{"_id":"fMwMFqEkDMHaoTpfR","bibbaseid":"vestergren-vincent-jansson-persson-istedt-groebner-giesler-schleucher-highresolutioncharacterizationoforganicphosphorusinsoilextractsusing2dh1p31nmrcorrelationspectroscopy-2012","author_short":["Vestergren, J.","Vincent, A. G.","Jansson, M.","Persson, P.","Istedt, U.","Groebner, G.","Giesler, R.","Schleucher, J."],"bibdata":{"bibtype":"article","type":"article","title":"High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy","volume":"46","issn":"0013-936X","doi":"10.1021/es204016h","abstract":"Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.","language":"English","number":"7","journal":"Environmental Science & Technology","author":[{"propositions":[],"lastnames":["Vestergren"],"firstnames":["Johan"],"suffixes":[]},{"propositions":[],"lastnames":["Vincent"],"firstnames":["Andrea","G."],"suffixes":[]},{"propositions":[],"lastnames":["Jansson"],"firstnames":["Mats"],"suffixes":[]},{"propositions":[],"lastnames":["Persson"],"firstnames":["Per"],"suffixes":[]},{"propositions":[],"lastnames":["Istedt"],"firstnames":["Ulrik"],"suffixes":[]},{"propositions":[],"lastnames":["Groebner"],"firstnames":["Gerhard"],"suffixes":[]},{"propositions":[],"lastnames":["Giesler"],"firstnames":["Reiner"],"suffixes":[]},{"propositions":[],"lastnames":["Schleucher"],"firstnames":["Juergen"],"suffixes":[]}],"month":"April","year":"2012","note":"00000","keywords":"#nosource, edta, identification, nuclear-magnetic-resonance, phosphates, samples","pages":"3950–3956","bibtex":"@article{vestergren_high-resolution_2012,\n\ttitle = {High-{Resolution} {Characterization} of {Organic} {Phosphorus} in {Soil} {Extracts} {Using} {2D} {H}-1-{P}-31 {NMR} {Correlation} {Spectroscopy}},\n\tvolume = {46},\n\tissn = {0013-936X},\n\tdoi = {10.1021/es204016h},\n\tabstract = {Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.},\n\tlanguage = {English},\n\tnumber = {7},\n\tjournal = {Environmental Science \\& Technology},\n\tauthor = {Vestergren, Johan and Vincent, Andrea G. and Jansson, Mats and Persson, Per and Istedt, Ulrik and Groebner, Gerhard and Giesler, Reiner and Schleucher, Juergen},\n\tmonth = apr,\n\tyear = {2012},\n\tnote = {00000},\n\tkeywords = {\\#nosource, edta, identification, nuclear-magnetic-resonance, phosphates, samples},\n\tpages = {3950--3956},\n}\n\n\n\n","author_short":["Vestergren, J.","Vincent, A. G.","Jansson, M.","Persson, P.","Istedt, U.","Groebner, G.","Giesler, R.","Schleucher, J."],"key":"vestergren_high-resolution_2012","id":"vestergren_high-resolution_2012","bibbaseid":"vestergren-vincent-jansson-persson-istedt-groebner-giesler-schleucher-highresolutioncharacterizationoforganicphosphorusinsoilextractsusing2dh1p31nmrcorrelationspectroscopy-2012","role":"author","urls":{},"keyword":["#nosource","edta","identification","nuclear-magnetic-resonance","phosphates","samples"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/circ-publications","dataSources":["Mdrhvw5KhxFbTbWoS","nbqZWNnSmJwdJFEEB"],"keywords":["#nosource","edta","identification","nuclear-magnetic-resonance","phosphates","samples"],"search_terms":["high","resolution","characterization","organic","phosphorus","soil","extracts","using","nmr","correlation","spectroscopy","vestergren","vincent","jansson","persson","istedt","groebner","giesler","schleucher"],"title":"High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy","year":2012}