@article{ wang_resveratrol_????,
  title = {Resveratrol {Rescues} {Kidney} {Mitochondrial} {Function} {Following} {Hemorrhagic} {Shock}},
  issn = {1073-2322},
  doi = {10.1097/SHK.0000000000000390},
  abstract = {Objective: Hemorrhagic shock may contribute to acute kidney injury by profoundly altering renal mitochondrial function. Resveratrol (RSV), a naturally occurring sirtuin-1 (SIRT1) activator, has been shown to promote mitochondrial function and reduce oxidative damage in a variety of aging-related disease states. We hypothesized that RSV treatment during resuscitation would ameliorate kidney mitochondrial dysfunction and decrease oxidative damage following hemorrhagic shock., Method: Using a decompensated hemorrhagic shock model, male Long-Evans rats (n=6 per group) were sacrificed prior to hemorrhage (Sham), at severe shock, and following either lactated Ringer's (LR) Resuscitation or LR+RSV Resuscitation (RSV: 30mg/kg). At each time point, blood samples were assayed for arterial blood gases, lactate, blood urea nitrogen (BUN) and serum creatinine. Mitochondria were also isolated from kidney samples in order to assess individual electron transport complexes (CI, CII, and CIV) using high-resolution respirometry. Total mitochondria reactive oxygen species (ROS) were measured using fluorometry and lipid peroxidation was assessed by measuring 4-hydroxynonenal by Western blot. qPCR was used quantify mRNA from PGC1-[alpha], SIRT1, and proteins known to mitigate oxidative damage and promote mitochondrial biogenesis., Results: RSV supplementation during resuscitation restored mitochondrial respiratory capacity, decreased mitochondrial ROS and lipid peroxidation. Compared to standard LR resuscitation, RSV treatment significantly increased SIRT1 and PGC1-[alpha] expression and significantly increased both SOD2 and catalase expression. Although RSV was associated with decreased lactate production, pH, BUN and serum creatinine values did not differ between resuscitation strategies., Conclusions: Resuscitation with RSV significantly restored renal mitochondrial function and decreased oxidative damage following hemorrhagic shock., (C) 2015 by the Shock Society},
  language = {English.},
  journal = {Shock},
  author = {Wang, Hao and Guan, Yuxia BS and Karamercan, Mehmet Akif and Ye, Lan and Bhatti, Tricia and Becker, Lance B. and Baur, Joseph A. and Sims, Carrie A. MD},
  keywords = {resveratrol}
}

{"_id":"KtfJB4u38ZLyCzX77","bibbaseid":"wang-guan-bs-karamercan-akif-ye-bhatti-becker-baur-sims-md-resveratrolrescueskidneymitochondrialfunctionfollowinghemorrhagicshock","downloads":0,"creationDate":"2015-06-15T14:51:24.675Z","title":"Resveratrol Rescues Kidney Mitochondrial Function Following Hemorrhagic Shock","author_short":["Wang, H.","Guan","BS, Y.","Karamercan","Akif, M.","Ye, L.","Bhatti, T.","Becker, L.<nbsp>B.","Baur, J.<nbsp>A.","Sims","MD, C.<nbsp>A."],"year":null,"bibtype":"article","biburl":"http://bibbase.org/zotero/yishin0819","bibdata":{"abstract":"Objective: Hemorrhagic shock may contribute to acute kidney injury by profoundly altering renal mitochondrial function. Resveratrol (RSV), a naturally occurring sirtuin-1 (SIRT1) activator, has been shown to promote mitochondrial function and reduce oxidative damage in a variety of aging-related disease states. We hypothesized that RSV treatment during resuscitation would ameliorate kidney mitochondrial dysfunction and decrease oxidative damage following hemorrhagic shock., Method: Using a decompensated hemorrhagic shock model, male Long-Evans rats (n=6 per group) were sacrificed prior to hemorrhage (Sham), at severe shock, and following either lactated Ringer's (LR) Resuscitation or LR+RSV Resuscitation (RSV: 30mg/kg). At each time point, blood samples were assayed for arterial blood gases, lactate, blood urea nitrogen (BUN) and serum creatinine. Mitochondria were also isolated from kidney samples in order to assess individual electron transport complexes (CI, CII, and CIV) using high-resolution respirometry. Total mitochondria reactive oxygen species (ROS) were measured using fluorometry and lipid peroxidation was assessed by measuring 4-hydroxynonenal by Western blot. qPCR was used quantify mRNA from PGC1-[alpha], SIRT1, and proteins