@article{vaughn_nondestructive_1974,
	title = {Nondestructive acetylene reduction assays of nitrogen-fixing systems in soil ({Abstract})},
	shorttitle = {Nondestructive acetylene reduction assays of nitrogen-fixing systems in soil ({Abstract})},
	abstract = {Abstract:  A method for conducting short-duration acetylene reduction assays of intact nitrogenase systems in soil was developed.  Undisturbed cultures were maintained in 1.6 kg of soil in specially designed polyethylene pots.  The pots were sealed and the cultures exposed to an air-acetylene atmosphere which was drawn through the soil during the incubation.  No lag period was observed in the ethylene production rates of Trifolium subterraneum cultures assayed in this manner.  Successively biweekly assays of 30 or 60 minutes duration were conducted on 7 annual Trifolium species during complete growth and maturation cycles.  There was a significant linear relationship between seasonal ethylene production and both plant top N uptake and dry matter production.  An average of 4.5 moles of ethylene was produced per mole of N taken up.  Ethylene production during a 4-month period by free-living soil organisms in the rhizosphere of an annual grass, Bromus mollis, was significantly and inversely related to the amount of fertilizer N applied.  Ethylene production was significantly higher in the grass rhizosphere than in comparable fallow soils, except where 100 ppm N was applied to the grass cultures.},
	journal = {Agron. Abstr., Am. Soc. Agron., Madison, WI},
	author = {Vaughn, Charles E. and Jones, Milton B.},
	year = {1974},
	keywords = {1974, Bromus mollis, Jones, M. B., Trifolium subterraneum, Vaughn, C. E., clover, subterranean, clovers, annual, grass, annual, nitrogen fixation, estimation, soft chess, subclover},
	pages = {133}
}

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The pots were sealed and the cultures exposed to an air-acetylene atmosphere which was drawn through the soil during the incubation. No lag period was observed in the ethylene production rates of Trifolium subterraneum cultures assayed in this manner. Successively biweekly assays of 30 or 60 minutes duration were conducted on 7 annual Trifolium species during complete growth and maturation cycles. There was a significant linear relationship between seasonal ethylene production and both plant top N uptake and dry matter production. An average of 4.5 moles of ethylene was produced per mole of N taken up. Ethylene production during a 4-month period by free-living soil organisms in the rhizosphere of an annual grass, Bromus mollis, was significantly and inversely related to the amount of fertilizer N applied. Ethylene production was significantly higher in the grass rhizosphere than in comparable fallow soils, except where 100 ppm N was applied to the grass cultures.","journal":"Agron. Abstr., Am. Soc. Agron., Madison, WI","author":[{"propositions":[],"lastnames":["Vaughn"],"firstnames":["Charles","E."],"suffixes":[]},{"propositions":[],"lastnames":["Jones"],"firstnames":["Milton","B."],"suffixes":[]}],"year":"1974","keywords":"1974, Bromus mollis, Jones, M. B., Trifolium subterraneum, Vaughn, C. E., clover, subterranean, clovers, annual, grass, annual, nitrogen fixation, estimation, soft chess, subclover","pages":"133","bibtex":"@article{vaughn_nondestructive_1974,\n\ttitle = {Nondestructive acetylene reduction assays of nitrogen-fixing systems in soil ({Abstract})},\n\tshorttitle = {Nondestructive acetylene reduction assays of nitrogen-fixing systems in soil ({Abstract})},\n\tabstract = {Abstract: A method for conducting short-duration acetylene reduction assays of intact nitrogenase systems in soil was developed. Undisturbed cultures were maintained in 1.6 kg of soil in specially designed polyethylene pots. The pots were sealed and the cultures exposed to an air-acetylene atmosphere which was drawn through the soil during the incubation. No lag period was observed in the ethylene production rates of Trifolium subterraneum cultures assayed in this manner. Successively biweekly assays of 30 or 60 minutes duration were conducted on 7 annual Trifolium species during complete growth and maturation cycles. There was a significant linear relationship between seasonal ethylene production and both plant top N uptake and dry matter production. An average of 4.5 moles of ethylene was produced per mole of N taken up. Ethylene production during a 4-month period by free-living soil organisms in the rhizosphere of an annual grass, Bromus mollis, was significantly and inversely related to the amount of fertilizer N applied. 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