Mechanism and stoichiometry of 2,2-diphenyl-1-picrylhydrazyl radical scavenging by glutathione and its novel alpha-glutamyl derivative. Viirlaid, S., Mahlapuu, R., Kilk, K., Kuznetsov, A., Soomets, U., & Järv, J. Bioorganic chemistry, 37(4):126-32, 2009. ![Mechanism and stoichiometry of 2,2-diphenyl-1-picrylhydrazyl radical scavenging by glutathione and its novel alpha-glutamyl derivative. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Website abstract bibtex Kinetic mechanism and stoichiometry of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical by glutathione and its novel analog, containing alpha-glutamyl residue in place of the gamma-glutamyl moiety, were studied using different ratios of reagents. At low concentrations of the peptides, the process was described as a bimolecular reaction obeying the stoichiometric ratio 1:1. However, at excess of peptides the formation of a non-covalent complex between the reagents was discovered and characterized by dissociation constants K = 0.61 mM for glutathione and K = 0.27 mM for the glutathione alpha-glutamyl analog, respectively. The complex formation was followed by a reaction step that was characterized by the similar rate constant k = 0.02 s(-1) for both peptides. Thus, the apparently different antioxidant activity of these two peptides, observed under common assay conditions, was determined by differences in the formation of this non-covalent complex.
@article{
title = {Mechanism and stoichiometry of 2,2-diphenyl-1-picrylhydrazyl radical scavenging by glutathione and its novel alpha-glutamyl derivative.},
type = {article},
year = {2009},
identifiers = {[object Object]},
keywords = {Biphenyl Compounds,Biphenyl Compounds: chemistry,Free Radical Scavengers,Free Radical Scavengers: chemistry,Free Radical Scavengers: metabolism,Glutathione,Glutathione: analogs & derivatives,Glutathione: chemistry,Glutathione: metabolism,Indicators and Reagents,Indicators and Reagents: chemistry,Kinetics,Peptides,Peptides: chemical synthesis,Peptides: chemistry,Picrates,Picrates: chemistry},
pages = {126-32},
volume = {37},
websites = {http://www.sciencedirect.com/science/article/pii/S0045206809000224},
id = {52044eba-001c-3c43-896d-5845216d0fa8},
created = {2016-01-28T12:02:02.000Z},
file_attached = {false},
profile_id = {dd94ede8-8057-3021-b774-e8e1019e0a90},
last_modified = {2016-01-28T12:02:03.000Z},
read = {false},
starred = {false},
authored = {true},
confirmed = {true},
hidden = {false},
abstract = {Kinetic mechanism and stoichiometry of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical by glutathione and its novel analog, containing alpha-glutamyl residue in place of the gamma-glutamyl moiety, were studied using different ratios of reagents. At low concentrations of the peptides, the process was described as a bimolecular reaction obeying the stoichiometric ratio 1:1. However, at excess of peptides the formation of a non-covalent complex between the reagents was discovered and characterized by dissociation constants K = 0.61 mM for glutathione and K = 0.27 mM for the glutathione alpha-glutamyl analog, respectively. The complex formation was followed by a reaction step that was characterized by the similar rate constant k = 0.02 s(-1) for both peptides. Thus, the apparently different antioxidant activity of these two peptides, observed under common assay conditions, was determined by differences in the formation of this non-covalent complex.},
bibtype = {article},
author = {Viirlaid, Säde and Mahlapuu, Riina and Kilk, Kalle and Kuznetsov, Aleksei and Soomets, Ursel and Järv, Jaak},
journal = {Bioorganic chemistry},
number = {4}
}
Downloads: 0
{"_id":"XzY6CqE68xAtMYvDz","bibbaseid":"viirlaid-mahlapuu-kilk-kuznetsov-soomets-jrv-mechanismandstoichiometryof22diphenyl1picrylhydrazylradicalscavengingbyglutathioneanditsnovelalphaglutamylderivative-2009","downloads":0,"creationDate":"2016-01-28T13:24:55.344Z","title":"Mechanism and stoichiometry of 2,2-diphenyl-1-picrylhydrazyl radical scavenging by glutathione and its novel alpha-glutamyl derivative.","author_short":["Viirlaid, S.","Mahlapuu, R.","Kilk, K.","Kuznetsov, A.","Soomets, U.","Järv, J."],"year":2009,"bibtype":"article","biburl":null,"bibdata":{"title":"Mechanism and stoichiometry of 2,2-diphenyl-1-picrylhydrazyl radical scavenging by glutathione and its novel alpha-glutamyl derivative.","type":"article","year":"2009","identifiers":"[object Object]","keywords":"Biphenyl Compounds,Biphenyl Compounds: chemistry,Free Radical Scavengers,Free Radical Scavengers: chemistry,Free Radical Scavengers: metabolism,Glutathione,Glutathione: analogs & derivatives,Glutathione: chemistry,Glutathione: metabolism,Indicators and Reagents,Indicators and Reagents: chemistry,Kinetics,Peptides,Peptides: chemical synthesis,Peptides: chemistry,Picrates,Picrates: chemistry","pages":"126-32","volume":"37","websites":"http://www.sciencedirect.com/science/article/pii/S0045206809000224","id":"52044eba-001c-3c43-896d-5845216d0fa8","created":"2016-01-28T12:02:02.000Z","file_attached":false,"profile_id":"dd94ede8-8057-3021-b774-e8e1019e0a90","last_modified":"2016-01-28T12:02:03.000Z","read":false,"starred":false,"authored":"true","confirmed":"true","hidden":false,"abstract":"Kinetic