and Millar, A. Harvey and Van Aken, Olivier},
month = nov,
year = {2019},
pages = {23345--23356},
}
Downloads: 1
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Using water spray to simulate rain, we demonstrate that jasmonic acid (JA) signaling plays a key role in early gene-expression changes, well before it leads to developmental changes in flowering and plant architecture. The JA-activated transcription factors MYC2/MYC3/MYC4 modulate transiently induced expression of 266 genes, most of which peak within 30 min, and control 52% of genes induced \\textgreater100-fold. Chromatin immunoprecipitation-sequencing analysis indicates that MYC2 dynamically binds \\textgreater1,300 promoters and trans -activation assays show that MYC2 activates these promoters. By mining our multiomic datasets, we identified a core MYC2/MYC3/MYC4-dependent “regulon” of 82 genes containing many previously unknown MYC2 targets, including transcription factors bHLH19 and ERF109 . bHLH19 can in turn directly activate the ORA47 promoter, indicating that MYC2/MYC3/MYC4 initiate a hierarchical network of downstream transcription factors. 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Using water spray to simulate rain, we demonstrate that jasmonic acid (JA) signaling plays a key role in early gene-expression changes, well before it leads to developmental changes in flowering and plant architecture. The JA-activated transcription factors MYC2/MYC3/MYC4 modulate transiently induced expression of 266 genes, most of which peak within 30 min, and control 52\\% of genes induced {\\textgreater}100-fold. Chromatin immunoprecipitation-sequencing analysis indicates that MYC2 dynamically binds {\\textgreater}1,300 promoters and\n trans\n -activation assays show that MYC2 activates these promoters. By mining our multiomic datasets, we identified a core MYC2/MYC3/MYC4-dependent “regulon” of 82 genes containing many previously unknown MYC2 targets, including transcription factors\n bHLH19\n and\n ERF109\n . bHLH19 can in turn directly activate the\n ORA47\n promoter, indicating that MYC2/MYC3/MYC4 initiate a hierarchical network of downstream transcription factors. 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