High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy

High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy. Vestergren, J., Vincent, A. G., Jansson, M., Persson, P., Istedt, U., Groebner, G., Giesler, R., & Schleucher, J. Environmental Science & Technology, 46(7):3950–3956, April, 2012. 00000
doi abstract bibtex

Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.

@article{vestergren_high-resolution_2012,
	title = {High-{Resolution} {Characterization} of {Organic} {Phosphorus} in {Soil} {Extracts} {Using} {2D} {H}-1-{P}-31 {NMR} {Correlation} {Spectroscopy}},
	volume = {46},
	issn = {0013-936X},
	doi = {10.1021/es204016h},
	abstract = {Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.},
	language = {English},
	number = {7},
	journal = {Environmental Science \& Technology},
	author = {Vestergren, Johan and Vincent, Andrea G. and Jansson, Mats and Persson, Per and Istedt, Ulrik and Groebner, Gerhard and Giesler, Reiner and Schleucher, Juergen},
	month = apr,
	year = {2012},
	note = {00000},
	keywords = {\#nosource, edta, identification, nuclear-magnetic-resonance, phosphates, samples},
	pages = {3950--3956},
}

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