mechanism and stoichiometry of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical by glutathione and its novel analog, containing alpha-glutamyl residue in place of the gamma-glutamyl moiety, were studied using different ratios of reagents. At low concentrations of the peptides, the process was described as a bimolecular reaction obeying the stoichiometric ratio 1:1. However, at excess of peptides the formation of a non-covalent complex between the reagents was discovered and characterized by dissociation constants K = 0.61 mM for glutathione and K = 0.27 mM for the glutathione alpha-glutamyl analog, respectively. The complex formation was followed by a reaction step that was characterized by the similar rate constant k = 0.02 s(-1) for both peptides. Thus, the apparently different antioxidant activity of these two peptides, observed under common assay conditions, was determined by differences in the formation of this non-covalent complex.","bibtype":"article","author":"Viirlaid, Säde and Mahlapuu, Riina and Kilk, Kalle and Kuznetsov, Aleksei and Soomets, Ursel and Järv, Jaak","journal":"Bioorganic chemistry","number":"4","bibtex":"@article{\n title = {Mechanism and stoichiometry of 2,2-diphenyl-1-picrylhydrazyl radical scavenging by glutathione and its novel alpha-glutamyl derivative.},\n type = {article},\n year = {2009},\n identifiers = {[object Object]},\n keywords = {Biphenyl Compounds,Biphenyl Compounds: chemistry,Free Radical Scavengers,Free Radical Scavengers: chemistry,Free Radical Scavengers: metabolism,Glutathione,Glutathione: analogs & derivatives,Glutathione: chemistry,Glutathione: metabolism,Indicators and Reagents,Indicators and Reagents: chemistry,Kinetics,Peptides,Peptides: chemical synthesis,Peptides: chemistry,Picrates,Picrates: chemistry},\n pages = {126-32},\n volume = {37},\n websites = {http://www.sciencedirect.com/science/article/pii/S0045206809000224},\n id = {52044eba-001c-3c43-896d-5845216d0fa8},\n created = {2016-01-28T12:02:02.000Z},\n file_attached = {false},\n profile_id = {dd94ede8-8057-3021-b774-e8e1019e0a90},\n last_modified = {2016-01-28T12:02:03.000Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n abstract = {Kinetic mechanism and stoichiometry of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical by glutathione and its novel analog, containing alpha-glutamyl residue in place of the gamma-glutamyl moiety, were studied using different ratios of reagents. At low concentrations of the peptides, the process was described as a bimolecular reaction obeying the stoichiometric ratio 1:1. However, at excess of peptides the formation of a non-covalent complex between the reagents was discovered and characterized by dissociation constants K = 0.61 mM for glutathione and K = 0.27 mM for the glutathione alpha-glutamyl analog, respectively. The complex formation was followed by a reaction step that was characterized by the similar rate constant k = 0.02 s(-1) for both peptides. Thus, the apparently different antioxidant activity of these two peptides, observed under common assay conditions, was determined by differences in the formation of this non-covalent complex.},\n bibtype = {article},\n author = {Viirlaid, Säde and Mahlapuu, Riina and Kilk, Kalle and Kuznetsov, Aleksei and Soomets, Ursel and Järv, Jaak},\n journal = {Bioorganic chemistry},\n number = {4}\n}","author_short":["Viirlaid, S.","Mahlapuu, R.","Kilk, K.","Kuznetsov, A.","Soomets, U.","Järv, J."],"urls":{"Website":"http://www.sciencedirect.com/science/article/pii/S0045206809000224"},"bibbaseid":"viirlaid-mahlapuu-kilk-kuznetsov-soomets-jrv-mechanismandstoichiometryof22diphenyl1picrylhydrazylradicalscavengingbyglutathioneanditsnovelalphaglutamylderivative-2009","role":"author","keyword":["Biphenyl Compounds","Biphenyl Compounds: chemistry","Free Radical Scavengers","Free Radical Scavengers: chemistry","Free Radical Scavengers: metabolism","Glutathione","Glutathione: analogs & derivatives","Glutathione: chemistry","Glutathione: metabolism","Indicators and Reagents","Indicators and Reagents: chemistry","Kinetics","Peptides","Peptides: chemical synthesis","Peptides: chemistry","Picrates","Picrates: chemistry"],"downloads":0},"search_terms":["mechanism","stoichiometry","diphenyl","picrylhydrazyl","radical","scavenging","glutathione","novel","alpha","glutamyl","derivative","viirlaid","mahlapuu","kilk","kuznetsov","soomets","järv"],"keywords":["biphenyl compounds","biphenyl compounds: chemistry","free radical scavengers","free radical scavengers: chemistry","free radical scavengers: metabolism","glutathione","glutathione: analogs & derivatives","glutathione: chemistry","glutathione: metabolism","indicators and reagents","indicators and reagents: chemistry","kinetics","peptides","peptides: chemical synthesis","peptides: chemistry","picrates","picrates: chemistry"],"authorIDs":[]}