known to mitigate oxidative damage and promote mitochondrial biogenesis., Results: RSV supplementation during resuscitation restored mitochondrial respiratory capacity, decreased mitochondrial ROS and lipid peroxidation. Compared to standard LR resuscitation, RSV treatment significantly increased SIRT1 and PGC1-[alpha] expression and significantly increased both SOD2 and catalase expression. Although RSV was associated with decreased lactate production, pH, BUN and serum creatinine values did not differ between resuscitation strategies., Conclusions: Resuscitation with RSV significantly restored renal mitochondrial function and decreased oxidative damage following hemorrhagic shock., (C) 2015 by the Shock Society","author":["Wang, Hao","Guan","BS, Yuxia","Karamercan","Akif, Mehmet","Ye, Lan","Bhatti, Tricia","Becker, Lance B.","Baur, Joseph A.","Sims","MD, Carrie A."],"author_short":["Wang, H.","Guan","BS, Y.","Karamercan","Akif, M.","Ye, L.","Bhatti, T.","Becker, L.<nbsp>B.","Baur, J.<nbsp>A.","Sims","MD, C.<nbsp>A."],"bibtex":"@article{ wang_resveratrol_????,\n title = {Resveratrol {Rescues} {Kidney} {Mitochondrial} {Function} {Following} {Hemorrhagic} {Shock}},\n issn = {1073-2322},\n doi = {10.1097/SHK.0000000000000390},\n abstract = {Objective: Hemorrhagic shock may contribute to acute kidney injury by profoundly altering renal mitochondrial function. Resveratrol (RSV), a naturally occurring sirtuin-1 (SIRT1) activator, has been shown to promote mitochondrial function and reduce oxidative damage in a variety of aging-related disease states. We hypothesized that RSV treatment during resuscitation would ameliorate kidney mitochondrial dysfunction and decrease oxidative damage following hemorrhagic shock., Method: Using a decompensated hemorrhagic shock model, male Long-Evans rats (n=6 per group) were sacrificed prior to hemorrhage (Sham), at severe shock, and following either lactated Ringer's (LR) Resuscitation or LR+RSV Resuscitation (RSV: 30mg/kg). At each time point, blood samples were assayed for arterial blood gases, lactate, blood urea nitrogen (BUN) and serum creatinine. Mitochondria were also isolated from kidney samples in order to assess individual electron transport complexes (CI, CII, and CIV) using high-resolution respirometry. Total mitochondria reactive oxygen species (ROS) were measured using fluorometry and lipid peroxidation was assessed by measuring 4-hydroxynonenal by Western blot. qPCR was used quantify mRNA from PGC1-[alpha], SIRT1, and proteins known to mitigate oxidative damage and promote mitochondrial biogenesis., Results: RSV supplementation during resuscitation restored mitochondrial respiratory capacity, decreased mitochondrial ROS and lipid peroxidation. Compared to standard LR resuscitation, RSV treatment significantly increased SIRT1 and PGC1-[alpha] expression and significantly increased both SOD2 and catalase expression. Although RSV was associated with decreased lactate production, pH, BUN and serum creatinine values did not differ between resuscitation strategies., Conclusions: Resuscitation with RSV significantly restored renal mitochondrial function and decreased oxidative damage following hemorrhagic shock., (C) 2015 by the Shock Society},\n language = {English.},\n journal = {Shock},\n author = {Wang, Hao and Guan, Yuxia BS and Karamercan, Mehmet Akif and Ye, Lan and Bhatti, Tricia and Becker, Lance B. and Baur, Joseph A. and Sims, Carrie A. MD},\n keywords = {resveratrol}\n}","bibtype":"article","doi":"10.1097/SHK.0000000000000390","id":"wang_resveratrol_????","issn":"1073-2322","journal":"Shock","key":"wang_resveratrol_????","keywords":"resveratrol","language":"English.","title":"Resveratrol Rescues Kidney Mitochondrial Function Following Hemorrhagic Shock","type":"article","bibbaseid":"wang-guan-bs-karamercan-akif-ye-bhatti-becker-baur-sims-md-resveratrolrescueskidneymitochondrialfunctionfollowinghemorrhagicshock","role":"author","urls":{},"keyword":["resveratrol"],"downloads":0},"search_terms":["resveratrol","rescues","kidney","mitochondrial","function","following","hemorrhagic","shock","wang","guan","bs","karamercan","akif","ye","bhatti","becker","baur","sims","md"],"keywords":["resveratrol"],"authorIDs":[],"dataSources":["5a9x8gDFLeNBehhXX"]}