@inproceedings{wallander_comparison_nodate,
	title = {Comparison of {IHC}, {FISH} and {RT}-{PCR} for the {Detection} of {EML4}-{ALK}
Translocation {Variants} in {Non}-{Small} {Cell} {Lung} {Cancer}},
	abstract = {ARUP Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT

Department of Pathology, University of Utah Health Sciences Center and ARUP Laboratories, Salt Lake City, UT

Concordance among the three methodologies (IHC, FISH and RTPCR)
differed for the two tested EML4-ALK translocation variants.
Variant 3a/b was detectable by all three methods. Conversely,
limited ALK protein expression and subjectivity in FISH scoring
resulted in no concordance between all three methods for the
detection of EML4-ALK variant 1. Agreement among FISH readers
was poor for variant 1. This was likely due to the expected small
split in probe signals. These results suggest that the detection of
other EML4-ALK variants that involve the 3’ region of EML4 (exons
15-20) may also be challenging by FISH.
RT-PCR was the most sensitive and least subjective methodology
for EML4-ALK variant 1 detection. While multiple specific primer
sets would need to be designed for the detection of all known
EML4-ALK translocation variants from FFPE tissue by RT-PCR, this
approach would likely yield the greatest assay sensitivity.
Additionally, our results confirm that the incidence of EML4-ALK
translocations in NSCLC is greater (21\%) in enriched patient
populations.},
	author = {Wallander, Michelle L. and Geiersbach, Katherine B. and Tripp, Sheryl and Layfield, Lester J.},
}

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