Mechanical conflict caused by a cell-wall-loosening enzyme activates <i>de novo</i> shoot regeneration. Varapparambath, V., Mathew, M. M., Shanmukhan, A. P., Radhakrishnan, D., Kareem, A., Verma, S., Ramalho, J. J., Manoj, B., Vellandath, A. R., Aiyaz, M., Radha, R. K., Landge, A. N., Mähönen, A. P., Heisler, M. G., Weijers, D., & Prasad, K. Developmental Cell, 57(17):2063–2080.e10, September, 2022. ![Mechanical conflict caused by a cell-wall-loosening enzyme activates <i>de novo</i> shoot regeneration [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Cellular heterogeneity is a hallmark of multicellular organisms. During shoot regeneration from undifferentiated callus, only a select few cells, called progenitors, develop into shoot. How these cells are selected and what governs their subsequent progression to a patterned organ system is unknown. Using Arabidopsis thaliana, we show that it is not just the abundance of stem cell regulators but rather the localization pattern of polarity proteins that predicts the progenitor’s fate. A shoot-promoting factor, CUC2, activated the expression of the cell-wall-loosening enzyme, XTH9, solely in a shell of cells surrounding the progenitor, causing different mechanical stresses in these cells. This mechanical conflict then activates cell polarity in progenitors to promote meristem formation. Interestingly, genetic or physical perturbations to cells surrounding the progenitor impaired the progenitor and vice versa. These suggest a feedback loop between progenitors and their neighbors for shoot regeneration in the absence of tissue-patterning cues.
@article{varapparambath_mechanical_2022,
title = {Mechanical conflict caused by a cell-wall-loosening enzyme activates \textit{de novo} shoot regeneration},
volume = {57},
issn = {1534-5807},
url = {https://www.sciencedirect.com/science/article/pii/S1534580722005482},
doi = {10.1016/j.devcel.2022.07.017},
abstract = {Cellular heterogeneity is a hallmark of multicellular organisms. During shoot regeneration from undifferentiated callus, only a select few cells, called progenitors, develop into shoot. How these cells are selected and what governs their subsequent progression to a patterned organ system is unknown. Using Arabidopsis thaliana, we show that it is not just the abundance of stem cell regulators but rather the localization pattern of polarity proteins that predicts the progenitor’s fate. A shoot-promoting factor, CUC2, activated the expression of the cell-wall-loosening enzyme, XTH9, solely in a shell of cells surrounding the progenitor, causing different mechanical stresses in these cells. This mechanical conflict then activates cell polarity in progenitors to promote meristem formation. Interestingly, genetic or physical perturbations to cells surrounding the progenitor impaired the progenitor and vice versa. These suggest a feedback loop between progenitors and their neighbors for shoot regeneration in the absence of tissue-patterning cues.},
number = {17},
urldate = {2026-09-07},
journal = {Developmental Cell},
author = {Varapparambath, Vijina and Mathew, Mabel Maria and Shanmukhan, Anju Pallipurath and Radhakrishnan, Dhanya and Kareem, Abdul and Verma, Shubham and Ramalho, João Jacob and Manoj, Bejoy and Vellandath, Archana Rajan and Aiyaz, Mohammed and Radha, Raji Krishna and Landge, Amit N. and Mähönen, Ari Pekka and Heisler, Marcus G. and Weijers, Dolf and Prasad, Kalika},
month = sep,
year = {2022},
keywords = {CUC2, auxin, cell polarity, cell-wall-loosening enzyme, mechanical conflict, shoot regeneration},
pages = {2063--2080.e10},
}
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During shoot regeneration from undifferentiated callus, only a select few cells, called progenitors, develop into shoot. How these cells are selected and what governs their subsequent progression to a patterned organ system is unknown. Using Arabidopsis thaliana, we show that it is not just the abundance of stem cell regulators but rather the localization pattern of polarity proteins that predicts the progenitor’s fate. A shoot-promoting factor, CUC2, activated the expression of the cell-wall-loosening enzyme, XTH9, solely in a shell of cells surrounding the progenitor, causing different mechanical stresses in these cells. This mechanical conflict then activates cell polarity in progenitors to promote meristem formation. Interestingly, genetic or physical perturbations to cells surrounding the progenitor impaired the progenitor and vice versa. These suggest a feedback loop between progenitors and their neighbors for shoot regeneration in the absence of tissue-patterning cues.},\n\tnumber = {17},\n\turldate = {2026-09-07},\n\tjournal = {Developmental Cell},\n\tauthor = {Varapparambath, Vijina and Mathew, Mabel Maria and Shanmukhan, Anju Pallipurath and Radhakrishnan, Dhanya and Kareem, Abdul and Verma, Shubham and Ramalho, João Jacob and Manoj, Bejoy and Vellandath, Archana Rajan and Aiyaz, Mohammed and Radha, Raji Krishna and Landge, Amit N. and Mähönen, Ari Pekka and Heisler, Marcus G. and Weijers, Dolf and Prasad, Kalika},\n\tmonth = sep,\n\tyear = {2022},\n\tkeywords = {CUC2, auxin, cell polarity, cell-wall-loosening enzyme, mechanical conflict, shoot regeneration},\n\tpages = {2063--2080.e10},\n}\n\n\n\n","author_short":["Varapparambath, V.","Mathew, M. M.","Shanmukhan, A. P.","Radhakrishnan, D.","Kareem, A.","Verma, S.","Ramalho, J. J.","Manoj, B.","Vellandath, A. R.","Aiyaz, M.","Radha, R. K.","Landge, A. 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