@article{Wang2022,
abstract = {The generation of the stable, high-level recombinant protein-producing cell lines remains a significant challenge in the biopharmaceutical industry. Expression vector optimization is an effective strategy to increase transgene expression levels and stability, and the choice of suitable poly A element is crucial for the expression of recombinant protein. In this study, we investigated the effects of different poly A elements on transgene expression in Chinese hamster ovary (CHO) cells. Five poly A elements, including bovine growth hormone (BGH), mutant BGH, herpes simplex virus type 1 thymidine kinase (HSV-TK), SV40, and a synthetic (Synt) poly A, were cloned into the expression vector and transfected into CHO cells. The results indicated the SV40 and Synt poly A sequences can significant improve eGFP transgene expression in stable transfected CHO cells and maintain long-term expression. However, qPCR results showed that the eGFP expression at protein level was not related to the gene copy number and mRNA level. Importantly, the SV40 and Synt poly A elements decreased the variation of eGFP transgene expression. Furthermore, it also showed that the SV40 and Synt poly A elements induced higher levels of adalimumab expression. In conclusion, SV40 poly A and Synt poly A are stronger elements that increase stable transgene expression and decrease the variation of expression, and the choice of suitable poly A element is helpful to improve the expression of recombinant protein.},
author = {Wang, Xiao-yin and Du, Qiu-jie and Zhang, Wei-li and Xu, Dan-hua and Zhang, Xi and Jia, Yan-long and Wang, Tian-yun},
doi = {10.3389/fbioe.2022.722722},
issn = {2296-4185},
journal = {Frontiers in Bioengineering and Biotechnology},
keywords = {Chinese hamster ovary cells,optimization,poly A elements,stability,transgene expression},
month = {jan},
title = {{Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells}},
url = {https://www.frontiersin.org/articles/10.3389/fbioe.2022.722722/full},
volume = {10},
year = {2022}
}

{"_id":"Gs86tP6e7WAw7aqeh","bibbaseid":"wang-du-zhang-xu-zhang-jia-wang-enhancedtransgeneexpressionbyoptimizationofpolyaintransfectedchocells-2022","author_short":["Wang, X.","Du, Q.","Zhang, W.","Xu, D.","Zhang, X.","Jia, Y.","Wang, T."],"bibdata":{"bibtype":"article","type":"article","abstract":"The generation of the stable, high-level recombinant protein-producing cell lines remains a significant challenge in the biopharmaceutical industry. Expression vector optimization is an effective strategy to increase transgene expression levels and stability, and the choice of suitable poly A element is crucial for the expression of recombinant protein. In this study, we investigated the effects of different poly A elements on transgene expression in Chinese hamster ovary (CHO) cells. Five poly A elements, including bovine growth hormone (BGH), mutant BGH, herpes simplex virus type 1 thymidine kinase (HSV-TK), SV40, and a synthetic (Synt) poly A, were cloned into the expression vector and transfected into CHO cells. The results indicated the SV40 and Synt poly A sequences can significant improve eGFP transgene expression in stable transfected CHO cells and maintain long-term expression. However, qPCR results showed that the eGFP expression at protein level was not related to the gene copy number and mRNA level. Importantly, the SV40 and Synt poly A elements decreased the variation of eGFP transgene expression. Furthermore, it also showed that the SV40 and Synt poly A elements induced higher levels of adalimumab expression. In conclusion, SV40 poly A and Synt poly A are stronger elements that increase stable transgene expression and decrease the variation of expression, and the choice of suitable poly A element is helpful to improve the expression of recombinant protein.","author":[{"propositions":[],"lastnames":["Wang"],"firstnames":["Xiao-yin"],"suffixes":[]},{"propositions":[],"lastnames":["Du"],"firstnames":["Qiu-jie"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Wei-li"],"suffixes":[]},{"propositions":[],"lastnames":["Xu"],"firstnames":["Dan-hua"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Xi"],"suffixes":[]},{"propositions":[],"lastnames":["Jia"],"firstnames":["Yan-long"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Tian-yun"],"suffixes":[]}],"doi":"10.3389/fbioe.2022.722722","issn":"2296-4185","journal":"Frontiers in Bioengineering and Biotechnology","keywords":"Chinese hamster ovary cells,optimization,poly A elements,stability,transgene expression","month":"jan","title":"Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells","url":"https://www.frontiersin.org/articles/10.3389/fbioe.2022.722722/full","volume":"10","year":"2022","bibtex":"@article{Wang2022,\nabstract = {The generation of the stable, high-level recombinant protein-producing cell lines remains a significant challenge in the biopharmaceutical industry. Expression vector optimization is an effective strategy to increase transgene expression levels and stability, and the choice of suitable poly A element is crucial for the expression of recombinant protein. In this study, we investigated the effects of different poly A elements on transgene expression in Chinese hamster ovary (CHO) cells. Five poly A elements, including bovine growth hormone (BGH), mutant BGH, herpes simplex virus type 1 thymidine kinase (HSV-TK), SV40, and a synthetic (Synt) poly A, were cloned into the expression vector and transfected into CHO cells. The results indicated the SV40 and Synt poly A sequences can significant improve eGFP transgene expression in stable transfected CHO cells and maintain long-term expression. However, qPCR results showed that the eGFP expression at protein level was not related to the gene copy number and mRNA level. Importantly, the SV40 and Synt poly A elements decreased the variation of eGFP transgene expression. Furthermore, it also showed that the SV40 and Synt poly A elements induced higher levels of adalimumab expression. In conclusion, SV40 poly A and Synt poly A are stronger elements that increase stable transgene expression and decrease the variation of expression, and the choice of suitable poly A element is helpful to improve the expression of recombinant protein.},\nauthor = {Wang, Xiao-yin and Du, Qiu-jie and Zhang, Wei-li and Xu, Dan-hua and Zhang, Xi and Jia, Yan-long and Wang, Tian-yun},\ndoi = {10.3389/fbioe.2022.722722},\nissn = {2296-4185},\njournal = {Frontiers in Bioengineering and Biotechnology},\nkeywords = {Chinese hamster ovary cells,optimization,poly A elements,stability,transgene expression},\nmonth = {jan},\ntitle = {{Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells}},\nurl = {https://www.frontiersin.org/articles/10.3389/fbioe.2022.722722/full},\nvolume = {10},\nyear = {2022}\n}\n","author_short":["Wang, X.","Du, Q.","Zhang, W.","Xu, D.","Zhang, X.","Jia, Y.","Wang, T."],"key":"Wang2022","id":"Wang2022","bibbaseid":"wang-du-zhang-xu-zhang-jia-wang-enhancedtransgeneexpressionbyoptimizationofpolyaintransfectedchocells-2022","role":"author","urls":{"Paper":"https://www.frontiersin.org/articles/10.3389/fbioe.2022.722722/full"},"keyword":["Chinese hamster ovary cells","optimization","poly A elements","stability","transgene expression"],"metadata":{"authorlinks":{}},"downloads":0,"html":""},"bibtype":"article","biburl":"https://bibbase.org/network/files/ChjuT9PC4NJ7R6xJc","dataSources":["gpCKn4jBfpcFxtxyi"],"keywords":["chinese hamster ovary cells","optimization","poly a elements","stability","transgene expression"],"search_terms":["enhanced","transgene","expression","optimization","poly","transfected","cho","cells","wang","du","zhang","xu","zhang","jia","wang"],"title":"Enhanced Transgene Expression by Optimization of Poly A in Transfected CHO Cells","year":2022}