@ARTICLE{Vinkenborg2009-tj,
  title    = "Genetically encoded {FRET} sensors to monitor intracellular Zn2+
              homeostasis",
  author   = "Vinkenborg, Jan L and Nicolson, Tamara J and Bellomo, Elisa A and
              Koay, Melissa S and Rutter, Guy A and Merkx, Maarten",
  abstract = "We developed genetically encoded fluorescence resonance energy
              transfer (FRET)-based sensors that display a large ratiometric
              change upon Zn(2+) binding, have affinities that span the pico-
              to nanomolar range and can readily be targeted to subcellular
              organelles. Using this sensor toolbox we found that cytosolic
              Zn(2+) was buffered at 0.4 nM in pancreatic beta cells, and we
              found substantially higher Zn(2+) concentrations in
              insulin-containing secretory vesicles.",
  journal  = "Nat Methods",
  volume   =  6,
  number   =  10,
  pages    = "737--740",
  month    =  aug,
  year     =  2009,
  language = "en"
}

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