Matrix degradation in Human Immunodeficiency Virus Type 1–associated tuberculosis and tuberculosis immune reconstitution inflammatory syndrome: a prospective observational study. Walker, N. F, Wilkinson, K. A, Meintjes, G. A, Tezera, L. B, Goliath, R., Peyper, J. M, Tadokera, R., Opondo, C., Coussens, A. K, Wilkinson, R. J, Friedland, J. S, & Elkington, P. T Clinical Infectious Diseases, 65(1):121–132, Oxford University Press, jul, 2017. ![Matrix degradation in Human Immunodeficiency Virus Type 1–associated tuberculosis and tuberculosis immune reconstitution inflammatory syndrome: a prospective observational study [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Background Extensive immunopathology occurs in human immunodeficiency virus (HIV)/tuberculosis (TB) coinfection, but the underlying molecular mechanisms are not well-defined. Excessive matrix metalloproteinase (MMP) activity is emerging as a key process but has not been systematically studied in HIV-associated TB. Methods We performed a cross-sectional study of matrix turnover in HIV type 1 (HIV-1)–infected and –uninfected TB patients and controls, and a prospective cohort study of HIV-1–infected TB patients at risk of TB immune reconstitution inflammatory syndrome (TB-IRIS), in Cape Town, South Africa. Sputum and plasma MMP concentrations were quantified by Luminex, plasma procollagen III N-terminal propeptide (PIIINP) by enzyme-linked immunosorbent assay, and urinary lipoarabinomannan (LAM) by Alere Determine TB LAM assay. Peripheral blood mononuclear cells from healthy donors were cultured with Mycobacterium tuberculosis and extracellular matrix in a 3D model of TB granuloma formation. Results MMP activity differed between HIV-1–infected and –uninfected TB patients and corresponded with specific TB clinical phenotypes. HIV-1–infected TB patients had reduced pulmonary MMP concentrations, associated with reduced cavitation, but increased plasma PIIINP, compared to HIV-1–uninfected TB patients. Elevated extrapulmonary extracellular matrix turnover was associated with TB-IRIS, both before and during TB-IRIS onset. The predominant collagenase was MMP-8, which was likely neutrophil derived and M. tuberculosis–antigen driven. Mycobacterium tuberculosis–induced matrix degradation was suppressed by the MMP inhibitor doxycycline in vitro. Conclusions MMP activity in TB differs by HIV-1 status and compartment, and releases matrix degradation products. Matrix turnover in HIV-1–infected patients is increased before and during TB-IRIS, informing novel diagnostic strategies. MMP inhibition is a potential host-directed therapy strategy for prevention and treatment of TB-IRIS.
@article{Walker2017,
abstract = {Background Extensive immunopathology occurs in human immunodeficiency virus (HIV)/tuberculosis (TB) coinfection, but the underlying molecular mechanisms are not well-defined. Excessive matrix metalloproteinase (MMP) activity is emerging as a key process but has not been systematically studied in HIV-associated TB. Methods We performed a cross-sectional study of matrix turnover in HIV type 1 (HIV-1)–infected and –uninfected TB patients and controls, and a prospective cohort study of HIV-1–infected TB patients at risk of TB immune reconstitution inflammatory syndrome (TB-IRIS), in Cape Town, South Africa. Sputum and plasma MMP concentrations were quantified by Luminex, plasma procollagen III N-terminal propeptide (PIIINP) by enzyme-linked immunosorbent assay, and urinary lipoarabinomannan (LAM) by Alere Determine TB LAM assay. Peripheral blood mononuclear cells from healthy donors were cultured with Mycobacterium tuberculosis and extracellular matrix in a 3D model of TB granuloma formation. Results MMP activity differed between HIV-1–infected and –uninfected TB patients and corresponded with specific TB clinical phenotypes. HIV-1–infected TB patients had reduced pulmonary MMP concentrations, associated with reduced cavitation, but increased plasma PIIINP, compared to HIV-1–uninfected TB patients. Elevated extrapulmonary extracellular matrix turnover was associated with TB-IRIS, both before and during TB-IRIS onset. The predominant collagenase was MMP-8, which was likely neutrophil derived and M. tuberculosis–antigen driven. Mycobacterium tuberculosis–induced matrix degradation was suppressed by the MMP inhibitor doxycycline in vitro. Conclusions MMP activity in TB differs by HIV-1 status and compartment, and releases matrix degradation products. Matrix turnover in HIV-1–infected patients is increased before and during TB-IRIS, informing novel diagnostic strategies. MMP inhibition is a potential host-directed therapy strategy for prevention and treatment of TB-IRIS.},
author = {Walker, Naomi F and Wilkinson, Katalin A and Meintjes, Graeme A and Tezera, Liku B and Goliath, Ren{\'{e}} and Peyper, Janique M and Tadokera, Rebecca and Opondo, Charles and Coussens, Anna K and Wilkinson, Robert J and Friedland, Jon S and Elkington, Paul T},
doi = {10.1093/cid/cix231},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Walker et al. - 2017 - Matrix degradation in Human Immunodeficiency Virus Type 1–associated tuberculosis and tuberculosis immune reconst.pdf:pdf},
journal = {Clinical Infectious Diseases},
keywords = {HIV-1,OA,fund{\_}ack,immune reconstitution inflammatory syndrome,matrix metalloproteinase,original,procollagen III N-terminal propeptide,tuberculosis},
mendeley-tags = {OA,fund{\_}ack,original},
month = {jul},
number = {1},
pages = {121--132},
pmid = {28475709},
publisher = {Oxford University Press},
title = {{Matrix degradation in Human Immunodeficiency Virus Type 1–associated tuberculosis and tuberculosis immune reconstitution inflammatory syndrome: a prospective observational study}},
url = {https://academic.oup.com/cid/article/65/1/121/3799983},
volume = {65},
year = {2017}
}
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Methods We performed a cross-sectional study of matrix turnover in HIV type 1 (HIV-1)–infected and –uninfected TB patients and controls, and a prospective cohort study of HIV-1–infected TB patients at risk of TB immune reconstitution inflammatory syndrome (TB-IRIS), in Cape Town, South Africa. Sputum and plasma MMP concentrations were quantified by Luminex, plasma procollagen III N-terminal propeptide (PIIINP) by enzyme-linked immunosorbent assay, and urinary lipoarabinomannan (LAM) by Alere Determine TB LAM assay. Peripheral blood mononuclear cells from healthy donors were cultured with Mycobacterium tuberculosis and extracellular matrix in a 3D model of TB granuloma formation. Results MMP activity differed between HIV-1–infected and –uninfected TB patients and corresponded with specific TB clinical phenotypes. HIV-1–infected TB patients had reduced pulmonary MMP concentrations, associated with reduced cavitation, but increased plasma PIIINP, compared to HIV-1–uninfected TB patients. Elevated extrapulmonary extracellular matrix turnover was associated with TB-IRIS, both before and during TB-IRIS onset. The predominant collagenase was MMP-8, which was likely neutrophil derived and M. tuberculosis–antigen driven. Mycobacterium tuberculosis–induced matrix degradation was suppressed by the MMP inhibitor doxycycline in vitro. Conclusions MMP activity in TB differs by HIV-1 status and compartment, and releases matrix degradation products. Matrix turnover in HIV-1–infected patients is increased before and during TB-IRIS, informing novel diagnostic strategies. MMP inhibition is a potential host-directed therapy strategy for prevention and treatment of TB-IRIS.","author":[{"propositions":[],"lastnames":["Walker"],"firstnames":["Naomi","F"],"suffixes":[]},{"propositions":[],"lastnames":["Wilkinson"],"firstnames":["Katalin","A"],"suffixes":[]},{"propositions":[],"lastnames":["Meintjes"],"firstnames":["Graeme","A"],"suffixes":[]},{"propositions":[],"lastnames":["Tezera"],"firstnames":["Liku","B"],"suffixes":[]},{"propositions":[],"lastnames":["Goliath"],"firstnames":["René"],"suffixes":[]},{"propositions":[],"lastnames":["Peyper"],"firstnames":["Janique","M"],"suffixes":[]},{"propositions":[],"lastnames":["Tadokera"],"firstnames":["Rebecca"],"suffixes":[]},{"propositions":[],"lastnames":["Opondo"],"firstnames":["Charles"],"suffixes":[]},{"propositions":[],"lastnames":["Coussens"],"firstnames":["Anna","K"],"suffixes":[]},{"propositions":[],"lastnames":["Wilkinson"],"firstnames":["Robert","J"],"suffixes":[]},{"propositions":[],"lastnames":["Friedland"],"firstnames":["Jon","S"],"suffixes":[]},{"propositions":[],"lastnames":["Elkington"],"firstnames":["Paul","T"],"suffixes":[]}],"doi":"10.1093/cid/cix231","file":":C$\\$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Walker et al. - 2017 - Matrix degradation in Human Immunodeficiency Virus Type 1–associated tuberculosis and tuberculosis immune reconst.pdf:pdf","journal":"Clinical Infectious Diseases","keywords":"HIV-1,OA,fund_ack,immune reconstitution inflammatory syndrome,matrix metalloproteinase,original,procollagen III N-terminal propeptide,tuberculosis","mendeley-tags":"OA,fund_ack,original","month":"jul","number":"1","pages":"121–132","pmid":"28475709","publisher":"Oxford University Press","title":"Matrix degradation in Human Immunodeficiency Virus Type 1–associated tuberculosis and tuberculosis immune reconstitution inflammatory syndrome: a prospective observational study","url":"https://academic.oup.com/cid/article/65/1/121/3799983","volume":"65","year":"2017","bibtex":"@article{Walker2017,\r\nabstract = {Background Extensive immunopathology occurs in human immunodeficiency virus (HIV)/tuberculosis (TB) coinfection, but the underlying molecular mechanisms are not well-defined. Excessive matrix metalloproteinase (MMP) activity is emerging as a key process but has not been systematically studied in HIV-associated TB. Methods We performed a cross-sectional study of matrix turnover in HIV type 1 (HIV-1)–infected and –uninfected TB patients and controls, and a prospective cohort study of HIV-1–infected TB patients at risk of TB immune reconstitution inflammatory syndrome (TB-IRIS), in Cape Town, South Africa. Sputum and plasma MMP concentrations were quantified by Luminex, plasma procollagen III N-terminal propeptide (PIIINP) by enzyme-linked immunosorbent assay, and urinary lipoarabinomannan (LAM) by Alere Determine TB LAM assay. Peripheral blood mononuclear cells from healthy donors were cultured with Mycobacterium tuberculosis and extracellular matrix in a 3D model of TB granuloma formation. Results MMP activity differed between HIV-1–infected and –uninfected TB patients and corresponded with specific TB clinical phenotypes. HIV-1–infected TB patients had reduced pulmonary MMP concentrations, associated with reduced cavitation, but increased plasma PIIINP, compared to HIV-1–uninfected TB patients. Elevated extrapulmonary extracellular matrix turnover was associated with TB-IRIS, both before and during TB-IRIS onset. The predominant collagenase was MMP-8, which was likely neutrophil derived and M. tuberculosis–antigen driven. Mycobacterium tuberculosis–induced matrix degradation was suppressed by the MMP inhibitor doxycycline in vitro. Conclusions MMP activity in TB differs by HIV-1 status and compartment, and releases matrix degradation products. Matrix turnover in HIV-1–infected patients is increased before and during TB-IRIS, informing novel diagnostic strategies. 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